Isolation, genotyping and antibiotic resistance analysis in Salmonella species isolated from turkey meat in Isfahan, Iran
Salmonella is one of the mainzoonotic bacteria in the poultry industry.The knowledge about biological characteristics and antibiotic resistance pattern can help medication in poultry and human. This research aimed to study Salmonella spp contamination and its antibiotic resistance in turkey meat in...
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Elsevier
2025-02-01
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| author | Farhad Lajmorak Seyedeh Ommolbanin Ghasemian Majid Gholami-Ahangaran |
| author_facet | Farhad Lajmorak Seyedeh Ommolbanin Ghasemian Majid Gholami-Ahangaran |
| author_sort | Farhad Lajmorak |
| collection | DOAJ |
| description | Salmonella is one of the mainzoonotic bacteria in the poultry industry.The knowledge about biological characteristics and antibiotic resistance pattern can help medication in poultry and human. This research aimed to study Salmonella spp contamination and its antibiotic resistance in turkey meat in Isfahan province, Iran.400 samples were collected from the turkey meat in slaughter line (May 2021 to May 2022). The conventional microbiological and biochemical tests were applied for isolation and typing of Salmonella spp. The polymerase chain reaction (PCR) was utilized for detection and typing of Salmonella strains. The antibiotic sensitivity test was achieved and all strains were evaluated for resistance genes of Act (3)-IV, Sul1 and qnrA. In microbiological examination, 32 Salmonella strains (8 %) were identified. All tested strains were positive for invA gene. By amplifying the FlicC and Prot6E genes, 28 and 4 strains had genes related to enteritidis and typhimurium, respectively. In disc diffusion test, the highest antibiotic resistance was to oxytetracycline (50 %) and the lowest was to gentamicin, amoxiclavulanic acid, cefotaxime and ceftriaxone. The results showed that 6 (18.75 %) and 10 (31.25 %) of the Salmonella spp were able to amplify Sul1 and qnrA genes, respectively. No Salmonella strain could amplify Act (3)-IV gene. 100 % of the strains carried the Sul1 and qnrA genes were resistant to sulfonamide, and enrofloxacin. Furthermore, 3 sulfonamide resistant strains (75 %) and 5 enrofloxacin resistant strains (83.33 %) were harbored Sul1 and qnrA genes, respectively. The prevalence and antibiotic resistance of Salmonella spp in turkey meat can induce health risk concern. However, the wide spectrum antibiotic resistance complicates the proper treatment of Salmonella infection in human. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj-art-69d270e4130e4fb1bbe1981e62a15dfd2025-01-30T05:14:39ZengElsevierHeliyon2405-84402025-02-01113e42220Isolation, genotyping and antibiotic resistance analysis in Salmonella species isolated from turkey meat in Isfahan, IranFarhad Lajmorak0Seyedeh Ommolbanin Ghasemian1Majid Gholami-Ahangaran2Graduated of Veterinary Department, Shoushtar Branch, Islamic Azad University, Shoushtar, IranDepartment of Veterinary, Behbahan Branch, Islamic Azad University, Behbahan, Iran; Corresponding author. Department of Veterinary Medicine, Behbahan Branch, Islamic Azad University, Behbahan, Iran.Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, IranSalmonella is one of the mainzoonotic bacteria in the poultry industry.The knowledge about biological characteristics and antibiotic resistance pattern can help medication in poultry and human. This research aimed to study Salmonella spp contamination and its antibiotic resistance in turkey meat in Isfahan province, Iran.400 samples were collected from the turkey meat in slaughter line (May 2021 to May 2022). The conventional microbiological and biochemical tests were applied for isolation and typing of Salmonella spp. The polymerase chain reaction (PCR) was utilized for detection and typing of Salmonella strains. The antibiotic sensitivity test was achieved and all strains were evaluated for resistance genes of Act (3)-IV, Sul1 and qnrA. In microbiological examination, 32 Salmonella strains (8 %) were identified. All tested strains were positive for invA gene. By amplifying the FlicC and Prot6E genes, 28 and 4 strains had genes related to enteritidis and typhimurium, respectively. In disc diffusion test, the highest antibiotic resistance was to oxytetracycline (50 %) and the lowest was to gentamicin, amoxiclavulanic acid, cefotaxime and ceftriaxone. The results showed that 6 (18.75 %) and 10 (31.25 %) of the Salmonella spp were able to amplify Sul1 and qnrA genes, respectively. No Salmonella strain could amplify Act (3)-IV gene. 100 % of the strains carried the Sul1 and qnrA genes were resistant to sulfonamide, and enrofloxacin. Furthermore, 3 sulfonamide resistant strains (75 %) and 5 enrofloxacin resistant strains (83.33 %) were harbored Sul1 and qnrA genes, respectively. The prevalence and antibiotic resistance of Salmonella spp in turkey meat can induce health risk concern. However, the wide spectrum antibiotic resistance complicates the proper treatment of Salmonella infection in human.http://www.sciencedirect.com/science/article/pii/S2405844025006000Antibiotic resistanceSalmonellaTurkey meatIsfahan |
| spellingShingle | Farhad Lajmorak Seyedeh Ommolbanin Ghasemian Majid Gholami-Ahangaran Isolation, genotyping and antibiotic resistance analysis in Salmonella species isolated from turkey meat in Isfahan, Iran Heliyon Antibiotic resistance Salmonella Turkey meat Isfahan |
| title | Isolation, genotyping and antibiotic resistance analysis in Salmonella species isolated from turkey meat in Isfahan, Iran |
| title_full | Isolation, genotyping and antibiotic resistance analysis in Salmonella species isolated from turkey meat in Isfahan, Iran |
| title_fullStr | Isolation, genotyping and antibiotic resistance analysis in Salmonella species isolated from turkey meat in Isfahan, Iran |
| title_full_unstemmed | Isolation, genotyping and antibiotic resistance analysis in Salmonella species isolated from turkey meat in Isfahan, Iran |
| title_short | Isolation, genotyping and antibiotic resistance analysis in Salmonella species isolated from turkey meat in Isfahan, Iran |
| title_sort | isolation genotyping and antibiotic resistance analysis in salmonella species isolated from turkey meat in isfahan iran |
| topic | Antibiotic resistance Salmonella Turkey meat Isfahan |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025006000 |
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