High-Resolution Northern Blot for a Reliable Analysis of MicroRNAs and Their Precursors
This protocol describes how to perform northern blot analyses to detect microRNAs and their precursors with single-nucleotide resolution, which is crucial for analyzing individual length variants and for evaluating relative quantities of unique microRNAs in cells. Northern blot analysis consists of...
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Main Authors: | Edyta Koscianska, Julia Starega-Roslan, Katarzyna Czubala, Wlodzimierz J. Krzyzosiak |
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Format: | Article |
Language: | English |
Published: |
Wiley
2011-01-01
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Series: | The Scientific World Journal |
Online Access: | http://dx.doi.org/10.1100/tsw.2011.11 |
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