Protective effects of Atractylodes macrocephala polysaccharides on acetaminophen-induced liver injury
BackgroundDrug-induced liver injury (DILI) is a major clinical concern due to its unpredictable nature and lack of effective therapeutic options.MethodsThis study investigated the hepatoprotective effects of Atractylodes macrocephala polysaccharides (AMPs) in a mouse model of acetaminophen (APAP)-in...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2025.1583334/full |
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| author | Jiali Wu Biao Jia Shuai Gong Yangpeng Li Jiaqi Wang Yuqiao Huang Jiao Guo |
| author_facet | Jiali Wu Biao Jia Shuai Gong Yangpeng Li Jiaqi Wang Yuqiao Huang Jiao Guo |
| author_sort | Jiali Wu |
| collection | DOAJ |
| description | BackgroundDrug-induced liver injury (DILI) is a major clinical concern due to its unpredictable nature and lack of effective therapeutic options.MethodsThis study investigated the hepatoprotective effects of Atractylodes macrocephala polysaccharides (AMPs) in a mouse model of acetaminophen (APAP)-induced liver injury. Mice were pretreated with AMPs for 7 days prior to APAP challenge, and liver injury was evaluated through histopathology, serum biochemistry, molecular assays, and gut microbiota analysis.ResultsAMPs treatment significantly reduced serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels compared to the APAP group (p < 0.05). Hepatic oxidative stress was alleviated, as indicated by increased levels of glutathione (GSH, p < 0.05) and superoxide dismutase (SOD, p < 0.05), and reduced malondialdehyde (MDA, p < 0.05). AMPs also suppressed inflammatory cytokines, including Il-1β, Tnf-α, Il-6, and Nlrp3 (p < 0.05), and modulated apoptosis-related proteins by downregulating Bax and upregulating Bcl-2 and Bcl-xl expression (p < 0.05). Furthermore, AMPs improved gut microbiota diversity and enriched beneficial genera such as Roseburia, as revealed by 16S rDNA sequencing. Fecal microbiota transplantation from AMPs-treated mice replicated these hepatoprotective effects, highlighting the involvement of the gut–liver axis.ConclusionThese findings support the therapeutic potential of AMPs as a multifaceted agent for DILI, exerting protective effects through modulation of oxidative stress, inflammation, apoptosis, and intestinal dysbiosis. |
| format | Article |
| id | doaj-art-68cd67e94de041beba41a0bb16cefd1d |
| institution | OA Journals |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj-art-68cd67e94de041beba41a0bb16cefd1d2025-08-20T02:10:09ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-06-011610.3389/fphar.2025.15833341583334Protective effects of Atractylodes macrocephala polysaccharides on acetaminophen-induced liver injuryJiali WuBiao JiaShuai GongYangpeng LiJiaqi WangYuqiao HuangJiao GuoBackgroundDrug-induced liver injury (DILI) is a major clinical concern due to its unpredictable nature and lack of effective therapeutic options.MethodsThis study investigated the hepatoprotective effects of Atractylodes macrocephala polysaccharides (AMPs) in a mouse model of acetaminophen (APAP)-induced liver injury. Mice were pretreated with AMPs for 7 days prior to APAP challenge, and liver injury was evaluated through histopathology, serum biochemistry, molecular assays, and gut microbiota analysis.ResultsAMPs treatment significantly reduced serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels compared to the APAP group (p < 0.05). Hepatic oxidative stress was alleviated, as indicated by increased levels of glutathione (GSH, p < 0.05) and superoxide dismutase (SOD, p < 0.05), and reduced malondialdehyde (MDA, p < 0.05). AMPs also suppressed inflammatory cytokines, including Il-1β, Tnf-α, Il-6, and Nlrp3 (p < 0.05), and modulated apoptosis-related proteins by downregulating Bax and upregulating Bcl-2 and Bcl-xl expression (p < 0.05). Furthermore, AMPs improved gut microbiota diversity and enriched beneficial genera such as Roseburia, as revealed by 16S rDNA sequencing. Fecal microbiota transplantation from AMPs-treated mice replicated these hepatoprotective effects, highlighting the involvement of the gut–liver axis.ConclusionThese findings support the therapeutic potential of AMPs as a multifaceted agent for DILI, exerting protective effects through modulation of oxidative stress, inflammation, apoptosis, and intestinal dysbiosis.https://www.frontiersin.org/articles/10.3389/fphar.2025.1583334/fullhepatoprotectionherbal polysaccharidesintestinal microbiotaoxidative stressNLRP3 inflammasome |
| spellingShingle | Jiali Wu Biao Jia Shuai Gong Yangpeng Li Jiaqi Wang Yuqiao Huang Jiao Guo Protective effects of Atractylodes macrocephala polysaccharides on acetaminophen-induced liver injury Frontiers in Pharmacology hepatoprotection herbal polysaccharides intestinal microbiota oxidative stress NLRP3 inflammasome |
| title | Protective effects of Atractylodes macrocephala polysaccharides on acetaminophen-induced liver injury |
| title_full | Protective effects of Atractylodes macrocephala polysaccharides on acetaminophen-induced liver injury |
| title_fullStr | Protective effects of Atractylodes macrocephala polysaccharides on acetaminophen-induced liver injury |
| title_full_unstemmed | Protective effects of Atractylodes macrocephala polysaccharides on acetaminophen-induced liver injury |
| title_short | Protective effects of Atractylodes macrocephala polysaccharides on acetaminophen-induced liver injury |
| title_sort | protective effects of atractylodes macrocephala polysaccharides on acetaminophen induced liver injury |
| topic | hepatoprotection herbal polysaccharides intestinal microbiota oxidative stress NLRP3 inflammasome |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2025.1583334/full |
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