Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2/TLR4 Signaling Pathway
Postoperative cognitive dysfunction (POCD) is a severe complication of cardiopulmonary bypass (CPB) and has common characteristics such as acute cognitive dysfunction, impaired memory, and inattention. Mesenchymal stem cells (MSCs) are multipotent cells that have therapeutic potentials mainly throug...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2020/2134565 |
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| author | Chun Yang Shengnan Sun Qi Zhang Jia Guo Tengfei Wu Ying Liu Min Yang Yan Zhang Yinghua Peng |
| author_facet | Chun Yang Shengnan Sun Qi Zhang Jia Guo Tengfei Wu Ying Liu Min Yang Yan Zhang Yinghua Peng |
| author_sort | Chun Yang |
| collection | DOAJ |
| description | Postoperative cognitive dysfunction (POCD) is a severe complication of cardiopulmonary bypass (CPB) and has common characteristics such as acute cognitive dysfunction, impaired memory, and inattention. Mesenchymal stem cells (MSCs) are multipotent cells that have therapeutic potentials mainly through paracrine action via secreting growth factors and cytokines. Exosomes are one of the important paracrine factors and have been reported as potential cell-free therapy for the treatment of autoimmune and central nervous system disorders. In this study, we examined exosomes derived from antler MSCs (AMSCs) of POCD rats after CPB and evaluated their potential regulatory mechanisms. AMSC-derived exosomes reduced neurological damage and brain damage and prevent apoptosis in CPB rats. Furthermore, AMSC-derived exosomes were found to reduce hippocampal neuronal apoptosis and the expression of TLR2, TLR4, MyD88, and NF-κB in CPB rats. However, the above effects of AMSC-derived exosomes on CPB rats were abolished partially by toll-like receptor 2/4 (TLR2/TLR4) agonist (LPS-EB). In conclusion, AMSC-derived exosomes can improve cognitive function in CPB rats through inhibiting the TLR2/TLR4 signaling pathway. |
| format | Article |
| id | doaj-art-69814955d9f242ec86ccb0243a6d523b |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-69814955d9f242ec86ccb0243a6d523b2025-02-03T01:05:07ZengWileyStem Cells International1687-966X1687-96782020-01-01202010.1155/2020/21345652134565Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2/TLR4 Signaling PathwayChun Yang0Shengnan Sun1Qi Zhang2Jia Guo3Tengfei Wu4Ying Liu5Min Yang6Yan Zhang7Yinghua Peng8Institute of Special Wild Economic Animal and Plants, Chinese Academy of Agricultural Sciences, Changchun, ChinaInstitute of Special Wild Economic Animal and Plants, Chinese Academy of Agricultural Sciences, Changchun, ChinaInstitute of Special Wild Economic Animal and Plants, Chinese Academy of Agricultural Sciences, Changchun, ChinaInstitute of Special Wild Economic Animal and Plants, Chinese Academy of Agricultural Sciences, Changchun, ChinaDepartment of Laboratory Animal Science, China Medical University, Shenyang, ChinaInstitute of Special Wild Economic Animal and Plants, Chinese Academy of Agricultural Sciences, Changchun, ChinaInstitute of Special Wild Economic Animal and Plants, Chinese Academy of Agricultural Sciences, Changchun, ChinaKey Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, ChinaInstitute of Special Wild Economic Animal and Plants, Chinese Academy of Agricultural Sciences, Changchun, ChinaPostoperative cognitive dysfunction (POCD) is a severe complication of cardiopulmonary bypass (CPB) and has common characteristics such as acute cognitive dysfunction, impaired memory, and inattention. Mesenchymal stem cells (MSCs) are multipotent cells that have therapeutic potentials mainly through paracrine action via secreting growth factors and cytokines. Exosomes are one of the important paracrine factors and have been reported as potential cell-free therapy for the treatment of autoimmune and central nervous system disorders. In this study, we examined exosomes derived from antler MSCs (AMSCs) of POCD rats after CPB and evaluated their potential regulatory mechanisms. AMSC-derived exosomes reduced neurological damage and brain damage and prevent apoptosis in CPB rats. Furthermore, AMSC-derived exosomes were found to reduce hippocampal neuronal apoptosis and the expression of TLR2, TLR4, MyD88, and NF-κB in CPB rats. However, the above effects of AMSC-derived exosomes on CPB rats were abolished partially by toll-like receptor 2/4 (TLR2/TLR4) agonist (LPS-EB). In conclusion, AMSC-derived exosomes can improve cognitive function in CPB rats through inhibiting the TLR2/TLR4 signaling pathway.http://dx.doi.org/10.1155/2020/2134565 |
| spellingShingle | Chun Yang Shengnan Sun Qi Zhang Jia Guo Tengfei Wu Ying Liu Min Yang Yan Zhang Yinghua Peng Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2/TLR4 Signaling Pathway Stem Cells International |
| title | Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2/TLR4 Signaling Pathway |
| title_full | Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2/TLR4 Signaling Pathway |
| title_fullStr | Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2/TLR4 Signaling Pathway |
| title_full_unstemmed | Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2/TLR4 Signaling Pathway |
| title_short | Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2/TLR4 Signaling Pathway |
| title_sort | exosomes of antler mesenchymal stem cells improve postoperative cognitive dysfunction in cardiopulmonary bypass rats through inhibiting the tlr2 tlr4 signaling pathway |
| url | http://dx.doi.org/10.1155/2020/2134565 |
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