A comparison of different cryoprotectant solutions and thawing methods for cryopreservation of embryos of mice and rats
The proper choice of cryoprotectant and thawing method affects cryopreservation efficiency. A freezing-thawing method for sparing embryonic cells was evaluated in experiments with ICR mice. Cleavage-stage embryos of ICR mice, GC rats, and OXYS rats were collected on Day 3 of pregnancy and frozen in...
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| Format: | Article |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2015-12-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/421 |
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| author | T. N. Igonina E. Yu. Brusentsev I. N. Rozhkova V. A. Naprimerov S. Ya. Amstislavsky |
| author_facet | T. N. Igonina E. Yu. Brusentsev I. N. Rozhkova V. A. Naprimerov S. Ya. Amstislavsky |
| author_sort | T. N. Igonina |
| collection | DOAJ |
| description | The proper choice of cryoprotectant and thawing method affects cryopreservation efficiency. A freezing-thawing method for sparing embryonic cells was evaluated in experiments with ICR mice. Cleavage-stage embryos of ICR mice, GC rats, and OXYS rats were collected on Day 3 of pregnancy and frozen in plastic straws according to a standard protocol. Permeating (ethylene glycol and glycerol) and nonpermeating (sucrose) cryoprotectants and their combinations were compared during the freezing of ICR mouse embryos. With these mice, two thawing methods were compared: rapid (water bath, 10 s, 37 °С) and slow (40 s, room temperature; 40 s, 30 °С). Embryo viability in mice and rats was evaluated by their in vitro culturing after thawing. Our data on mice indicate that slow thawing is more suitable for sparing the integrity of embryonic cells; moreover, supplementation of the main cryoprotectant (either ethylene glycol or glycerol) with sucrose is beneficial for subsequent in vitro culture, especially in the case of glycerol. This freezing-thawing protocol (with glycerol and sucrose as cryoprotectant agents and slow thawing) was applied to rats of the GC and OXYS strains; the survival rate after cryopreservation was 68–83.3 %, and the rate of in vitro development was 64.7–66.6 %. |
| format | Article |
| id | doaj-art-69d4b6c993934cbab8800635962d4cbd |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2015-12-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-69d4b6c993934cbab8800635962d4cbd2025-02-01T09:58:02ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-12-0119437838210.18699/VJ15.047380A comparison of different cryoprotectant solutions and thawing methods for cryopreservation of embryos of mice and ratsT. N. Igonina0E. Yu. Brusentsev1I. N. Rozhkova2V. A. Naprimerov3S. Ya. Amstislavsky4Institute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaThe proper choice of cryoprotectant and thawing method affects cryopreservation efficiency. A freezing-thawing method for sparing embryonic cells was evaluated in experiments with ICR mice. Cleavage-stage embryos of ICR mice, GC rats, and OXYS rats were collected on Day 3 of pregnancy and frozen in plastic straws according to a standard protocol. Permeating (ethylene glycol and glycerol) and nonpermeating (sucrose) cryoprotectants and their combinations were compared during the freezing of ICR mouse embryos. With these mice, two thawing methods were compared: rapid (water bath, 10 s, 37 °С) and slow (40 s, room temperature; 40 s, 30 °С). Embryo viability in mice and rats was evaluated by their in vitro culturing after thawing. Our data on mice indicate that slow thawing is more suitable for sparing the integrity of embryonic cells; moreover, supplementation of the main cryoprotectant (either ethylene glycol or glycerol) with sucrose is beneficial for subsequent in vitro culture, especially in the case of glycerol. This freezing-thawing protocol (with glycerol and sucrose as cryoprotectant agents and slow thawing) was applied to rats of the GC and OXYS strains; the survival rate after cryopreservation was 68–83.3 %, and the rate of in vitro development was 64.7–66.6 %.https://vavilov.elpub.ru/jour/article/view/421icr micegc ratsoxys ratsembryo cryopreservationethylene glycolglycerolsucrose |
| spellingShingle | T. N. Igonina E. Yu. Brusentsev I. N. Rozhkova V. A. Naprimerov S. Ya. Amstislavsky A comparison of different cryoprotectant solutions and thawing methods for cryopreservation of embryos of mice and rats Вавиловский журнал генетики и селекции icr mice gc rats oxys rats embryo cryopreservation ethylene glycol glycerol sucrose |
| title | A comparison of different cryoprotectant solutions and thawing methods for cryopreservation of embryos of mice and rats |
| title_full | A comparison of different cryoprotectant solutions and thawing methods for cryopreservation of embryos of mice and rats |
| title_fullStr | A comparison of different cryoprotectant solutions and thawing methods for cryopreservation of embryos of mice and rats |
| title_full_unstemmed | A comparison of different cryoprotectant solutions and thawing methods for cryopreservation of embryos of mice and rats |
| title_short | A comparison of different cryoprotectant solutions and thawing methods for cryopreservation of embryos of mice and rats |
| title_sort | comparison of different cryoprotectant solutions and thawing methods for cryo preservation of embryos of mice and rats |
| topic | icr mice gc rats oxys rats embryo cryopreservation ethylene glycol glycerol sucrose |
| url | https://vavilov.elpub.ru/jour/article/view/421 |
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