Novel soybean peptide iglycin ameliorates insulin resistance of high-fat diet fed C57BL/6J mice and differentiated 3T3L1 adipocytes with improvement of insulin signaling and mitochondrial function
Soy consumption has been associated with potential health benefits in reducing chronic diseases. These physiological functions have been attributed to soy proteins or more commonly to bioactive peptides. Thus, more studies are required to identify these bioactive peptides, and elucidate their biolog...
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Tsinghua University Press
2022-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022000970 |
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| author | Yinghuan Wu Ran Zhao Minxia Li Huiyun Li Zhengwang Chen Yanying Zhao |
| author_facet | Yinghuan Wu Ran Zhao Minxia Li Huiyun Li Zhengwang Chen Yanying Zhao |
| author_sort | Yinghuan Wu |
| collection | DOAJ |
| description | Soy consumption has been associated with potential health benefits in reducing chronic diseases. These physiological functions have been attributed to soy proteins or more commonly to bioactive peptides. Thus, more studies are required to identify these bioactive peptides, and elucidate their biological mechanisms of action. In the present study, a novel peptide iglycin was purified from soybean seeds with a molecular mass of 3.88 kDa. Thereafter, iglycin reduced fasting blood glucose and restored insulin sensitivity of C57BL/6J mice on a high-fat diet with increased phosphorylation of insulin receptor substrate 1 (IRS1) and AKT in adipose tissue. Furthermore, it improved glucose uptake, induced translocation of intracellular GLUT4 to plasma membrane and activation of insulin signaling in adipocytes under insulin-resistant condition. In addition, it decreased reactive oxygen species production, lipid peroxidation and inhibited adipocyte apoptosis with improved mitochondrial function as evidenced by up-regulation of succinate dehydrogenase activity, mitochondrial membrane potential and intracellular ATP store. These data suggested that iglycin ameliorated insulin resistance via activation of insulin signaling, which was associated with inhibition of oxidative stress, adipocyte apoptosis, and improvement of mitochondrial function. |
| format | Article |
| id | doaj-art-692b07c773234d718cf688f9c5728603 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-692b07c773234d718cf688f9c57286032025-02-02T23:24:56ZengTsinghua University PressFood Science and Human Wellness2213-45302022-11-0111615651572Novel soybean peptide iglycin ameliorates insulin resistance of high-fat diet fed C57BL/6J mice and differentiated 3T3L1 adipocytes with improvement of insulin signaling and mitochondrial functionYinghuan Wu0Ran Zhao1Minxia Li2Huiyun Li3Zhengwang Chen4Yanying Zhao5Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Ministry of Education, Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Sichuan Province, College of Life Science and Technology, Southwest Minzu University, Chengdu 610041, ChinaShandong Tianheng Inspection Co., Ltd., Heze 274000, ChinaShandong Tianheng Inspection Co., Ltd., Heze 274000, ChinaShandong Tianheng Inspection Co., Ltd., Heze 274000, ChinaKey Laboratory of Molecular Biophysics of Ministry of Education, School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Ministry of Education, Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Sichuan Province, College of Life Science and Technology, Southwest Minzu University, Chengdu 610041, China; Corresponding author at: College of Life Science and Technology, Southwest Minzu University, Chengdu 610041, China. Fax: +86-28-85522310.Soy consumption has been associated with potential health benefits in reducing chronic diseases. These physiological functions have been attributed to soy proteins or more commonly to bioactive peptides. Thus, more studies are required to identify these bioactive peptides, and elucidate their biological mechanisms of action. In the present study, a novel peptide iglycin was purified from soybean seeds with a molecular mass of 3.88 kDa. Thereafter, iglycin reduced fasting blood glucose and restored insulin sensitivity of C57BL/6J mice on a high-fat diet with increased phosphorylation of insulin receptor substrate 1 (IRS1) and AKT in adipose tissue. Furthermore, it improved glucose uptake, induced translocation of intracellular GLUT4 to plasma membrane and activation of insulin signaling in adipocytes under insulin-resistant condition. In addition, it decreased reactive oxygen species production, lipid peroxidation and inhibited adipocyte apoptosis with improved mitochondrial function as evidenced by up-regulation of succinate dehydrogenase activity, mitochondrial membrane potential and intracellular ATP store. These data suggested that iglycin ameliorated insulin resistance via activation of insulin signaling, which was associated with inhibition of oxidative stress, adipocyte apoptosis, and improvement of mitochondrial function.http://www.sciencedirect.com/science/article/pii/S2213453022000970Soybean peptideIglycinInsulin resistanceInsulin signaling |
| spellingShingle | Yinghuan Wu Ran Zhao Minxia Li Huiyun Li Zhengwang Chen Yanying Zhao Novel soybean peptide iglycin ameliorates insulin resistance of high-fat diet fed C57BL/6J mice and differentiated 3T3L1 adipocytes with improvement of insulin signaling and mitochondrial function Food Science and Human Wellness Soybean peptide Iglycin Insulin resistance Insulin signaling |
| title | Novel soybean peptide iglycin ameliorates insulin resistance of high-fat diet fed C57BL/6J mice and differentiated 3T3L1 adipocytes with improvement of insulin signaling and mitochondrial function |
| title_full | Novel soybean peptide iglycin ameliorates insulin resistance of high-fat diet fed C57BL/6J mice and differentiated 3T3L1 adipocytes with improvement of insulin signaling and mitochondrial function |
| title_fullStr | Novel soybean peptide iglycin ameliorates insulin resistance of high-fat diet fed C57BL/6J mice and differentiated 3T3L1 adipocytes with improvement of insulin signaling and mitochondrial function |
| title_full_unstemmed | Novel soybean peptide iglycin ameliorates insulin resistance of high-fat diet fed C57BL/6J mice and differentiated 3T3L1 adipocytes with improvement of insulin signaling and mitochondrial function |
| title_short | Novel soybean peptide iglycin ameliorates insulin resistance of high-fat diet fed C57BL/6J mice and differentiated 3T3L1 adipocytes with improvement of insulin signaling and mitochondrial function |
| title_sort | novel soybean peptide iglycin ameliorates insulin resistance of high fat diet fed c57bl 6j mice and differentiated 3t3l1 adipocytes with improvement of insulin signaling and mitochondrial function |
| topic | Soybean peptide Iglycin Insulin resistance Insulin signaling |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022000970 |
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