Flow Cytometric DNA Index and Karyotype in Childhood Lymphoblastic Leukemia
Flow cytometric DNA-index (DIFCM) and karyotype were analysed in 82 consecutive children with acute lymphoblastic leukemia (ALL) during a 10 year period. A statistically significant correlation existed between modal chromosome number and DIFCM (p = 0.009). DIFCM could reliably identify leukemias wit...
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| Format: | Article |
| Language: | English |
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Wiley
1998-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/1998/712042 |
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| author | Erik Forestier Gösta Holmgren Göran Roos |
| author_facet | Erik Forestier Gösta Holmgren Göran Roos |
| author_sort | Erik Forestier |
| collection | DOAJ |
| description | Flow cytometric DNA-index (DIFCM) and karyotype were analysed in 82 consecutive children with acute lymphoblastic leukemia (ALL) during a 10 year period. A statistically significant correlation existed between modal chromosome number and DIFCM (p = 0.009). DIFCM could reliably identify leukemias with >51 chromosomes, whereas only three out of 12 cases with modal chromosome numbers between 47–51 were classified as aneuploid by DIFCM. In the pseudodiploid group only one out of 20 leukemias had a DIFCM>1.0. Five leukemias with a diploid karyotype showed an aneuploid DIFCM and in three patients the flow cytometric measurement revealed biclonality undetected by karyotyping. During treatment aneuploid clones could be detected by DIFCM in a substantial number of cases where the cytogenetic analysis was normal, and the opposite was also demonstrated in one case. DIFCM gave prognostic information, showing that cases with a DI >1.12 (corresponding to 51 chromosomes) had a superior outcome with treatment protocols today in use. |
| format | Article |
| id | doaj-art-6af1a191e8ab4f7ab66176cfde980ca9 |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 1998-01-01 |
| publisher | Wiley |
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| series | Analytical Cellular Pathology |
| spelling | doaj-art-6af1a191e8ab4f7ab66176cfde980ca92025-02-03T05:58:52ZengWileyAnalytical Cellular Pathology0921-89121878-36511998-01-0117314515610.1155/1998/712042Flow Cytometric DNA Index and Karyotype in Childhood Lymphoblastic LeukemiaErik Forestier0Gösta Holmgren1Göran Roos2Department of Pediatrics, Umeå University, SwedenDepartment of Clinical Genetics, Umeå University, SwedenDepartment of Pathology, Umeå University, SwedenFlow cytometric DNA-index (DIFCM) and karyotype were analysed in 82 consecutive children with acute lymphoblastic leukemia (ALL) during a 10 year period. A statistically significant correlation existed between modal chromosome number and DIFCM (p = 0.009). DIFCM could reliably identify leukemias with >51 chromosomes, whereas only three out of 12 cases with modal chromosome numbers between 47–51 were classified as aneuploid by DIFCM. In the pseudodiploid group only one out of 20 leukemias had a DIFCM>1.0. Five leukemias with a diploid karyotype showed an aneuploid DIFCM and in three patients the flow cytometric measurement revealed biclonality undetected by karyotyping. During treatment aneuploid clones could be detected by DIFCM in a substantial number of cases where the cytogenetic analysis was normal, and the opposite was also demonstrated in one case. DIFCM gave prognostic information, showing that cases with a DI >1.12 (corresponding to 51 chromosomes) had a superior outcome with treatment protocols today in use.http://dx.doi.org/10.1155/1998/712042 |
| spellingShingle | Erik Forestier Gösta Holmgren Göran Roos Flow Cytometric DNA Index and Karyotype in Childhood Lymphoblastic Leukemia Analytical Cellular Pathology |
| title | Flow Cytometric DNA Index and Karyotype in Childhood Lymphoblastic Leukemia |
| title_full | Flow Cytometric DNA Index and Karyotype in Childhood Lymphoblastic Leukemia |
| title_fullStr | Flow Cytometric DNA Index and Karyotype in Childhood Lymphoblastic Leukemia |
| title_full_unstemmed | Flow Cytometric DNA Index and Karyotype in Childhood Lymphoblastic Leukemia |
| title_short | Flow Cytometric DNA Index and Karyotype in Childhood Lymphoblastic Leukemia |
| title_sort | flow cytometric dna index and karyotype in childhood lymphoblastic leukemia |
| url | http://dx.doi.org/10.1155/1998/712042 |
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