Dose- and sex-dependent effects on umbilical cord-derived mesenchymal stem cell efficacy in regeneration of a full-thickness tendon defect in a rat model
Background Mesenchymal stem cells (MSCs) have shown potential in regenerative medicine. In the present study the effects of MSC dosage and recipient sex on tendon regeneration were evaluated. Methods A full-thickness tendon defect (FTTD) was created on supraspinatus tendons (SSTs) of rats and cryopr...
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Korean Shoulder and Elbow Society
2025-02-01
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| Series: | Clinics in Shoulder and Elbow |
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| Online Access: | http://cisejournal.org/upload/pdf/cise-2024-00626.pdf |
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| author | Ji-Hye Yea Chris Hyunchul Jo |
| author_facet | Ji-Hye Yea Chris Hyunchul Jo |
| author_sort | Ji-Hye Yea |
| collection | DOAJ |
| description | Background Mesenchymal stem cells (MSCs) have shown potential in regenerative medicine. In the present study the effects of MSC dosage and recipient sex on tendon regeneration were evaluated. Methods A full-thickness tendon defect (FTTD) was created on supraspinatus tendons (SSTs) of rats and cryoprotective solution (CPS) and MSCs (0.05, 0.1 and 0.5 million MSCs [M-MSC] for female groups and 1.0 M-MSC for both female and male groups) were applied. After 2 and 4 weeks, macroscopic and histological evaluations were performed. Results Total macroscopic scores were improved in all MSC groups compared with the CPS group, with no significant differences among the MSC groups. Furthermore, all MSC groups had lower total degenerative scores than the CPS group; however, only 0.1 M-MSC, 0.5 M-MSC, and 1 M-MSC groups showed significantly improved hyalinization compared with the CPS group at 4 weeks. Collagen organization and coherence were higher in all MSC groups than in the CPS group at both 2 and 4 weeks; however, 0.5 M-MSC and 1 M-MSC groups scored better than the 0.05 M-MSC group at 4 weeks. Heterotopic matrix analysis revealed smaller glycosaminoglycan (GAG)-rich areas in the 0.1 M-MSC, 0.5 M-MSC, and 1 M-MSC groups compared with the CPS group at 4 weeks. Overall, macroscopic and histological evaluations were not significantly different between female and male groups except for GAG-rich area. Conclusions The MSC dosage affected collagen and heterotopic matrix formation in a FTTD rat model; however, the efficacy of MSCs (1.0 M dose) in collagen regeneration was not affected based on the sex of the recipient. Level of evidence I. |
| format | Article |
| id | doaj-art-68e8d9dbff5643dc862e4e3aea52dff5 |
| institution | OA Journals |
| issn | 2288-8721 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Korean Shoulder and Elbow Society |
| record_format | Article |
| series | Clinics in Shoulder and Elbow |
| spelling | doaj-art-68e8d9dbff5643dc862e4e3aea52dff52025-08-20T02:32:15ZengKorean Shoulder and Elbow SocietyClinics in Shoulder and Elbow2288-87212025-02-01281495910.5397/cise.2024.006261024Dose- and sex-dependent effects on umbilical cord-derived mesenchymal stem cell efficacy in regeneration of a full-thickness tendon defect in a rat modelJi-Hye Yea0Chris Hyunchul Jo1 Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA Department of Translational Medicine, Seoul National University College of Medicine, Seoul, KoreaBackground Mesenchymal stem cells (MSCs) have shown potential in regenerative medicine. In the present study the effects of MSC dosage and recipient sex on tendon regeneration were evaluated. Methods A full-thickness tendon defect (FTTD) was created on supraspinatus tendons (SSTs) of rats and cryoprotective solution (CPS) and MSCs (0.05, 0.1 and 0.5 million MSCs [M-MSC] for female groups and 1.0 M-MSC for both female and male groups) were applied. After 2 and 4 weeks, macroscopic and histological evaluations were performed. Results Total macroscopic scores were improved in all MSC groups compared with the CPS group, with no significant differences among the MSC groups. Furthermore, all MSC groups had lower total degenerative scores than the CPS group; however, only 0.1 M-MSC, 0.5 M-MSC, and 1 M-MSC groups showed significantly improved hyalinization compared with the CPS group at 4 weeks. Collagen organization and coherence were higher in all MSC groups than in the CPS group at both 2 and 4 weeks; however, 0.5 M-MSC and 1 M-MSC groups scored better than the 0.05 M-MSC group at 4 weeks. Heterotopic matrix analysis revealed smaller glycosaminoglycan (GAG)-rich areas in the 0.1 M-MSC, 0.5 M-MSC, and 1 M-MSC groups compared with the CPS group at 4 weeks. Overall, macroscopic and histological evaluations were not significantly different between female and male groups except for GAG-rich area. Conclusions The MSC dosage affected collagen and heterotopic matrix formation in a FTTD rat model; however, the efficacy of MSCs (1.0 M dose) in collagen regeneration was not affected based on the sex of the recipient. Level of evidence I.http://cisejournal.org/upload/pdf/cise-2024-00626.pdfshoulder paintendon regenerationmesenchymal stem cellssexdose |
| spellingShingle | Ji-Hye Yea Chris Hyunchul Jo Dose- and sex-dependent effects on umbilical cord-derived mesenchymal stem cell efficacy in regeneration of a full-thickness tendon defect in a rat model Clinics in Shoulder and Elbow shoulder pain tendon regeneration mesenchymal stem cells sex dose |
| title | Dose- and sex-dependent effects on umbilical cord-derived mesenchymal stem cell efficacy in regeneration of a full-thickness tendon defect in a rat model |
| title_full | Dose- and sex-dependent effects on umbilical cord-derived mesenchymal stem cell efficacy in regeneration of a full-thickness tendon defect in a rat model |
| title_fullStr | Dose- and sex-dependent effects on umbilical cord-derived mesenchymal stem cell efficacy in regeneration of a full-thickness tendon defect in a rat model |
| title_full_unstemmed | Dose- and sex-dependent effects on umbilical cord-derived mesenchymal stem cell efficacy in regeneration of a full-thickness tendon defect in a rat model |
| title_short | Dose- and sex-dependent effects on umbilical cord-derived mesenchymal stem cell efficacy in regeneration of a full-thickness tendon defect in a rat model |
| title_sort | dose and sex dependent effects on umbilical cord derived mesenchymal stem cell efficacy in regeneration of a full thickness tendon defect in a rat model |
| topic | shoulder pain tendon regeneration mesenchymal stem cells sex dose |
| url | http://cisejournal.org/upload/pdf/cise-2024-00626.pdf |
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