Evaluation of the Starch Quantification Methods of Musa paradisiaca, Manihot esculenta, and Dioscorea trífida Using Factorial Experiments

Background. Starch and its products are used in a variety of ways for both the food and nonfood industries. A factorial experiment is carried out with two factors to explain the behavior of the percentage of starch, where the factors correspond to the extraction method and to the raw material. Metho...

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Main Authors: J. J. Lafont-Mendoza, C. A. Severiche-Sierra, J. Jaimes-Morales
Format: Article
Language:English
Published: Wiley 2018-01-01
Series:International Journal of Food Science
Online Access:http://dx.doi.org/10.1155/2018/5901930
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author J. J. Lafont-Mendoza
C. A. Severiche-Sierra
J. Jaimes-Morales
author_facet J. J. Lafont-Mendoza
C. A. Severiche-Sierra
J. Jaimes-Morales
author_sort J. J. Lafont-Mendoza
collection DOAJ
description Background. Starch and its products are used in a variety of ways for both the food and nonfood industries. A factorial experiment is carried out with two factors to explain the behavior of the percentage of starch, where the factors correspond to the extraction method and to the raw material. Method. Three methods were used in triplicate: the first followed the official technique of the Association of Official Analytical Chemists (AOAC), to perform acid hydrolysis and quantification of starch by Titulation; the second method involved the colorful reaction with iodine using the UV equipment to measure the absorbance and calculate the percentage of starch; as a third method the FTIR was used, through which the concentration of the starch was calculated by the area under the curve obtained from the spectrum. Results. there is an effect of both the method and the raw material on the percentage of starch, while there was no effect of the interaction; the Tukey test indicates that the highest average percentage of extraction occurs with the extraction method by Titulation and with the starch of Manihot esculenta. Conclusion. It is used as raw material. The method of quantification of starch by UV-VIS spectroscopy was the best for the study samples because it presented less deviation in relation to the FTIR and Titulation methods.
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spelling doaj-art-696d37267e8249e986467fc35f45a97f2025-02-03T07:24:22ZengWileyInternational Journal of Food Science2356-70152314-57652018-01-01201810.1155/2018/59019305901930Evaluation of the Starch Quantification Methods of Musa paradisiaca, Manihot esculenta, and Dioscorea trífida Using Factorial ExperimentsJ. J. Lafont-Mendoza0C. A. Severiche-Sierra1J. Jaimes-Morales2Universidad de Cordoba, Monteria, ColombiaUniversidad de Cartagena, Cartagena de Indias, ColombiaUniversidad de Cartagena, Cartagena de Indias, ColombiaBackground. Starch and its products are used in a variety of ways for both the food and nonfood industries. A factorial experiment is carried out with two factors to explain the behavior of the percentage of starch, where the factors correspond to the extraction method and to the raw material. Method. Three methods were used in triplicate: the first followed the official technique of the Association of Official Analytical Chemists (AOAC), to perform acid hydrolysis and quantification of starch by Titulation; the second method involved the colorful reaction with iodine using the UV equipment to measure the absorbance and calculate the percentage of starch; as a third method the FTIR was used, through which the concentration of the starch was calculated by the area under the curve obtained from the spectrum. Results. there is an effect of both the method and the raw material on the percentage of starch, while there was no effect of the interaction; the Tukey test indicates that the highest average percentage of extraction occurs with the extraction method by Titulation and with the starch of Manihot esculenta. Conclusion. It is used as raw material. The method of quantification of starch by UV-VIS spectroscopy was the best for the study samples because it presented less deviation in relation to the FTIR and Titulation methods.http://dx.doi.org/10.1155/2018/5901930
spellingShingle J. J. Lafont-Mendoza
C. A. Severiche-Sierra
J. Jaimes-Morales
Evaluation of the Starch Quantification Methods of Musa paradisiaca, Manihot esculenta, and Dioscorea trífida Using Factorial Experiments
International Journal of Food Science
title Evaluation of the Starch Quantification Methods of Musa paradisiaca, Manihot esculenta, and Dioscorea trífida Using Factorial Experiments
title_full Evaluation of the Starch Quantification Methods of Musa paradisiaca, Manihot esculenta, and Dioscorea trífida Using Factorial Experiments
title_fullStr Evaluation of the Starch Quantification Methods of Musa paradisiaca, Manihot esculenta, and Dioscorea trífida Using Factorial Experiments
title_full_unstemmed Evaluation of the Starch Quantification Methods of Musa paradisiaca, Manihot esculenta, and Dioscorea trífida Using Factorial Experiments
title_short Evaluation of the Starch Quantification Methods of Musa paradisiaca, Manihot esculenta, and Dioscorea trífida Using Factorial Experiments
title_sort evaluation of the starch quantification methods of musa paradisiaca manihot esculenta and dioscorea trifida using factorial experiments
url http://dx.doi.org/10.1155/2018/5901930
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