Evaluation of post-thaw quality of brown-swiss and holstein bull semen diluted with different extenders
<b>Aim:</b> To evaluate post-thaw guality of Brown-Swiss and Holstein bull semen diluted with different extenders.<p> <b>Materials and Methods:</b> Ejaculates obtained from Holstein (n=36) and Brown-Swiss (n=36) bulls were divided into three aliquots and diluted with Tr...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
|
| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=559 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | <b>Aim:</b> To evaluate post-thaw guality of Brown-Swiss and
Holstein bull semen diluted with different extenders.<p>
<b>Materials and Methods:</b> Ejaculates obtained from Holstein
(n=36) and Brown-Swiss (n=36) bulls were divided into
three aliquots and diluted with Tris-based, Optidyl<sup>®</sup> and Bioxcell<sup>®</sup> extenders, respectively. Thereafter they were frozen
and thawed following a standard protocol. The effectiveness
of freezing extenders was assessed according to postthaw
sperm motility, acrosomal and total abnormalities and
plasma membrane integrity.<p>
<b>Results:</b> With respect to Holstein bull semen, the highest
percentages of sperm subjective (p<0.01), CASA progressive
(p<0.001), and CASA motility (p<0.001) were found in
semen diluted with Optidyl<sup>®</sup>. Optidyl<sup>®</sup> extender also provided
best protection in terms of acrosome and plasma membrane
integrity compared to other extenders (p<0.001). For
motility motion including VAP, VSL and LIN values, the highest
values were obtained from Optidyl and Tris (p<0.05).
With respect to, the lowest percentages of post-thaw subjective
(p<0.01), CASA motility (p<0.001) and membrane
integrity (p<0.001) were obtained in the semen samples diluted
with Bioxcell<sup>®</sup>. The percentage of progressive motility
was found to be higher in Optidyl<sup>®</sup> than Bioxcell<sup>®</sup> (p<0.01).
The highest percentages of acrosomal and total abnormalities
were found in semen diluted with Bioxcell and Tris extenders.
The highest ALH value was obtained from Optidyl<sup>®</sup>
extender compared to the other groups (p<0.05).<p>
<b>Conclusion:</b> Optidyl<sup>®</sup> extender may be preferred rather
than Bioxcell<sup>®</sup> or Tris + egg yolk extenders for freezing the
Holstein and Brown-Swiss bull semen. |
|---|---|
| ISSN: | 1309-6958 2146-1953 |