Decreased Frequency of Circulating Myelin Oligodendrocyte Glycoprotein B Lymphocytes in Patients with Relapsing-Remitting Multiple Sclerosis
Although there is no evidence for a role of anti-MOG antibodies in adult MS, no information on B lymphocytes with MOG-committed BCR is available. We report here on the frequency of anti-MOG B cells forming rosettes with polystyrene beads (BBR) covalently bound to the extracellular domain of rhMOG in...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/673503 |
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| author | Annie Elong Ngono Maud Lepetit Markus Reindl Alexandra Garcia Flora Guillot Athénaïs Genty Mélanie Chesneau Marion Salou Laure Michel Fabienne Lefrere Kathrin Schanda Berthe-Marie Imbert-Marcille Nicolas Degauque Arnaud Nicot Sophie Brouard David-Axel Laplaud Jean-Paul Soulillou |
| author_facet | Annie Elong Ngono Maud Lepetit Markus Reindl Alexandra Garcia Flora Guillot Athénaïs Genty Mélanie Chesneau Marion Salou Laure Michel Fabienne Lefrere Kathrin Schanda Berthe-Marie Imbert-Marcille Nicolas Degauque Arnaud Nicot Sophie Brouard David-Axel Laplaud Jean-Paul Soulillou |
| author_sort | Annie Elong Ngono |
| collection | DOAJ |
| description | Although there is no evidence for a role of anti-MOG antibodies in adult MS, no information on B lymphocytes with MOG-committed BCR is available. We report here on the frequency of anti-MOG B cells forming rosettes with polystyrene beads (BBR) covalently bound to the extracellular domain of rhMOG in 38 relapsing-remitting patients (RRMS) and 50 healthy individuals (HI). We show a substantial proportion of circulating anti-MOG-BBR in both RRMS and HI. Strikingly, MOG-specific B cells frequencies were lower in MS than in HI. Anti-MOG antibodies measured by a cell-based assay were not different between MS patients and controls, suggesting a specific alteration of anti-MOG B cells in MS. Although anti-MOG-BBR were higher in CNS fluid than in blood, no difference was observed between MS and controls. Lower frequency of MOG-BBR in MS was not explained by an increased apoptosis, but a trend for lower proliferative capacity was noted. Despite an efficient B cell transmigration across brain derived endothelial cells, total and anti-MOG B cells transmigration was similar between MS and HI. The striking alteration in MOG-specific B cells, independent of anti-MOG antibody titers, challenges our view on the role of MOG-specific B cells in MS. |
| format | Article |
| id | doaj-art-6ae697d266744128b8d799aebc99bf4b |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-6ae697d266744128b8d799aebc99bf4b2025-02-03T07:24:16ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/673503673503Decreased Frequency of Circulating Myelin Oligodendrocyte Glycoprotein B Lymphocytes in Patients with Relapsing-Remitting Multiple SclerosisAnnie Elong Ngono0Maud Lepetit1Markus Reindl2Alexandra Garcia3Flora Guillot4Athénaïs Genty5Mélanie Chesneau6Marion Salou7Laure Michel8Fabienne Lefrere9Kathrin Schanda10Berthe-Marie Imbert-Marcille11Nicolas Degauque12Arnaud Nicot13Sophie Brouard14David-Axel Laplaud15Jean-Paul Soulillou16INSERM, UMR1064, 44093 Nantes, FranceINSERM, UMR1064, 44093 Nantes, FranceClinical Department of Neurology, Innsbruck Medical University, 6020 Innsbruck, AustriaINSERM, UMR1064, 44093 Nantes, FranceINSERM, UMR1064, 44093 Nantes, FranceINSERM, UMR1064, 44093 Nantes, FranceINSERM, UMR1064, 44093 Nantes, FranceINSERM, UMR1064, 44093 Nantes, FranceINSERM, UMR1064, 44093 Nantes, FranceINSERM, CIC 004, 44093 Nantes, FranceClinical Department of Neurology, Innsbruck Medical University, 6020 Innsbruck, AustriaFaculté de Médecine, Université de Nantes, 44035 Nantes, FranceINSERM, UMR1064, 44093 Nantes, FranceINSERM, UMR1064, 44093 Nantes, FranceINSERM, UMR1064, 44093 Nantes, FranceINSERM, UMR1064, 44093 Nantes, FranceINSERM, UMR1064, 44093 Nantes, FranceAlthough there is no evidence for a role of anti-MOG antibodies in adult MS, no information on B lymphocytes with MOG-committed BCR is available. We report here on the frequency of anti-MOG B cells forming rosettes with polystyrene beads (BBR) covalently bound to the extracellular domain of rhMOG in 38 relapsing-remitting patients (RRMS) and 50 healthy individuals (HI). We show a substantial proportion of circulating anti-MOG-BBR in both RRMS and HI. Strikingly, MOG-specific B cells frequencies were lower in MS than in HI. Anti-MOG antibodies measured by a cell-based assay were not different between MS patients and controls, suggesting a specific alteration of anti-MOG B cells in MS. Although anti-MOG-BBR were higher in CNS fluid than in blood, no difference was observed between MS and controls. Lower frequency of MOG-BBR in MS was not explained by an increased apoptosis, but a trend for lower proliferative capacity was noted. Despite an efficient B cell transmigration across brain derived endothelial cells, total and anti-MOG B cells transmigration was similar between MS and HI. The striking alteration in MOG-specific B cells, independent of anti-MOG antibody titers, challenges our view on the role of MOG-specific B cells in MS.http://dx.doi.org/10.1155/2015/673503 |
| spellingShingle | Annie Elong Ngono Maud Lepetit Markus Reindl Alexandra Garcia Flora Guillot Athénaïs Genty Mélanie Chesneau Marion Salou Laure Michel Fabienne Lefrere Kathrin Schanda Berthe-Marie Imbert-Marcille Nicolas Degauque Arnaud Nicot Sophie Brouard David-Axel Laplaud Jean-Paul Soulillou Decreased Frequency of Circulating Myelin Oligodendrocyte Glycoprotein B Lymphocytes in Patients with Relapsing-Remitting Multiple Sclerosis Journal of Immunology Research |
| title | Decreased Frequency of Circulating Myelin Oligodendrocyte Glycoprotein B Lymphocytes in Patients with Relapsing-Remitting Multiple Sclerosis |
| title_full | Decreased Frequency of Circulating Myelin Oligodendrocyte Glycoprotein B Lymphocytes in Patients with Relapsing-Remitting Multiple Sclerosis |
| title_fullStr | Decreased Frequency of Circulating Myelin Oligodendrocyte Glycoprotein B Lymphocytes in Patients with Relapsing-Remitting Multiple Sclerosis |
| title_full_unstemmed | Decreased Frequency of Circulating Myelin Oligodendrocyte Glycoprotein B Lymphocytes in Patients with Relapsing-Remitting Multiple Sclerosis |
| title_short | Decreased Frequency of Circulating Myelin Oligodendrocyte Glycoprotein B Lymphocytes in Patients with Relapsing-Remitting Multiple Sclerosis |
| title_sort | decreased frequency of circulating myelin oligodendrocyte glycoprotein b lymphocytes in patients with relapsing remitting multiple sclerosis |
| url | http://dx.doi.org/10.1155/2015/673503 |
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