First Trials of Genome Analyses in Some Onobrychis Species using Dot-Blot and Genomic in situ Hybridization Techniques
The origin and genome composition of tetraploid cultivated Onobrychis viciifolia (2n = 4x = 28) were analyzed using dot-blot and genomic in situ hybridization (GISH) techniques. Dot-blot hybridization was used to find a genomic affinity between O. viciifolia and 16 diploid Onobrychis species. The hy...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Çanakkale Onsekiz Mart University
2024-06-01
|
| Series: | Journal of Advanced Research in Natural and Applied Sciences |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/en/download/article-file/3696390 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832095389234233344 |
|---|---|
| author | Metin Tuna Evren Cabi Bozena Kolano Gülru Yücel |
| author_facet | Metin Tuna Evren Cabi Bozena Kolano Gülru Yücel |
| author_sort | Metin Tuna |
| collection | DOAJ |
| description | The origin and genome composition of tetraploid cultivated Onobrychis viciifolia (2n = 4x = 28) were analyzed using dot-blot and genomic in situ hybridization (GISH) techniques. Dot-blot hybridization was used to find a genomic affinity between O. viciifolia and 16 diploid Onobrychis species. The hypothesis on the origin of the O. viciifolia was tested using GISH. Dot-blot analyses suggested a genomic affinity between O. viciifolia and four diploid Onobrychis species (O. kachetica, O. supina, O. pallasii, and O. vaginalis). Hybridization signals were observed on O. viciifolia chromosomes when gDNA of O. kachetica, O. supina, O. pallasi, and O. hypargyrea were used as probes. However, the observed chromosomal distribution of hybridization signals did not resemble GISH results. The observed signals colocalized with 35S rDNA or dispearse signals on all chromosomes were observed depending on the probe. Further investigations using more comprehensive and comparative analysis with both coding and repetitive DNA regions may provide a better understanding of the genome composition and evolution of O. viciifolia. |
| format | Article |
| id | doaj-art-6a1d0e53ce9e45f5a185aa1249d92fd4 |
| institution | Kabale University |
| issn | 2757-5195 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Çanakkale Onsekiz Mart University |
| record_format | Article |
| series | Journal of Advanced Research in Natural and Applied Sciences |
| spelling | doaj-art-6a1d0e53ce9e45f5a185aa1249d92fd42025-02-05T18:13:03ZengÇanakkale Onsekiz Mart UniversityJournal of Advanced Research in Natural and Applied Sciences2757-51952024-06-0110242643410.28979/jarnas.1428996453First Trials of Genome Analyses in Some Onobrychis Species using Dot-Blot and Genomic in situ Hybridization TechniquesMetin Tuna0https://orcid.org/0000-0003-4841-8871Evren Cabi1https://orcid.org/0000-0002-7706-5801Bozena Kolano2https://orcid.org/0000-0002-4187-3807Gülru Yücel3https://orcid.org/0000-0001-9785-929XTEKIRDAG NAMIK KEMAL UNIVERSITYTEKIRDAG NAMIK KEMAL UNIVERSITYUniversity of SilesiaOndokuz Mayıs ÜniversitesiThe origin and genome composition of tetraploid cultivated Onobrychis viciifolia (2n = 4x = 28) were analyzed using dot-blot and genomic in situ hybridization (GISH) techniques. Dot-blot hybridization was used to find a genomic affinity between O. viciifolia and 16 diploid Onobrychis species. The hypothesis on the origin of the O. viciifolia was tested using GISH. Dot-blot analyses suggested a genomic affinity between O. viciifolia and four diploid Onobrychis species (O. kachetica, O. supina, O. pallasii, and O. vaginalis). Hybridization signals were observed on O. viciifolia chromosomes when gDNA of O. kachetica, O. supina, O. pallasi, and O. hypargyrea were used as probes. However, the observed chromosomal distribution of hybridization signals did not resemble GISH results. The observed signals colocalized with 35S rDNA or dispearse signals on all chromosomes were observed depending on the probe. Further investigations using more comprehensive and comparative analysis with both coding and repetitive DNA regions may provide a better understanding of the genome composition and evolution of O. viciifolia.https://dergipark.org.tr/en/download/article-file/3696390onobrychisdot-blot hybridizationgishfish |
| spellingShingle | Metin Tuna Evren Cabi Bozena Kolano Gülru Yücel First Trials of Genome Analyses in Some Onobrychis Species using Dot-Blot and Genomic in situ Hybridization Techniques Journal of Advanced Research in Natural and Applied Sciences onobrychis dot-blot hybridization gish fish |
| title | First Trials of Genome Analyses in Some Onobrychis Species using Dot-Blot and Genomic in situ Hybridization Techniques |
| title_full | First Trials of Genome Analyses in Some Onobrychis Species using Dot-Blot and Genomic in situ Hybridization Techniques |
| title_fullStr | First Trials of Genome Analyses in Some Onobrychis Species using Dot-Blot and Genomic in situ Hybridization Techniques |
| title_full_unstemmed | First Trials of Genome Analyses in Some Onobrychis Species using Dot-Blot and Genomic in situ Hybridization Techniques |
| title_short | First Trials of Genome Analyses in Some Onobrychis Species using Dot-Blot and Genomic in situ Hybridization Techniques |
| title_sort | first trials of genome analyses in some onobrychis species using dot blot and genomic in situ hybridization techniques |
| topic | onobrychis dot-blot hybridization gish fish |
| url | https://dergipark.org.tr/en/download/article-file/3696390 |
| work_keys_str_mv | AT metintuna firsttrialsofgenomeanalysesinsomeonobrychisspeciesusingdotblotandgenomicinsituhybridizationtechniques AT evrencabi firsttrialsofgenomeanalysesinsomeonobrychisspeciesusingdotblotandgenomicinsituhybridizationtechniques AT bozenakolano firsttrialsofgenomeanalysesinsomeonobrychisspeciesusingdotblotandgenomicinsituhybridizationtechniques AT gulruyucel firsttrialsofgenomeanalysesinsomeonobrychisspeciesusingdotblotandgenomicinsituhybridizationtechniques |