Supplementation with yak (Bos grunniens) bone collagen hydrolysate altered the structure of gut microbiota and elevated short-chain fatty acid production in mice
In this study, yak bone collagen hydrolysate (YBCH) was produced by mixed proteases and provided to standard-diet mice at a different dose (low dose (LD), medium dose (MD), and high dose (HD)) to investigate its effects on the composition of gut microbiota and short-chain fatty acids (SCFA) producti...
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| Format: | Article |
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Tsinghua University Press
2023-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000174 |
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| author | Zitao Guo Dalong Yi Bo Hu Lingyu Zhu Ji Zhang Yuliang Yang Chunyu Liu Yi Shi Zhenghua Gu Yu Xin Huaigao Liu Liang Zhang |
| author_facet | Zitao Guo Dalong Yi Bo Hu Lingyu Zhu Ji Zhang Yuliang Yang Chunyu Liu Yi Shi Zhenghua Gu Yu Xin Huaigao Liu Liang Zhang |
| author_sort | Zitao Guo |
| collection | DOAJ |
| description | In this study, yak bone collagen hydrolysate (YBCH) was produced by mixed proteases and provided to standard-diet mice at a different dose (low dose (LD), medium dose (MD), and high dose (HD)) to investigate its effects on the composition of gut microbiota and short-chain fatty acids (SCFA) production. It was found that YBCH was mainly composed of small molecular peptides whose molecular weight below 2000 Da. Notably, supplementation with different doses of YBCH could significantly downregulate the ratio of Firmicutes to Bacteroidetes in the fecal microbiota. At the family level, the Lachnospiraceae abundance was significantly reduced in the YBCH gavage groups (mean reduction ratio 41.7 %, 35.2 %, and 36.4 % for LD, MD, and HD group, respectively). The predicted functions of gut microbes in the MD group were significantly increased at “lipid metabolism” and “glycan biosynthesis and metabolism”. Moreover, the SCFA production in the YBCH groups was elevated. Especially, the concentration of acetic acid, propionic acid, and butyric acid in the MD group was separately increased 79.7 %, 89.2 %, and 78.8 % than that in the NC group. These results indicated that YBCH might be applied in the development of functional food for intestinal microecological regulation. |
| format | Article |
| id | doaj-art-697faafadde94df98d6f9e71b49629a3 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-697faafadde94df98d6f9e71b49629a32025-02-03T05:40:25ZengTsinghua University PressFood Science and Human Wellness2213-45302023-09-0112516371645Supplementation with yak (Bos grunniens) bone collagen hydrolysate altered the structure of gut microbiota and elevated short-chain fatty acid production in miceZitao Guo0Dalong Yi1Bo Hu2Lingyu Zhu3Ji Zhang4Yuliang Yang5Chunyu Liu6Yi Shi7Zhenghua Gu8Yu Xin9Huaigao Liu10Liang Zhang11National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, ChinaAnhui Guotai Biotechnology Co., Ltd, Xuancheng 242100, ChinaNational Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, ChinaNational Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, ChinaNational Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, ChinaNational Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, ChinaNational Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, ChinaNational Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, ChinaNational Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, ChinaNational Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, ChinaAnhui Guotai Biotechnology Co., Ltd, Xuancheng 242100, ChinaNational Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, China; Corresponding author at: National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China.In this study, yak bone collagen hydrolysate (YBCH) was produced by mixed proteases and provided to standard-diet mice at a different dose (low dose (LD), medium dose (MD), and high dose (HD)) to investigate its effects on the composition of gut microbiota and short-chain fatty acids (SCFA) production. It was found that YBCH was mainly composed of small molecular peptides whose molecular weight below 2000 Da. Notably, supplementation with different doses of YBCH could significantly downregulate the ratio of Firmicutes to Bacteroidetes in the fecal microbiota. At the family level, the Lachnospiraceae abundance was significantly reduced in the YBCH gavage groups (mean reduction ratio 41.7 %, 35.2 %, and 36.4 % for LD, MD, and HD group, respectively). The predicted functions of gut microbes in the MD group were significantly increased at “lipid metabolism” and “glycan biosynthesis and metabolism”. Moreover, the SCFA production in the YBCH groups was elevated. Especially, the concentration of acetic acid, propionic acid, and butyric acid in the MD group was separately increased 79.7 %, 89.2 %, and 78.8 % than that in the NC group. These results indicated that YBCH might be applied in the development of functional food for intestinal microecological regulation.http://www.sciencedirect.com/science/article/pii/S2213453023000174Yak bone collagen hydrolysatePeptidesGut microbiotaShort-chain fatty acid;Lachnospiraceae |
| spellingShingle | Zitao Guo Dalong Yi Bo Hu Lingyu Zhu Ji Zhang Yuliang Yang Chunyu Liu Yi Shi Zhenghua Gu Yu Xin Huaigao Liu Liang Zhang Supplementation with yak (Bos grunniens) bone collagen hydrolysate altered the structure of gut microbiota and elevated short-chain fatty acid production in mice Food Science and Human Wellness Yak bone collagen hydrolysate Peptides Gut microbiota Short-chain fatty acid; Lachnospiraceae |
| title | Supplementation with yak (Bos grunniens) bone collagen hydrolysate altered the structure of gut microbiota and elevated short-chain fatty acid production in mice |
| title_full | Supplementation with yak (Bos grunniens) bone collagen hydrolysate altered the structure of gut microbiota and elevated short-chain fatty acid production in mice |
| title_fullStr | Supplementation with yak (Bos grunniens) bone collagen hydrolysate altered the structure of gut microbiota and elevated short-chain fatty acid production in mice |
| title_full_unstemmed | Supplementation with yak (Bos grunniens) bone collagen hydrolysate altered the structure of gut microbiota and elevated short-chain fatty acid production in mice |
| title_short | Supplementation with yak (Bos grunniens) bone collagen hydrolysate altered the structure of gut microbiota and elevated short-chain fatty acid production in mice |
| title_sort | supplementation with yak bos grunniens bone collagen hydrolysate altered the structure of gut microbiota and elevated short chain fatty acid production in mice |
| topic | Yak bone collagen hydrolysate Peptides Gut microbiota Short-chain fatty acid; Lachnospiraceae |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000174 |
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