Cytotoxicity and Genotoxicity of Biogenic Silver Nanoparticles in A549 and BEAS-2B Cell Lines
Introduction. Biogenic silver nanoparticles (AgNPs-GA) were successfully synthesised using Garcinia atroviridis leaf extract as a reducing agent, which has ethnopharmacological claims against various diseases including cancer. Aim of the Study. Aim of the study is to discover whether AgNPs-GA has cy...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2022/8546079 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832551810882076672 |
|---|---|
| author | Musthahimah Muhamad Nurhidayah Ab.Rahim Wan Adnan Wan Omar Nik Nur Syazni Nik Mohamed Kamal |
| author_facet | Musthahimah Muhamad Nurhidayah Ab.Rahim Wan Adnan Wan Omar Nik Nur Syazni Nik Mohamed Kamal |
| author_sort | Musthahimah Muhamad |
| collection | DOAJ |
| description | Introduction. Biogenic silver nanoparticles (AgNPs-GA) were successfully synthesised using Garcinia atroviridis leaf extract as a reducing agent, which has ethnopharmacological claims against various diseases including cancer. Aim of the Study. Aim of the study is to discover whether AgNPs-GA has cytotoxic and genotoxic effects on cancerous (A549) and noncancerous (BEAS-2B) human lung cells. Materials and Methods. The cytotoxicity profiles of AgNPs-GA were characterized by MTT assay, intracellular reactive oxygen species (ROS) assay, and DAPI and AOPI double staining, whilst genotoxicity was assessed using Comet Assay analysis. The level of silver ions (Ag+) and cellular uptake of AgNPs-GA were evaluated by ICP-OES and TEM analyses, respectively. Results. A significant cytotoxic effect was observed by AgNPs-GA on both A549 and BEAS-2B cell lines, with IC50 values of 20–28 μg/ml and 12–35 μg/ml, respectively. The cytotoxicity profile of AgNPs-GA was also accompanied by a pronounced increase in ROS production, DNA damage, and apoptosis. Moreover, Ag+ was also detected in cells exposed to AgNPs-GA threefold higher compared to controls. In this study, AgNPs-GA were endocytosed within lysosomes, which may direct to secondary toxicity effects including oxidative stress, impairment of the cell membrane, DNA fragmentation, and cell death. Conclusions. Taken together, novel toxicological-related mechanisms by AgNPs-GA were proposed involving the generation of ROS that causes DNA damage which led to programmed cell death in both A549 and BEAS-2B cells. Therefore, a combination of scientific assessments is constantly needed to ensure that the quality of biosynthesized nanoparticles is controlled and their safe development is promoted. |
| format | Article |
| id | doaj-art-6a10db47995d4805944baa2c1f32987f |
| institution | Kabale University |
| issn | 1687-479X |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-6a10db47995d4805944baa2c1f32987f2025-02-03T06:00:27ZengWileyBioinorganic Chemistry and Applications1687-479X2022-01-01202210.1155/2022/8546079Cytotoxicity and Genotoxicity of Biogenic Silver Nanoparticles in A549 and BEAS-2B Cell LinesMusthahimah Muhamad0Nurhidayah Ab.Rahim1Wan Adnan Wan Omar2Nik Nur Syazni Nik Mohamed Kamal3Department of ToxicologyDepartment of ToxicologyDepartment of ToxicologyDepartment of ToxicologyIntroduction. Biogenic silver nanoparticles (AgNPs-GA) were successfully synthesised using Garcinia atroviridis leaf extract as a reducing agent, which has ethnopharmacological claims against various diseases including cancer. Aim of the Study. Aim of the study is to discover whether AgNPs-GA has cytotoxic and genotoxic effects on cancerous (A549) and noncancerous (BEAS-2B) human lung cells. Materials and Methods. The cytotoxicity profiles of AgNPs-GA were characterized by MTT assay, intracellular reactive oxygen species (ROS) assay, and DAPI and AOPI double staining, whilst genotoxicity was assessed using Comet Assay analysis. The level of silver ions (Ag+) and cellular uptake of AgNPs-GA were evaluated by ICP-OES and TEM analyses, respectively. Results. A significant cytotoxic effect was observed by AgNPs-GA on both A549 and BEAS-2B cell lines, with IC50 values of 20–28 μg/ml and 12–35 μg/ml, respectively. The cytotoxicity profile of AgNPs-GA was also accompanied by a pronounced increase in ROS production, DNA damage, and apoptosis. Moreover, Ag+ was also detected in cells exposed to AgNPs-GA threefold higher compared to controls. In this study, AgNPs-GA were endocytosed within lysosomes, which may direct to secondary toxicity effects including oxidative stress, impairment of the cell membrane, DNA fragmentation, and cell death. Conclusions. Taken together, novel toxicological-related mechanisms by AgNPs-GA were proposed involving the generation of ROS that causes DNA damage which led to programmed cell death in both A549 and BEAS-2B cells. Therefore, a combination of scientific assessments is constantly needed to ensure that the quality of biosynthesized nanoparticles is controlled and their safe development is promoted.http://dx.doi.org/10.1155/2022/8546079 |
| spellingShingle | Musthahimah Muhamad Nurhidayah Ab.Rahim Wan Adnan Wan Omar Nik Nur Syazni Nik Mohamed Kamal Cytotoxicity and Genotoxicity of Biogenic Silver Nanoparticles in A549 and BEAS-2B Cell Lines Bioinorganic Chemistry and Applications |
| title | Cytotoxicity and Genotoxicity of Biogenic Silver Nanoparticles in A549 and BEAS-2B Cell Lines |
| title_full | Cytotoxicity and Genotoxicity of Biogenic Silver Nanoparticles in A549 and BEAS-2B Cell Lines |
| title_fullStr | Cytotoxicity and Genotoxicity of Biogenic Silver Nanoparticles in A549 and BEAS-2B Cell Lines |
| title_full_unstemmed | Cytotoxicity and Genotoxicity of Biogenic Silver Nanoparticles in A549 and BEAS-2B Cell Lines |
| title_short | Cytotoxicity and Genotoxicity of Biogenic Silver Nanoparticles in A549 and BEAS-2B Cell Lines |
| title_sort | cytotoxicity and genotoxicity of biogenic silver nanoparticles in a549 and beas 2b cell lines |
| url | http://dx.doi.org/10.1155/2022/8546079 |
| work_keys_str_mv | AT musthahimahmuhamad cytotoxicityandgenotoxicityofbiogenicsilvernanoparticlesina549andbeas2bcelllines AT nurhidayahabrahim cytotoxicityandgenotoxicityofbiogenicsilvernanoparticlesina549andbeas2bcelllines AT wanadnanwanomar cytotoxicityandgenotoxicityofbiogenicsilvernanoparticlesina549andbeas2bcelllines AT niknursyazninikmohamedkamal cytotoxicityandgenotoxicityofbiogenicsilvernanoparticlesina549andbeas2bcelllines |