Intrafamilial Genotyping of Helicobacter pylori from Faecal DNA
Helicobacter pylori infection, often acquired in early childhood, is a global cause of undernutrition, gastritis, peptic ulcer disease and gastric carcinoma. This study tested the feasibility of using H. pylori shed in the faeces as a source of DNA for non-invasive epidemiological studies. H. pylori...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2011/491035 |
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| author | M. McMillan W. G. MacKay C. L. Williams A. J. Shepherd C. Malcolm L. T. Weaver |
| author_facet | M. McMillan W. G. MacKay C. L. Williams A. J. Shepherd C. Malcolm L. T. Weaver |
| author_sort | M. McMillan |
| collection | DOAJ |
| description | Helicobacter pylori infection, often acquired in early childhood, is a global cause of undernutrition, gastritis, peptic ulcer disease and gastric carcinoma. This study tested the feasibility of using H. pylori shed in the faeces as a source of DNA for non-invasive epidemiological studies. H. pylori DNA was chemically recovered and isolated using a specific biotinylated oligonucleotide probe with magnetic capture from 28 H. pylori positive faecal samples obtained from children attending hospital for the investigation of suspected H. pylori infection, together with close family members. Random amplification of polymorphic DNA (RAPD) was subsequently used to discriminate each isolate. 93% of stool samples selected were typeable. Parent, child and sibling samples were compared and similarities determined. Phylogenetic analysis showed that H. pylori DNA obtained from the faeces can be used to genotype individual strains, offering a means of studying intrafamilial transfer of this microorganism. |
| format | Article |
| id | doaj-art-6984bc8512424bffa4b64d5da7aaec41 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-6984bc8512424bffa4b64d5da7aaec412025-02-03T05:44:22ZengWileyGastroenterology Research and Practice1687-61211687-630X2011-01-01201110.1155/2011/491035491035Intrafamilial Genotyping of Helicobacter pylori from Faecal DNAM. McMillan0W. G. MacKay1C. L. Williams2A. J. Shepherd3C. Malcolm4L. T. Weaver5Child Health, School of Medicine, University of Glasgow, Glasgow G3 8SJ, UKChild Health, School of Medicine, University of Glasgow, Glasgow G3 8SJ, UKChild Health, School of Medicine, University of Glasgow, Glasgow G3 8SJ, UKDepartment of Nursing and Midwifery, University of Stirling, Stirling FK9 4LA, UKDepartment of Nursing and Midwifery, University of Stirling, Stirling FK9 4LA, UKChild Health, School of Medicine, University of Glasgow, Glasgow G3 8SJ, UKHelicobacter pylori infection, often acquired in early childhood, is a global cause of undernutrition, gastritis, peptic ulcer disease and gastric carcinoma. This study tested the feasibility of using H. pylori shed in the faeces as a source of DNA for non-invasive epidemiological studies. H. pylori DNA was chemically recovered and isolated using a specific biotinylated oligonucleotide probe with magnetic capture from 28 H. pylori positive faecal samples obtained from children attending hospital for the investigation of suspected H. pylori infection, together with close family members. Random amplification of polymorphic DNA (RAPD) was subsequently used to discriminate each isolate. 93% of stool samples selected were typeable. Parent, child and sibling samples were compared and similarities determined. Phylogenetic analysis showed that H. pylori DNA obtained from the faeces can be used to genotype individual strains, offering a means of studying intrafamilial transfer of this microorganism.http://dx.doi.org/10.1155/2011/491035 |
| spellingShingle | M. McMillan W. G. MacKay C. L. Williams A. J. Shepherd C. Malcolm L. T. Weaver Intrafamilial Genotyping of Helicobacter pylori from Faecal DNA Gastroenterology Research and Practice |
| title | Intrafamilial Genotyping of Helicobacter pylori from Faecal DNA |
| title_full | Intrafamilial Genotyping of Helicobacter pylori from Faecal DNA |
| title_fullStr | Intrafamilial Genotyping of Helicobacter pylori from Faecal DNA |
| title_full_unstemmed | Intrafamilial Genotyping of Helicobacter pylori from Faecal DNA |
| title_short | Intrafamilial Genotyping of Helicobacter pylori from Faecal DNA |
| title_sort | intrafamilial genotyping of helicobacter pylori from faecal dna |
| url | http://dx.doi.org/10.1155/2011/491035 |
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