Contribution to diagnostics/prognostics of tuberculosis in children. I. New methods of assaying zinc and simultaneously copper and zinc in diluted sera by flame atomic-absorption spectrometry
In an attempt to provide a reliable status of metal ions in children, new methods of analysis of children’s sera are proposed. New flame atomic-absorption spectrometric (FAAS) methods are simple, cost- and time-effective and, above all, labor-, reagent- and sample-saving. Two methods were suggested:...
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| Format: | Article |
| Language: | English |
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Sciendo
2015-09-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.1515/acph-2015-0026 |
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| author | Luterotti Svjetlana Kordić Tončica Vukman Dodig Slavica |
| author_facet | Luterotti Svjetlana Kordić Tončica Vukman Dodig Slavica |
| author_sort | Luterotti Svjetlana |
| collection | DOAJ |
| description | In an attempt to provide a reliable status of metal ions in children, new methods of analysis of children’s sera are proposed. New flame atomic-absorption spectrometric (FAAS) methods are simple, cost- and time-effective and, above all, labor-, reagent- and sample-saving. Two methods were suggested: method A for simultaneous determination of Cu and Zn from 5-fold diluted sera, and method B, for assaying zinc alone in 10-fold diluted samples. Both methods are based on a single-step sample pretreatment (deproteinization with 3 mol dm–3 HCl). Method A uses a single-step calibration with a mixed standard. The main advantage of method B is an additional reduction in sample consumption. Both methods were fully validated against reference methods. Accuracy, sensitivity and precision have proven them to be comparable to the reference methods in terms of analytical performance, and applicable to analyses of children’s sera. |
| format | Article |
| id | doaj-art-68d14cc67bb2418db86fd3cbe034864e |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2015-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-68d14cc67bb2418db86fd3cbe034864e2025-02-02T11:55:54ZengSciendoActa Pharmaceutica1846-95582015-09-0165331132010.1515/acph-2015-0026acph-2015-0026Contribution to diagnostics/prognostics of tuberculosis in children. I. New methods of assaying zinc and simultaneously copper and zinc in diluted sera by flame atomic-absorption spectrometryLuterotti Svjetlana0Kordić Tončica Vukman1Dodig Slavica2 Faculty of Pharmacy and Biochemistry, University of Zagreb HR-10000 Zagreb, Croatia Agency for Medicinal Products and Medical Devices HR-10000 Zagreb, Croatia Srebrnjak Children’s Hospital, HR-10000 Zagreb, CroatiaIn an attempt to provide a reliable status of metal ions in children, new methods of analysis of children’s sera are proposed. New flame atomic-absorption spectrometric (FAAS) methods are simple, cost- and time-effective and, above all, labor-, reagent- and sample-saving. Two methods were suggested: method A for simultaneous determination of Cu and Zn from 5-fold diluted sera, and method B, for assaying zinc alone in 10-fold diluted samples. Both methods are based on a single-step sample pretreatment (deproteinization with 3 mol dm–3 HCl). Method A uses a single-step calibration with a mixed standard. The main advantage of method B is an additional reduction in sample consumption. Both methods were fully validated against reference methods. Accuracy, sensitivity and precision have proven them to be comparable to the reference methods in terms of analytical performance, and applicable to analyses of children’s sera.https://doi.org/10.1515/acph-2015-0026copperzincflame atomic-absorption spectrometrysingle-step analysischildren’s serum |
| spellingShingle | Luterotti Svjetlana Kordić Tončica Vukman Dodig Slavica Contribution to diagnostics/prognostics of tuberculosis in children. I. New methods of assaying zinc and simultaneously copper and zinc in diluted sera by flame atomic-absorption spectrometry Acta Pharmaceutica copper zinc flame atomic-absorption spectrometry single-step analysis children’s serum |
| title | Contribution to diagnostics/prognostics of tuberculosis in children. I. New methods of assaying zinc and simultaneously copper and zinc in diluted sera by flame atomic-absorption spectrometry |
| title_full | Contribution to diagnostics/prognostics of tuberculosis in children. I. New methods of assaying zinc and simultaneously copper and zinc in diluted sera by flame atomic-absorption spectrometry |
| title_fullStr | Contribution to diagnostics/prognostics of tuberculosis in children. I. New methods of assaying zinc and simultaneously copper and zinc in diluted sera by flame atomic-absorption spectrometry |
| title_full_unstemmed | Contribution to diagnostics/prognostics of tuberculosis in children. I. New methods of assaying zinc and simultaneously copper and zinc in diluted sera by flame atomic-absorption spectrometry |
| title_short | Contribution to diagnostics/prognostics of tuberculosis in children. I. New methods of assaying zinc and simultaneously copper and zinc in diluted sera by flame atomic-absorption spectrometry |
| title_sort | contribution to diagnostics prognostics of tuberculosis in children i new methods of assaying zinc and simultaneously copper and zinc in diluted sera by flame atomic absorption spectrometry |
| topic | copper zinc flame atomic-absorption spectrometry single-step analysis children’s serum |
| url | https://doi.org/10.1515/acph-2015-0026 |
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