2′-Fucosyllactose as a prebiotic modulates the probiotic responses of Bifidobacterium bifidum
2′-Fucosyllactose (2′-FL), one of the most representative oligosaccharides in human milk, is intimately linked to the enrichment of specific Bifidobacterium species. However, the efficacy of 2′-FL in modulating the probiotic responses of bifidobacterium has been rarely researched. Thereinto, three k...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Current Research in Food Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2665927125000061 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832591773297278976 |
|---|---|
| author | Jingfang Du Hong Yang |
| author_facet | Jingfang Du Hong Yang |
| author_sort | Jingfang Du |
| collection | DOAJ |
| description | 2′-Fucosyllactose (2′-FL), one of the most representative oligosaccharides in human milk, is intimately linked to the enrichment of specific Bifidobacterium species. However, the efficacy of 2′-FL in modulating the probiotic responses of bifidobacterium has been rarely researched. Thereinto, three key issues have yet to be reported: the effects of 2′-FL hydrolysis on bifidobacterial growth, the protective effects of 2′-FL on bifidobacterium under gastrointestinal stress and the inhibitory activity of 2′-FL metabolites against Cronobacter spp. This work intended to address these concerns. 2′-FL dramatically accelerated the growth and proliferation of Bifidobacterium bifidum YH17 and Bifidobacterium bifidum BBI01. The glucose in lactose core on 2′-FL was preferable for B. bifidum to achieve substantial increases in biomass while the galactose was not readily available. Additionally, 2′-FL showed unique advantages in ameliorating the resistance of B. bifidum to gastrointestinal challenges. 2′-FL considerably improved the adhesive property of B. bifidum, thus facilitating the competitive elimination of Cronobacter sakazakii ATCC 29544 and Cronobacter muytjensii ATCC 51329 by B. bifidum. The growth inhibition of 2′-FL on the Cronobacter strains was mediated by promoting the secretion of antibacterial substances from B. bifidum. The inhibitory activity hinged on the B. bifidum strains. 2′-FL specifically induced B. bifidum BBI01 to produce some antibacterial substances that were proteinaceous, thermostable and relatively stable even at pH 8.0. These antibacterial substances played a key role in the inhibitory activity and had a synergistic effect with acidification. These observations provide a useful guideline for developing synbiotic supplements to intervene the infant gut microbiota. |
| format | Article |
| id | doaj-art-69cba5d188074ae798c9ddb9024aac76 |
| institution | Kabale University |
| issn | 2665-9271 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Food Science |
| spelling | doaj-art-69cba5d188074ae798c9ddb9024aac762025-01-22T05:44:02ZengElsevierCurrent Research in Food Science2665-92712025-01-01101009752′-Fucosyllactose as a prebiotic modulates the probiotic responses of Bifidobacterium bifidumJingfang Du0Hong Yang1State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, ChinaCorresponding author.; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China2′-Fucosyllactose (2′-FL), one of the most representative oligosaccharides in human milk, is intimately linked to the enrichment of specific Bifidobacterium species. However, the efficacy of 2′-FL in modulating the probiotic responses of bifidobacterium has been rarely researched. Thereinto, three key issues have yet to be reported: the effects of 2′-FL hydrolysis on bifidobacterial growth, the protective effects of 2′-FL on bifidobacterium under gastrointestinal stress and the inhibitory activity of 2′-FL metabolites against Cronobacter spp. This work intended to address these concerns. 2′-FL dramatically accelerated the growth and proliferation of Bifidobacterium bifidum YH17 and Bifidobacterium bifidum BBI01. The glucose in lactose core on 2′-FL was preferable for B. bifidum to achieve substantial increases in biomass while the galactose was not readily available. Additionally, 2′-FL showed unique advantages in ameliorating the resistance of B. bifidum to gastrointestinal challenges. 2′-FL considerably improved the adhesive property of B. bifidum, thus facilitating the competitive elimination of Cronobacter sakazakii ATCC 29544 and Cronobacter muytjensii ATCC 51329 by B. bifidum. The growth inhibition of 2′-FL on the Cronobacter strains was mediated by promoting the secretion of antibacterial substances from B. bifidum. The inhibitory activity hinged on the B. bifidum strains. 2′-FL specifically induced B. bifidum BBI01 to produce some antibacterial substances that were proteinaceous, thermostable and relatively stable even at pH 8.0. These antibacterial substances played a key role in the inhibitory activity and had a synergistic effect with acidification. These observations provide a useful guideline for developing synbiotic supplements to intervene the infant gut microbiota.http://www.sciencedirect.com/science/article/pii/S26659271250000612′-FucosyllactoseBifidobacterium bifidumGrowthGastrointestinalCronobacter |
| spellingShingle | Jingfang Du Hong Yang 2′-Fucosyllactose as a prebiotic modulates the probiotic responses of Bifidobacterium bifidum Current Research in Food Science 2′-Fucosyllactose Bifidobacterium bifidum Growth Gastrointestinal Cronobacter |
| title | 2′-Fucosyllactose as a prebiotic modulates the probiotic responses of Bifidobacterium bifidum |
| title_full | 2′-Fucosyllactose as a prebiotic modulates the probiotic responses of Bifidobacterium bifidum |
| title_fullStr | 2′-Fucosyllactose as a prebiotic modulates the probiotic responses of Bifidobacterium bifidum |
| title_full_unstemmed | 2′-Fucosyllactose as a prebiotic modulates the probiotic responses of Bifidobacterium bifidum |
| title_short | 2′-Fucosyllactose as a prebiotic modulates the probiotic responses of Bifidobacterium bifidum |
| title_sort | 2 fucosyllactose as a prebiotic modulates the probiotic responses of bifidobacterium bifidum |
| topic | 2′-Fucosyllactose Bifidobacterium bifidum Growth Gastrointestinal Cronobacter |
| url | http://www.sciencedirect.com/science/article/pii/S2665927125000061 |
| work_keys_str_mv | AT jingfangdu 2fucosyllactoseasaprebioticmodulatestheprobioticresponsesofbifidobacteriumbifidum AT hongyang 2fucosyllactoseasaprebioticmodulatestheprobioticresponsesofbifidobacteriumbifidum |