Overexpression of OsDUF868.12 enhances salt tolerance in rice
Excessive salt accumuln in soil is one of the most important abiotic stresses in agricultural environments. The Domain of Unknown Function 868 (DUF868) family, comprising 15 members in rice, has been identified in the protein family database. In this study, we cloned and functionally characterized O...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1458467/full |
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| author | Hao Chen Jiale Wan Jiali Zhu Ziyi Wang Caiyao Mao Wanjing Xu Juan Yang Yijuan Kong Xiaofei Zan Rongjun Chen Jianqing Zhu Zhengjun Xu Lihua Li Lihua Li |
| author_facet | Hao Chen Jiale Wan Jiali Zhu Ziyi Wang Caiyao Mao Wanjing Xu Juan Yang Yijuan Kong Xiaofei Zan Rongjun Chen Jianqing Zhu Zhengjun Xu Lihua Li Lihua Li |
| author_sort | Hao Chen |
| collection | DOAJ |
| description | Excessive salt accumuln in soil is one of the most important abiotic stresses in agricultural environments. The Domain of Unknown Function 868 (DUF868) family, comprising 15 members in rice, has been identified in the protein family database. In this study, we cloned and functionally characterized OsDUF868.12, a member of the OsDUF868 family, to elucidate its role in rice response to salt stress. A series of experiments, including RT-qPCR, Agrobacterium-mediated transient transformation in tobacco for localization analysis, phenotypic characterization, physiological and biochemical index measurement, and leaf staining, were conducted to investigate the function of OsDUF868.12 under salt stress. Transcriptional analysis revealed that OsDUF868.12 exhibited the most significant response to low temperature and salt stress. Preliminary subcellular localization studies indicated that OsDUF868.12 is localized in the cell membrane. Phenotypic Identification Experiments showed Overexpression lines of OsDUF868.12 enhanced resistance to salt stress and increased survival rates, while knockout lines of OsDUF868.12 were opposite. Physiological and biochemical assessments, along with leaf staining, demonstrated that overexpression of OsDUF868.12 improved the activity against oxidative stress.under salt stress. Furthermore, overexpression of OsDUF868.12 elevated the transcription levels of positively regulated salt stress-related genes. These findings suggest that overexpression of OsDUF868.12 enhances rice tolerance to salt stress at the molecular level through a series of regulatory mechanisms. This study provides valuable insights into the functional roles of the DUF868 family in plant responses to abiotic stress. |
| format | Article |
| id | doaj-art-699f2c4a8e1f4870bc25fb5d012b7966 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-699f2c4a8e1f4870bc25fb5d012b79662025-01-29T06:45:53ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011610.3389/fpls.2025.14584671458467Overexpression of OsDUF868.12 enhances salt tolerance in riceHao Chen0Jiale Wan1Jiali Zhu2Ziyi Wang3Caiyao Mao4Wanjing Xu5Juan Yang6Yijuan Kong7Xiaofei Zan8Rongjun Chen9Jianqing Zhu10Zhengjun Xu11Lihua Li12Lihua Li13State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, ChinaCrop Ecophysiology and Cultivation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaExcessive salt accumuln in soil is one of the most important abiotic stresses in agricultural environments. The Domain of Unknown Function 868 (DUF868) family, comprising 15 members in rice, has been identified in the protein family database. In this study, we cloned and functionally characterized OsDUF868.12, a member of the OsDUF868 family, to elucidate its role in rice response to salt stress. A series of experiments, including RT-qPCR, Agrobacterium-mediated transient transformation in tobacco for localization analysis, phenotypic characterization, physiological and biochemical index measurement, and leaf staining, were conducted to investigate the function of OsDUF868.12 under salt stress. Transcriptional analysis revealed that OsDUF868.12 exhibited the most significant response to low temperature and salt stress. Preliminary subcellular localization studies indicated that OsDUF868.12 is localized in the cell membrane. Phenotypic Identification Experiments showed Overexpression lines of OsDUF868.12 enhanced resistance to salt stress and increased survival rates, while knockout lines of OsDUF868.12 were opposite. Physiological and biochemical assessments, along with leaf staining, demonstrated that overexpression of OsDUF868.12 improved the activity against oxidative stress.under salt stress. Furthermore, overexpression of OsDUF868.12 elevated the transcription levels of positively regulated salt stress-related genes. These findings suggest that overexpression of OsDUF868.12 enhances rice tolerance to salt stress at the molecular level through a series of regulatory mechanisms. This study provides valuable insights into the functional roles of the DUF868 family in plant responses to abiotic stress.https://www.frontiersin.org/articles/10.3389/fpls.2025.1458467/fullOsDUF868.12riceabiotic stressesoxidative stressphysiological and biochemical index |
| spellingShingle | Hao Chen Jiale Wan Jiali Zhu Ziyi Wang Caiyao Mao Wanjing Xu Juan Yang Yijuan Kong Xiaofei Zan Rongjun Chen Jianqing Zhu Zhengjun Xu Lihua Li Lihua Li Overexpression of OsDUF868.12 enhances salt tolerance in rice Frontiers in Plant Science OsDUF868.12 rice abiotic stresses oxidative stress physiological and biochemical index |
| title | Overexpression of OsDUF868.12 enhances salt tolerance in rice |
| title_full | Overexpression of OsDUF868.12 enhances salt tolerance in rice |
| title_fullStr | Overexpression of OsDUF868.12 enhances salt tolerance in rice |
| title_full_unstemmed | Overexpression of OsDUF868.12 enhances salt tolerance in rice |
| title_short | Overexpression of OsDUF868.12 enhances salt tolerance in rice |
| title_sort | overexpression of osduf868 12 enhances salt tolerance in rice |
| topic | OsDUF868.12 rice abiotic stresses oxidative stress physiological and biochemical index |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1458467/full |
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