Evaluation of post-thaw quality of brown-swiss and holstein bull semen diluted with different extenders
<b>Aim:</b> To evaluate post-thaw guality of Brown-Swiss and Holstein bull semen diluted with different extenders.<p> <b>Materials and Methods:</b> Ejaculates obtained from Holstein (n=36) and Brown-Swiss (n=36) bulls were divided into three aliquots and diluted with Tr...
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Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=559 |
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| author | Serpil Sariozkan Purhan Barbaros Tuncer Mustafa Numan Bucak Serhat Buyukleblebici Huseyin Kinet Ali Bilgen |
| author_facet | Serpil Sariozkan Purhan Barbaros Tuncer Mustafa Numan Bucak Serhat Buyukleblebici Huseyin Kinet Ali Bilgen |
| author_sort | Serpil Sariozkan |
| collection | DOAJ |
| description | <b>Aim:</b> To evaluate post-thaw guality of Brown-Swiss and
Holstein bull semen diluted with different extenders.<p>
<b>Materials and Methods:</b> Ejaculates obtained from Holstein
(n=36) and Brown-Swiss (n=36) bulls were divided into
three aliquots and diluted with Tris-based, Optidyl<sup>®</sup> and Bioxcell<sup>®</sup> extenders, respectively. Thereafter they were frozen
and thawed following a standard protocol. The effectiveness
of freezing extenders was assessed according to postthaw
sperm motility, acrosomal and total abnormalities and
plasma membrane integrity.<p>
<b>Results:</b> With respect to Holstein bull semen, the highest
percentages of sperm subjective (p<0.01), CASA progressive
(p<0.001), and CASA motility (p<0.001) were found in
semen diluted with Optidyl<sup>®</sup>. Optidyl<sup>®</sup> extender also provided
best protection in terms of acrosome and plasma membrane
integrity compared to other extenders (p<0.001). For
motility motion including VAP, VSL and LIN values, the highest
values were obtained from Optidyl and Tris (p<0.05).
With respect to, the lowest percentages of post-thaw subjective
(p<0.01), CASA motility (p<0.001) and membrane
integrity (p<0.001) were obtained in the semen samples diluted
with Bioxcell<sup>®</sup>. The percentage of progressive motility
was found to be higher in Optidyl<sup>®</sup> than Bioxcell<sup>®</sup> (p<0.01).
The highest percentages of acrosomal and total abnormalities
were found in semen diluted with Bioxcell and Tris extenders.
The highest ALH value was obtained from Optidyl<sup>®</sup>
extender compared to the other groups (p<0.05).<p>
<b>Conclusion:</b> Optidyl<sup>®</sup> extender may be preferred rather
than Bioxcell<sup>®</sup> or Tris + egg yolk extenders for freezing the
Holstein and Brown-Swiss bull semen. |
| format | Article |
| id | doaj-art-6a89f108cee84836b5b94faf4121d482 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-6a89f108cee84836b5b94faf4121d4822025-02-03T11:25:42ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532615155559Evaluation of post-thaw quality of brown-swiss and holstein bull semen diluted with different extendersSerpil Sariozkan0Purhan Barbaros Tuncer1Mustafa Numan Bucak2Serhat Buyukleblebici3Huseyin Kinet4Ali Bilgen5Erciyes University, Safiye Cikrikcioglu Vocational College, Kayseri, TurkeyMinistry of Agriculture and Rural Affairs, Lalahan Livestock Central Research Institute, Ankara, TurkeyMinistry of Agriculture and Rural Affairs, Lalahan Livestock Central Research Institute, Ankara, TurkeyMinistry of Agriculture and Rural Affairs, Lalahan Livestock Central Research Institute, Ankara, TurkeyMinistry of Agriculture and Rural Affairs, Lalahan Livestock Central Research Institute, Ankara, TurkeyMinistry of Agriculture and Rural Affairs, Lalahan Livestock Central Research Institute, Ankara, Turkey<b>Aim:</b> To evaluate post-thaw guality of Brown-Swiss and Holstein bull semen diluted with different extenders.<p> <b>Materials and Methods:</b> Ejaculates obtained from Holstein (n=36) and Brown-Swiss (n=36) bulls were divided into three aliquots and diluted with Tris-based, Optidyl<sup>®</sup> and Bioxcell<sup>®</sup> extenders, respectively. Thereafter they were frozen and thawed following a standard protocol. The effectiveness of freezing extenders was assessed according to postthaw sperm motility, acrosomal and total abnormalities and plasma membrane integrity.<p> <b>Results:</b> With respect to Holstein bull semen, the highest percentages of sperm subjective (p<0.01), CASA progressive (p<0.001), and CASA motility (p<0.001) were found in semen diluted with Optidyl<sup>®</sup>. Optidyl<sup>®</sup> extender also provided best protection in terms of acrosome and plasma membrane integrity compared to other extenders (p<0.001). For motility motion including VAP, VSL and LIN values, the highest values were obtained from Optidyl and Tris (p<0.05). With respect to, the lowest percentages of post-thaw subjective (p<0.01), CASA motility (p<0.001) and membrane integrity (p<0.001) were obtained in the semen samples diluted with Bioxcell<sup>®</sup>. The percentage of progressive motility was found to be higher in Optidyl<sup>®</sup> than Bioxcell<sup>®</sup> (p<0.01). The highest percentages of acrosomal and total abnormalities were found in semen diluted with Bioxcell and Tris extenders. The highest ALH value was obtained from Optidyl<sup>®</sup> extender compared to the other groups (p<0.05).<p> <b>Conclusion:</b> Optidyl<sup>®</sup> extender may be preferred rather than Bioxcell<sup>®</sup> or Tris + egg yolk extenders for freezing the Holstein and Brown-Swiss bull semen.http://eurasianjvetsci.org/pdf.php3?id=559bull semencryopreservationextendermembrane integrityseminal parameters |
| spellingShingle | Serpil Sariozkan Purhan Barbaros Tuncer Mustafa Numan Bucak Serhat Buyukleblebici Huseyin Kinet Ali Bilgen Evaluation of post-thaw quality of brown-swiss and holstein bull semen diluted with different extenders Eurasian Journal of Veterinary Sciences bull semen cryopreservation extender membrane integrity seminal parameters |
| title | Evaluation of post-thaw quality of brown-swiss and holstein bull semen diluted with different extenders |
| title_full | Evaluation of post-thaw quality of brown-swiss and holstein bull semen diluted with different extenders |
| title_fullStr | Evaluation of post-thaw quality of brown-swiss and holstein bull semen diluted with different extenders |
| title_full_unstemmed | Evaluation of post-thaw quality of brown-swiss and holstein bull semen diluted with different extenders |
| title_short | Evaluation of post-thaw quality of brown-swiss and holstein bull semen diluted with different extenders |
| title_sort | evaluation of post thaw quality of brown swiss and holstein bull semen diluted with different extenders |
| topic | bull semen cryopreservation extender membrane integrity seminal parameters |
| url | http://eurasianjvetsci.org/pdf.php3?id=559 |
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