A comparison of different cryoprotectant solutions and thawing methods for cryo­preservation of embryos of mice and rats

A comparison of different cryoprotectant solutions and thawing methods for cryo­preservation of embryos of mice and rats

The proper choice of cryoprotectant and thawing method affects cryopreservation efficiency. A freezing-thawing method for sparing embryonic cells was evaluated in experiments with ICR mice. Cleavage-stage embryos of ICR mice, GC rats, and OXYS rats were collected on Day 3 of pregnancy and frozen in...

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Bibliographic Details
Main Authors: T. N. Igonina, E. Yu. Brusentsev, I. N. Rozhkova, V. A. Naprimerov, S. Ya. Amstislavsky
Format: Article
Language:English
Published: Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders 2015-12-01
Series:Вавиловский журнал генетики и селекции
Subjects:
icr mice
gc rats
oxys rats
embryo cryopreservation
ethylene glycol
glycerol
sucrose
Online Access:https://vavilov.elpub.ru/jour/article/view/421
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