Optimization of nutrient medium composition for BHK-21/2-17 suspension culture
The paper demonstrates results of the research aimed at optimization of amino acid composition of the nutrient media used for BHK-21-2/17 suspension cultivation by change of amino acid amount in such media. Growth level in the control and tested media amounted to 5.6; cell growth dynamics was also s...
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| Format: | Article |
| Language: | English |
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Da Vinci Media
2018-04-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/270 |
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| author | M. N. Guseva D. V. Mikhalishin A. A. Shishkova D. S. Bolshakov B. L. Manin M. A. Shevchenko |
| author_facet | M. N. Guseva D. V. Mikhalishin A. A. Shishkova D. S. Bolshakov B. L. Manin M. A. Shevchenko |
| author_sort | M. N. Guseva |
| collection | DOAJ |
| description | The paper demonstrates results of the research aimed at optimization of amino acid composition of the nutrient media used for BHK-21-2/17 suspension cultivation by change of amino acid amount in such media. Growth level in the control and tested media amounted to 5.6; cell growth dynamics was also similar. In-media utilization of different amino acids was different. The cells mostly consumed glutamin, serine, tyrosine, methionin (up to 90%, 55%, 40% and 60%, respectively). Growth of alanine level was reported (from 10% in the control sample and up to 60% in the test sample). Amount of tryptophan and glutamic acid remained stable. Glycine, threonine, isoleucine were actively utilized within the first 24 hours (7-13%, 10-18%, 34-41%, respectively in the control experiment). Hereafter, the concentration slightly increased (by 7-45%, by 16-21%, by 20-50%, respectively in the control experiment). During the FMDV reproduction in the cells grown in the test and control media no significant differences in the yield of immunogenic virus components were reported. |
| format | Article |
| id | doaj-art-6a795c371509474083905224541bba2f |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2018-04-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-6a795c371509474083905224541bba2f2025-02-06T09:52:06ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592018-04-01043539270Optimization of nutrient medium composition for BHK-21/2-17 suspension cultureM. N. Guseva0D. V. Mikhalishin1A. A. Shishkova2D. S. Bolshakov3B. L. Manin4M. A. Shevchenko5FGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirThe paper demonstrates results of the research aimed at optimization of amino acid composition of the nutrient media used for BHK-21-2/17 suspension cultivation by change of amino acid amount in such media. Growth level in the control and tested media amounted to 5.6; cell growth dynamics was also similar. In-media utilization of different amino acids was different. The cells mostly consumed glutamin, serine, tyrosine, methionin (up to 90%, 55%, 40% and 60%, respectively). Growth of alanine level was reported (from 10% in the control sample and up to 60% in the test sample). Amount of tryptophan and glutamic acid remained stable. Glycine, threonine, isoleucine were actively utilized within the first 24 hours (7-13%, 10-18%, 34-41%, respectively in the control experiment). Hereafter, the concentration slightly increased (by 7-45%, by 16-21%, by 20-50%, respectively in the control experiment). During the FMDV reproduction in the cells grown in the test and control media no significant differences in the yield of immunogenic virus components were reported.https://veterinary.arriah.ru/jour/article/view/270клетки внк-21/2-17аминокислотыаминокислотный составгидролизат белков кровипитательная средаbhk-21/2-17 cellsamino acidsamino acid compositionblood-derived protein hydrolysatenutrient medium |
| spellingShingle | M. N. Guseva D. V. Mikhalishin A. A. Shishkova D. S. Bolshakov B. L. Manin M. A. Shevchenko Optimization of nutrient medium composition for BHK-21/2-17 suspension culture Ветеринария сегодня клетки внк-21/2-17 аминокислоты аминокислотный состав гидролизат белков крови питательная среда bhk-21/2-17 cells amino acids amino acid composition blood-derived protein hydrolysate nutrient medium |
| title | Optimization of nutrient medium composition for BHK-21/2-17 suspension culture |
| title_full | Optimization of nutrient medium composition for BHK-21/2-17 suspension culture |
| title_fullStr | Optimization of nutrient medium composition for BHK-21/2-17 suspension culture |
| title_full_unstemmed | Optimization of nutrient medium composition for BHK-21/2-17 suspension culture |
| title_short | Optimization of nutrient medium composition for BHK-21/2-17 suspension culture |
| title_sort | optimization of nutrient medium composition for bhk 21 2 17 suspension culture |
| topic | клетки внк-21/2-17 аминокислоты аминокислотный состав гидролизат белков крови питательная среда bhk-21/2-17 cells amino acids amino acid composition blood-derived protein hydrolysate nutrient medium |
| url | https://veterinary.arriah.ru/jour/article/view/270 |
| work_keys_str_mv | AT mnguseva optimizationofnutrientmediumcompositionforbhk21217suspensionculture AT dvmikhalishin optimizationofnutrientmediumcompositionforbhk21217suspensionculture AT aashishkova optimizationofnutrientmediumcompositionforbhk21217suspensionculture AT dsbolshakov optimizationofnutrientmediumcompositionforbhk21217suspensionculture AT blmanin optimizationofnutrientmediumcompositionforbhk21217suspensionculture AT mashevchenko optimizationofnutrientmediumcompositionforbhk21217suspensionculture |