A forward-design approach to increase the production of poly-3-hydroxybutyrate in genetically engineered Escherichia coli.
Biopolymers, such as poly-3-hydroxybutyrate (P(3HB)) are produced as a carbon store in an array of organisms and exhibit characteristics which are similar to oil-derived plastics, yet have the added advantages of biodegradability and biocompatibility. Despite these advantages, P(3HB) production is c...
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117202&type=printable |
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| author | Richard Kelwick Margarita Kopniczky Iain Bower Wenqiang Chi Matthew Ho Wai Chin Sisi Fan Jemma Pilcher James Strutt Alexander J Webb Kirsten Jensen Guy-Bart Stan Richard Kitney Paul Freemont |
| author_facet | Richard Kelwick Margarita Kopniczky Iain Bower Wenqiang Chi Matthew Ho Wai Chin Sisi Fan Jemma Pilcher James Strutt Alexander J Webb Kirsten Jensen Guy-Bart Stan Richard Kitney Paul Freemont |
| author_sort | Richard Kelwick |
| collection | DOAJ |
| description | Biopolymers, such as poly-3-hydroxybutyrate (P(3HB)) are produced as a carbon store in an array of organisms and exhibit characteristics which are similar to oil-derived plastics, yet have the added advantages of biodegradability and biocompatibility. Despite these advantages, P(3HB) production is currently more expensive than the production of oil-derived plastics, and therefore, more efficient P(3HB) production processes would be desirable. In this study, we describe the model-guided design and experimental validation of several engineered P(3HB) producing operons. In particular, we describe the characterization of a hybrid phaCAB operon that consists of a dual promoter (native and J23104) and RBS (native and B0034) design. P(3HB) production at 24 h was around six-fold higher in hybrid phaCAB engineered Escherichia coli in comparison to E. coli engineered with the native phaCAB operon from Ralstonia eutropha H16. Additionally, we describe the utilization of non-recyclable waste as a low-cost carbon source for the production of P(3HB). |
| format | Article |
| id | doaj-art-68d30e1d6daf4b51b752ccfb5c8e7c79 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-68d30e1d6daf4b51b752ccfb5c8e7c792025-08-20T03:01:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01102e011720210.1371/journal.pone.0117202A forward-design approach to increase the production of poly-3-hydroxybutyrate in genetically engineered Escherichia coli.Richard KelwickMargarita KopniczkyIain BowerWenqiang ChiMatthew Ho Wai ChinSisi FanJemma PilcherJames StruttAlexander J WebbKirsten JensenGuy-Bart StanRichard KitneyPaul FreemontBiopolymers, such as poly-3-hydroxybutyrate (P(3HB)) are produced as a carbon store in an array of organisms and exhibit characteristics which are similar to oil-derived plastics, yet have the added advantages of biodegradability and biocompatibility. Despite these advantages, P(3HB) production is currently more expensive than the production of oil-derived plastics, and therefore, more efficient P(3HB) production processes would be desirable. In this study, we describe the model-guided design and experimental validation of several engineered P(3HB) producing operons. In particular, we describe the characterization of a hybrid phaCAB operon that consists of a dual promoter (native and J23104) and RBS (native and B0034) design. P(3HB) production at 24 h was around six-fold higher in hybrid phaCAB engineered Escherichia coli in comparison to E. coli engineered with the native phaCAB operon from Ralstonia eutropha H16. Additionally, we describe the utilization of non-recyclable waste as a low-cost carbon source for the production of P(3HB).https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117202&type=printable |
| spellingShingle | Richard Kelwick Margarita Kopniczky Iain Bower Wenqiang Chi Matthew Ho Wai Chin Sisi Fan Jemma Pilcher James Strutt Alexander J Webb Kirsten Jensen Guy-Bart Stan Richard Kitney Paul Freemont A forward-design approach to increase the production of poly-3-hydroxybutyrate in genetically engineered Escherichia coli. PLoS ONE |
| title | A forward-design approach to increase the production of poly-3-hydroxybutyrate in genetically engineered Escherichia coli. |
| title_full | A forward-design approach to increase the production of poly-3-hydroxybutyrate in genetically engineered Escherichia coli. |
| title_fullStr | A forward-design approach to increase the production of poly-3-hydroxybutyrate in genetically engineered Escherichia coli. |
| title_full_unstemmed | A forward-design approach to increase the production of poly-3-hydroxybutyrate in genetically engineered Escherichia coli. |
| title_short | A forward-design approach to increase the production of poly-3-hydroxybutyrate in genetically engineered Escherichia coli. |
| title_sort | forward design approach to increase the production of poly 3 hydroxybutyrate in genetically engineered escherichia coli |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117202&type=printable |
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