The Chemical Composition of Endotoxin Isolated from Intestinal Strain of Desulfovibrio desulfuricans
Desulfovibrio desulfuricans anaerobes are constituents of human alimentary tract microflora. There are suggestions that they take part in the pathogenesis of periodontitis and some gastrointestinal inflammatory disorders, such as ulcerative colitis or Crohn’s disease. Endotoxin is one of Gram-negati...
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2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/647352 |
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| author | Jolanta Lodowska Daniel Wolny Marzena Jaworska-Kik Sławomir Kurkiewicz Zofia Dzierżewicz Ludmiła Węglarz |
| author_facet | Jolanta Lodowska Daniel Wolny Marzena Jaworska-Kik Sławomir Kurkiewicz Zofia Dzierżewicz Ludmiła Węglarz |
| author_sort | Jolanta Lodowska |
| collection | DOAJ |
| description | Desulfovibrio desulfuricans anaerobes are constituents of human alimentary tract microflora. There are suggestions that they take part in the pathogenesis of periodontitis and some gastrointestinal inflammatory disorders, such as ulcerative colitis or Crohn’s disease. Endotoxin is one of Gram-negative bacteria cellular components that influence these microorganisms pathogenicity. Endotoxin is a lipid-polisaccharide heteropolymer consisting of three elements: lipid A, core oligosaccharide, and O-specific polysaccharide, also called antigen-O. The biological activity of lipopolysaccharide (LPS) is determined by its structure. In this study, we show that rhamnose, fucose, mannose, glucose, galactose, heptose, and 2-keto-3-deoxyoctulosonic acid (Kdo) are constituents of D. desulfuricans endotoxin oligosaccharide core and O-antigen. Lipid A of these bacteria LPS is composed of glucosamine disaccharide substituted by 3-acyloxyacyl residues: ester-bound 3-(dodecanoyloxy)tetradecanoic, 3-(hexadecanoyloxy)tetradecanoic acid, and amide-bound 3-(tetradecanoyloxy)tetradecanoic acid. |
| format | Article |
| id | doaj-art-6aaf89fe94c247b389b8e6f1180d7e6c |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-6aaf89fe94c247b389b8e6f1180d7e6c2025-02-03T01:24:32ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/647352647352The Chemical Composition of Endotoxin Isolated from Intestinal Strain of Desulfovibrio desulfuricansJolanta Lodowska0Daniel Wolny1Marzena Jaworska-Kik2Sławomir Kurkiewicz3Zofia Dzierżewicz4Ludmiła Węglarz5Department of Biochemistry, Faculty of Pharmacy, Medical University of Silesia, Narcyzow 1 street, 41-200 Sosnowiec, PolandDepartment of Biopharmacy, Faculty of Pharmacy, Medical University of Silesia, Narcyzow 1 street, 41-200 Sosnowiec, PolandDepartment of Biopharmacy, Faculty of Pharmacy, Medical University of Silesia, Narcyzow 1 street, 41-200 Sosnowiec, PolandDepartment of Instrumental Analysis, Faculty of Pharmacy, Medical University of Silesia, Narcyzow 1 street, 41-200 Sosnowiec, PolandDepartment of Biopharmacy, Faculty of Pharmacy, Medical University of Silesia, Narcyzow 1 street, 41-200 Sosnowiec, PolandDepartment of Biochemistry, Faculty of Pharmacy, Medical University of Silesia, Narcyzow 1 street, 41-200 Sosnowiec, PolandDesulfovibrio desulfuricans anaerobes are constituents of human alimentary tract microflora. There are suggestions that they take part in the pathogenesis of periodontitis and some gastrointestinal inflammatory disorders, such as ulcerative colitis or Crohn’s disease. Endotoxin is one of Gram-negative bacteria cellular components that influence these microorganisms pathogenicity. Endotoxin is a lipid-polisaccharide heteropolymer consisting of three elements: lipid A, core oligosaccharide, and O-specific polysaccharide, also called antigen-O. The biological activity of lipopolysaccharide (LPS) is determined by its structure. In this study, we show that rhamnose, fucose, mannose, glucose, galactose, heptose, and 2-keto-3-deoxyoctulosonic acid (Kdo) are constituents of D. desulfuricans endotoxin oligosaccharide core and O-antigen. Lipid A of these bacteria LPS is composed of glucosamine disaccharide substituted by 3-acyloxyacyl residues: ester-bound 3-(dodecanoyloxy)tetradecanoic, 3-(hexadecanoyloxy)tetradecanoic acid, and amide-bound 3-(tetradecanoyloxy)tetradecanoic acid.http://dx.doi.org/10.1100/2012/647352 |
| spellingShingle | Jolanta Lodowska Daniel Wolny Marzena Jaworska-Kik Sławomir Kurkiewicz Zofia Dzierżewicz Ludmiła Węglarz The Chemical Composition of Endotoxin Isolated from Intestinal Strain of Desulfovibrio desulfuricans The Scientific World Journal |
| title | The Chemical Composition of Endotoxin Isolated from Intestinal Strain of Desulfovibrio desulfuricans |
| title_full | The Chemical Composition of Endotoxin Isolated from Intestinal Strain of Desulfovibrio desulfuricans |
| title_fullStr | The Chemical Composition of Endotoxin Isolated from Intestinal Strain of Desulfovibrio desulfuricans |
| title_full_unstemmed | The Chemical Composition of Endotoxin Isolated from Intestinal Strain of Desulfovibrio desulfuricans |
| title_short | The Chemical Composition of Endotoxin Isolated from Intestinal Strain of Desulfovibrio desulfuricans |
| title_sort | chemical composition of endotoxin isolated from intestinal strain of desulfovibrio desulfuricans |
| url | http://dx.doi.org/10.1100/2012/647352 |
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