Orientin and vitexin attenuate lipopolysaccharide-induced inflammatory responses in RAW264.7 cells: a molecular docking study, biochemical characterization, and mechanism analysis
ABSTRACT: Plant flavonoids have received considerable attention for their health benefits. However, structure-activity relationships between flavonoids such as orientin and vitexin with similar structures are rarely reported. In the present study, molecular docking study suggested that orientin and...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2022-09-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022000623 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832557172860387328 |
|---|---|
| author | Yue Yu Fusheng Pei Zhanming Li |
| author_facet | Yue Yu Fusheng Pei Zhanming Li |
| author_sort | Yue Yu |
| collection | DOAJ |
| description | ABSTRACT: Plant flavonoids have received considerable attention for their health benefits. However, structure-activity relationships between flavonoids such as orientin and vitexin with similar structures are rarely reported. In the present study, molecular docking study suggested that orientin and vitexin suppressed inflammatory responses through the modulation of the mitogen-activated protein kinase (MAPK) signaling pathway. Moreover, RAW264.7 cells with lipopolysaccharide-induced inflammation were used to evaluate the anti-inflammatory activities of orientin and vitexin, based on the inflammation-related cytokines production, quantitative real-time reverse transcription PCR, and western blotting analysis. As a result, orientin or vitexin attenuated inflammatory responses through modulation of the MAPK/NF-κB signaling pathway by suppressing NF-κB translocation. |
| format | Article |
| id | doaj-art-69f1431137af442897f49b3fbbb5fc4c |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-69f1431137af442897f49b3fbbb5fc4c2025-02-03T05:38:22ZengTsinghua University PressFood Science and Human Wellness2213-45302022-09-0111512731281Orientin and vitexin attenuate lipopolysaccharide-induced inflammatory responses in RAW264.7 cells: a molecular docking study, biochemical characterization, and mechanism analysisYue Yu0Fusheng Pei1Zhanming Li2School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212004, ChinaSchool of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212004, ChinaCorresponding author.; School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212004, ChinaABSTRACT: Plant flavonoids have received considerable attention for their health benefits. However, structure-activity relationships between flavonoids such as orientin and vitexin with similar structures are rarely reported. In the present study, molecular docking study suggested that orientin and vitexin suppressed inflammatory responses through the modulation of the mitogen-activated protein kinase (MAPK) signaling pathway. Moreover, RAW264.7 cells with lipopolysaccharide-induced inflammation were used to evaluate the anti-inflammatory activities of orientin and vitexin, based on the inflammation-related cytokines production, quantitative real-time reverse transcription PCR, and western blotting analysis. As a result, orientin or vitexin attenuated inflammatory responses through modulation of the MAPK/NF-κB signaling pathway by suppressing NF-κB translocation.http://www.sciencedirect.com/science/article/pii/S2213453022000623Anti-inflammatoryMitogen-activated protein kinasePlant extractNF-κBMolecular docking |
| spellingShingle | Yue Yu Fusheng Pei Zhanming Li Orientin and vitexin attenuate lipopolysaccharide-induced inflammatory responses in RAW264.7 cells: a molecular docking study, biochemical characterization, and mechanism analysis Food Science and Human Wellness Anti-inflammatory Mitogen-activated protein kinase Plant extract NF-κB Molecular docking |
| title | Orientin and vitexin attenuate lipopolysaccharide-induced inflammatory responses in RAW264.7 cells: a molecular docking study, biochemical characterization, and mechanism analysis |
| title_full | Orientin and vitexin attenuate lipopolysaccharide-induced inflammatory responses in RAW264.7 cells: a molecular docking study, biochemical characterization, and mechanism analysis |
| title_fullStr | Orientin and vitexin attenuate lipopolysaccharide-induced inflammatory responses in RAW264.7 cells: a molecular docking study, biochemical characterization, and mechanism analysis |
| title_full_unstemmed | Orientin and vitexin attenuate lipopolysaccharide-induced inflammatory responses in RAW264.7 cells: a molecular docking study, biochemical characterization, and mechanism analysis |
| title_short | Orientin and vitexin attenuate lipopolysaccharide-induced inflammatory responses in RAW264.7 cells: a molecular docking study, biochemical characterization, and mechanism analysis |
| title_sort | orientin and vitexin attenuate lipopolysaccharide induced inflammatory responses in raw264 7 cells a molecular docking study biochemical characterization and mechanism analysis |
| topic | Anti-inflammatory Mitogen-activated protein kinase Plant extract NF-κB Molecular docking |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022000623 |
| work_keys_str_mv | AT yueyu orientinandvitexinattenuatelipopolysaccharideinducedinflammatoryresponsesinraw2647cellsamoleculardockingstudybiochemicalcharacterizationandmechanismanalysis AT fushengpei orientinandvitexinattenuatelipopolysaccharideinducedinflammatoryresponsesinraw2647cellsamoleculardockingstudybiochemicalcharacterizationandmechanismanalysis AT zhanmingli orientinandvitexinattenuatelipopolysaccharideinducedinflammatoryresponsesinraw2647cellsamoleculardockingstudybiochemicalcharacterizationandmechanismanalysis |