Natural variation of CTB5 confers cold adaptation in plateau japonica rice
Abstract During cold acclimation in high-latitude and high-altitude regions, japonica rice develops enhanced cold tolerance, but the underlying genetic basis remains unclear. Here, we identify CTB5, a homeodomain-leucine zipper (HD-Zip) transcription factor that confers cold tolerance at the booting...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-56174-5 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832585571060416512 |
|---|---|
| author | Haifeng Guo Shilei Gao Huahui Li Jiazhen Yang Jin Li Yunsong Gu Qijin Lou Runbin Su Wei Ye Andong Zou Yulong Wang Xingming Sun Zhanying Zhang Hongliang Zhang Yawen Zeng Pingrong Yuan Youliang Peng Zichao Li Jinjie Li |
| author_facet | Haifeng Guo Shilei Gao Huahui Li Jiazhen Yang Jin Li Yunsong Gu Qijin Lou Runbin Su Wei Ye Andong Zou Yulong Wang Xingming Sun Zhanying Zhang Hongliang Zhang Yawen Zeng Pingrong Yuan Youliang Peng Zichao Li Jinjie Li |
| author_sort | Haifeng Guo |
| collection | DOAJ |
| description | Abstract During cold acclimation in high-latitude and high-altitude regions, japonica rice develops enhanced cold tolerance, but the underlying genetic basis remains unclear. Here, we identify CTB5, a homeodomain-leucine zipper (HD-Zip) transcription factor that confers cold tolerance at the booting stage in japonica rice. Four natural variations in the promoter and coding regions enhance cold response and transcriptional regulatory activity, enabling the favorable CTB5 KM allele to improve cold tolerance. CTB5 interacts with OsHox12 and targets gibberellin (GA) metabolism genes to promote GAs accumulation in anthers and facilitate tapetum development under cold stress. Moreover, CTB5 directly regulates PYL9 and improves cold tolerance at the seedling stage by reducing reactive oxygen species (ROS) accumulation. The CTB5 KM allele is selected during the cold acclimation of japonica rice to plateau habitats in Yunnan Province. Our findings provide insights into the mechanisms underlying cold adaptation in plateau japonica rice and offer potential targets for breeding cold-tolerant rice varieties. |
| format | Article |
| id | doaj-art-6a675e6d78924203aaa3ba0c2a4be7b6 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-6a675e6d78924203aaa3ba0c2a4be7b62025-01-26T12:41:41ZengNature PortfolioNature Communications2041-17232025-01-0116111810.1038/s41467-025-56174-5Natural variation of CTB5 confers cold adaptation in plateau japonica riceHaifeng Guo0Shilei Gao1Huahui Li2Jiazhen Yang3Jin Li4Yunsong Gu5Qijin Lou6Runbin Su7Wei Ye8Andong Zou9Yulong Wang10Xingming Sun11Zhanying Zhang12Hongliang Zhang13Yawen Zeng14Pingrong Yuan15Youliang Peng16Zichao Li17Jinjie Li18Frontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural UniversityInstitute of Food Crop Research, Yunnan Academy of Agricultural SciencesBiotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural SciencesFrontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural UniversityBiotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural SciencesInstitute of Food Crop Research, Yunnan Academy of Agricultural SciencesMinistry of Agriculture Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural UniversityAbstract During cold acclimation in high-latitude and high-altitude regions, japonica rice develops enhanced cold tolerance, but the underlying genetic basis remains unclear. Here, we identify CTB5, a homeodomain-leucine zipper (HD-Zip) transcription factor that confers cold tolerance at the booting stage in japonica rice. Four natural variations in the promoter and coding regions enhance cold response and transcriptional regulatory activity, enabling the favorable CTB5 KM allele to improve cold tolerance. CTB5 interacts with OsHox12 and targets gibberellin (GA) metabolism genes to promote GAs accumulation in anthers and facilitate tapetum development under cold stress. Moreover, CTB5 directly regulates PYL9 and improves cold tolerance at the seedling stage by reducing reactive oxygen species (ROS) accumulation. The CTB5 KM allele is selected during the cold acclimation of japonica rice to plateau habitats in Yunnan Province. Our findings provide insights into the mechanisms underlying cold adaptation in plateau japonica rice and offer potential targets for breeding cold-tolerant rice varieties.https://doi.org/10.1038/s41467-025-56174-5 |
| spellingShingle | Haifeng Guo Shilei Gao Huahui Li Jiazhen Yang Jin Li Yunsong Gu Qijin Lou Runbin Su Wei Ye Andong Zou Yulong Wang Xingming Sun Zhanying Zhang Hongliang Zhang Yawen Zeng Pingrong Yuan Youliang Peng Zichao Li Jinjie Li Natural variation of CTB5 confers cold adaptation in plateau japonica rice Nature Communications |
| title | Natural variation of CTB5 confers cold adaptation in plateau japonica rice |
| title_full | Natural variation of CTB5 confers cold adaptation in plateau japonica rice |
| title_fullStr | Natural variation of CTB5 confers cold adaptation in plateau japonica rice |
| title_full_unstemmed | Natural variation of CTB5 confers cold adaptation in plateau japonica rice |
| title_short | Natural variation of CTB5 confers cold adaptation in plateau japonica rice |
| title_sort | natural variation of ctb5 confers cold adaptation in plateau japonica rice |
| url | https://doi.org/10.1038/s41467-025-56174-5 |
| work_keys_str_mv | AT haifengguo naturalvariationofctb5conferscoldadaptationinplateaujaponicarice AT shileigao naturalvariationofctb5conferscoldadaptationinplateaujaponicarice AT huahuili naturalvariationofctb5conferscoldadaptationinplateaujaponicarice AT jiazhenyang naturalvariationofctb5conferscoldadaptationinplateaujaponicarice AT jinli naturalvariationofctb5conferscoldadaptationinplateaujaponicarice AT yunsonggu naturalvariationofctb5conferscoldadaptationinplateaujaponicarice AT qijinlou naturalvariationofctb5conferscoldadaptationinplateaujaponicarice AT runbinsu naturalvariationofctb5conferscoldadaptationinplateaujaponicarice AT weiye naturalvariationofctb5conferscoldadaptationinplateaujaponicarice AT andongzou naturalvariationofctb5conferscoldadaptationinplateaujaponicarice AT yulongwang naturalvariationofctb5conferscoldadaptationinplateaujaponicarice AT xingmingsun naturalvariationofctb5conferscoldadaptationinplateaujaponicarice AT zhanyingzhang naturalvariationofctb5conferscoldadaptationinplateaujaponicarice AT hongliangzhang naturalvariationofctb5conferscoldadaptationinplateaujaponicarice AT yawenzeng naturalvariationofctb5conferscoldadaptationinplateaujaponicarice AT pingrongyuan naturalvariationofctb5conferscoldadaptationinplateaujaponicarice AT youliangpeng naturalvariationofctb5conferscoldadaptationinplateaujaponicarice AT zichaoli naturalvariationofctb5conferscoldadaptationinplateaujaponicarice AT jinjieli naturalvariationofctb5conferscoldadaptationinplateaujaponicarice |