Evaluating passive immunity in piglets from sows vaccinated with a PEDV S protein subunit vaccine
Porcine epidemic diarrhea virus (PEDV) is a highly contagious virus that causes severe diarrhea and high mortality in neonatal piglets. Current control measures, such as inactivated and live-attenuated vaccines, have limitations in providing complete protection. In this study, we evaluate the immuno...
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| Format: | Article |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1498610/full |
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| author | Jiajia Liu Jiajia Liu Guangli Hu Shengjin Liu Guangcai Ren Liguo Gao Liguo Gao Zhiqing Zhao Rui Geng Dingli Wang Xiao Shen Feng Chen Hanqin Shen Hanqin Shen |
| author_facet | Jiajia Liu Jiajia Liu Guangli Hu Shengjin Liu Guangcai Ren Liguo Gao Liguo Gao Zhiqing Zhao Rui Geng Dingli Wang Xiao Shen Feng Chen Hanqin Shen Hanqin Shen |
| author_sort | Jiajia Liu |
| collection | DOAJ |
| description | Porcine epidemic diarrhea virus (PEDV) is a highly contagious virus that causes severe diarrhea and high mortality in neonatal piglets. Current control measures, such as inactivated and live-attenuated vaccines, have limitations in providing complete protection. In this study, we evaluate the immunogenicity and protective efficacy of a PEDV S protein subunit vaccine compared to a traditional inactivated vaccine. Piglets and Sows were immunized with either the subunit vaccine or an inactivated vaccine, and serum samples were collected to assess IgG and neutralizing antibody levels. Results demonstrated that the S protein subunit vaccine induced significantly higher IgG and neutralizing antibody levels in both piglets and sows compared to the inactivated vaccine. Piglets born to immunized sows were challenged with a virulent PEDV strain. Piglets from the subunit vaccine group exhibited lower viral shedding, reduced clinical symptoms, and minimal intestinal lesions. These findings suggest that the PEDV S protein subunit vaccine provides enhanced immunity and protection against PEDV, making it a promising candidate for preventing PEDV infections in swine. |
| format | Article |
| id | doaj-art-696aae6dcd7c448cad11318acbd5b3c3 |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-696aae6dcd7c448cad11318acbd5b3c32025-01-31T06:40:00ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-01-011410.3389/fcimb.2024.14986101498610Evaluating passive immunity in piglets from sows vaccinated with a PEDV S protein subunit vaccineJiajia Liu0Jiajia Liu1Guangli Hu2Shengjin Liu3Guangcai Ren4Liguo Gao5Liguo Gao6Zhiqing Zhao7Rui Geng8Dingli Wang9Xiao Shen10Feng Chen11Hanqin Shen12Hanqin Shen13Guangdong Enterprise Key Laboratory for Animal Health and Environmental Control, Wen’s Foodstuff Group Co. Ltd, Yunfu, ChinaYunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Yunfu, ChinaState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaCanton Biologics Co., Ltd, Guangzhou, ChinaGuangdong Enterprise Key Laboratory for Animal Health and Environmental Control, Wen’s Foodstuff Group Co. Ltd, Yunfu, ChinaYunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Yunfu, ChinaState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, ChinaState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, ChinaCanton Biologics Co., Ltd, Guangzhou, ChinaCanton Biologics Co., Ltd, Guangzhou, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaGuangdong Enterprise Key Laboratory for Animal Health and Environmental Control, Wen’s Foodstuff Group Co. Ltd, Yunfu, ChinaYunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Yunfu, ChinaPorcine epidemic diarrhea virus (PEDV) is a highly contagious virus that causes severe diarrhea and high mortality in neonatal piglets. Current control measures, such as inactivated and live-attenuated vaccines, have limitations in providing complete protection. In this study, we evaluate the immunogenicity and protective efficacy of a PEDV S protein subunit vaccine compared to a traditional inactivated vaccine. Piglets and Sows were immunized with either the subunit vaccine or an inactivated vaccine, and serum samples were collected to assess IgG and neutralizing antibody levels. Results demonstrated that the S protein subunit vaccine induced significantly higher IgG and neutralizing antibody levels in both piglets and sows compared to the inactivated vaccine. Piglets born to immunized sows were challenged with a virulent PEDV strain. Piglets from the subunit vaccine group exhibited lower viral shedding, reduced clinical symptoms, and minimal intestinal lesions. These findings suggest that the PEDV S protein subunit vaccine provides enhanced immunity and protection against PEDV, making it a promising candidate for preventing PEDV infections in swine.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1498610/fullporcine epidemic diarrhea virusPEDVspike proteinimmunogenicitysubunit vaccine |
| spellingShingle | Jiajia Liu Jiajia Liu Guangli Hu Shengjin Liu Guangcai Ren Liguo Gao Liguo Gao Zhiqing Zhao Rui Geng Dingli Wang Xiao Shen Feng Chen Hanqin Shen Hanqin Shen Evaluating passive immunity in piglets from sows vaccinated with a PEDV S protein subunit vaccine Frontiers in Cellular and Infection Microbiology porcine epidemic diarrhea virus PEDV spike protein immunogenicity subunit vaccine |
| title | Evaluating passive immunity in piglets from sows vaccinated with a PEDV S protein subunit vaccine |
| title_full | Evaluating passive immunity in piglets from sows vaccinated with a PEDV S protein subunit vaccine |
| title_fullStr | Evaluating passive immunity in piglets from sows vaccinated with a PEDV S protein subunit vaccine |
| title_full_unstemmed | Evaluating passive immunity in piglets from sows vaccinated with a PEDV S protein subunit vaccine |
| title_short | Evaluating passive immunity in piglets from sows vaccinated with a PEDV S protein subunit vaccine |
| title_sort | evaluating passive immunity in piglets from sows vaccinated with a pedv s protein subunit vaccine |
| topic | porcine epidemic diarrhea virus PEDV spike protein immunogenicity subunit vaccine |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1498610/full |
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