The Evidence of Cooperative Binding of a Ligand to G4 DNA
Intrinsic constants of the ligand binding with G4 DNA (guanine-rich DNA sequence) using quantitative standards can be convenient providing the assessment for elucidating the possibility of such structures participation in biochemical processes. In the present communication, the hard + soft modelling...
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Wiley
2017-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2017/6780521 |
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| author | A. G. Kudrev |
| author_facet | A. G. Kudrev |
| author_sort | A. G. Kudrev |
| collection | DOAJ |
| description | Intrinsic constants of the ligand binding with G4 DNA (guanine-rich DNA sequence) using quantitative standards can be convenient providing the assessment for elucidating the possibility of such structures participation in biochemical processes. In the present communication, the hard + soft modelling approach to calculate intrinsic constants of a ligand binding with short DNA molecule, particularly such as G4 DNA, has been proposed. The suggested approach has focused upon the quantitative evaluating of a mutual influence between sites and between bound ligands. The cross-validation between a new hard + soft modelling and conventional stepwise complex formation algorithm has been conducted. A number of simulated examples will illustrate the methodology. The experimental mole-ratio titration of TMPyP4 by G4 DNA [(CG3)2CGC(AG3)2G] has been reexamined. The [(CG3)2CGC(AG3)2G] that folds from a G-rich sequence found in the promoter region of c-kit oncogene can be considered as a molecule with two equivalent mutually influence binding sites. |
| format | Article |
| id | doaj-art-692e0debc8ca4d2f9b5cc33e9c96f599 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-692e0debc8ca4d2f9b5cc33e9c96f5992025-02-03T06:44:25ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732017-01-01201710.1155/2017/67805216780521The Evidence of Cooperative Binding of a Ligand to G4 DNAA. G. Kudrev0Saint Petersburg State University, 7/9 Universitetskaya Nab., St. Petersburg 199034, RussiaIntrinsic constants of the ligand binding with G4 DNA (guanine-rich DNA sequence) using quantitative standards can be convenient providing the assessment for elucidating the possibility of such structures participation in biochemical processes. In the present communication, the hard + soft modelling approach to calculate intrinsic constants of a ligand binding with short DNA molecule, particularly such as G4 DNA, has been proposed. The suggested approach has focused upon the quantitative evaluating of a mutual influence between sites and between bound ligands. The cross-validation between a new hard + soft modelling and conventional stepwise complex formation algorithm has been conducted. A number of simulated examples will illustrate the methodology. The experimental mole-ratio titration of TMPyP4 by G4 DNA [(CG3)2CGC(AG3)2G] has been reexamined. The [(CG3)2CGC(AG3)2G] that folds from a G-rich sequence found in the promoter region of c-kit oncogene can be considered as a molecule with two equivalent mutually influence binding sites.http://dx.doi.org/10.1155/2017/6780521 |
| spellingShingle | A. G. Kudrev The Evidence of Cooperative Binding of a Ligand to G4 DNA Journal of Analytical Methods in Chemistry |
| title | The Evidence of Cooperative Binding of a Ligand to G4 DNA |
| title_full | The Evidence of Cooperative Binding of a Ligand to G4 DNA |
| title_fullStr | The Evidence of Cooperative Binding of a Ligand to G4 DNA |
| title_full_unstemmed | The Evidence of Cooperative Binding of a Ligand to G4 DNA |
| title_short | The Evidence of Cooperative Binding of a Ligand to G4 DNA |
| title_sort | evidence of cooperative binding of a ligand to g4 dna |
| url | http://dx.doi.org/10.1155/2017/6780521 |
| work_keys_str_mv | AT agkudrev theevidenceofcooperativebindingofaligandtog4dna AT agkudrev evidenceofcooperativebindingofaligandtog4dna |