Ultrabright contrast agents with synergistic Raman enhancements for precise intraoperative imaging and photothermal ablation of orthotopic tumor models
Abstract Background Intraoperative imaging is critical for achieving precise cancer resection. Among available techniques, Raman spectral imaging emerges as a promising modality due to its high spatial resolution and signal stability. However, its clinical application for in vivo imaging is limited...
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| Format: | Article |
| Language: | English |
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BMC
2025-01-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-025-03099-2 |
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| author | Yiqun Ma Shuchi Xia Annan Hu Qianyi Zhang Zhengzhong Shao Bo Tian Qinrui Lin |
| author_facet | Yiqun Ma Shuchi Xia Annan Hu Qianyi Zhang Zhengzhong Shao Bo Tian Qinrui Lin |
| author_sort | Yiqun Ma |
| collection | DOAJ |
| description | Abstract Background Intraoperative imaging is critical for achieving precise cancer resection. Among available techniques, Raman spectral imaging emerges as a promising modality due to its high spatial resolution and signal stability. However, its clinical application for in vivo imaging is limited by the inherently weak Raman scattering signal. To address this challenge, we developed a novel strategy that integrates two enhancement mechanisms into a single Raman contrast agent. Results This contrast agent exploits the synergistic effects of an anisotropic gold nanorod and a polypyrrole-polydopamine hybrid, resulting in a substantial amplification of Raman signals. Consequently, the agent enables clear delineation of malignant tissues in both orthotopic and subcutaneous tumor models. Beyond its imaging capability, the agent also facilitates photothermal ablation, providing a long-term solution for suppressing tumor recurrence. Conclusion This study systematically evaluates the imaging performance of the synthesized Raman contrast agents across different tumor models and highlights the critical role of optimizing the aspect ratio of anisotropic agents for in vivo imaging. By offering a dual-function Raman contrast agent, this research advances the potential of Raman spectral imaging for intraoperative applications and clinical translation. Graphical abstract |
| format | Article |
| id | doaj-art-68c7ee7e99124ad2a88d1a2a92cdf35c |
| institution | Kabale University |
| issn | 1477-3155 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-68c7ee7e99124ad2a88d1a2a92cdf35c2025-01-19T12:37:51ZengBMCJournal of Nanobiotechnology1477-31552025-01-0123111610.1186/s12951-025-03099-2Ultrabright contrast agents with synergistic Raman enhancements for precise intraoperative imaging and photothermal ablation of orthotopic tumor modelsYiqun Ma0Shuchi Xia1Annan Hu2Qianyi Zhang3Zhengzhong Shao4Bo Tian5Qinrui Lin6Department of Orthopaedic Surgery, Zhongshan Hospital, Fudan UniversityDepartment of Dentistry, Zhongshan Hospital, Fudan UniversityDepartment of Orthopaedic Surgery, Zhongshan Hospital, Fudan UniversityDepartment of Orthopaedic Surgery, Zhongshan Hospital, Fudan UniversityState Key Laboratory of Molecular Engineering of Polymers, Fudan UniversityDepartment of Orthopaedic Surgery, Zhongshan Hospital, Fudan UniversityState Key Laboratory of Molecular Engineering of Polymers, Fudan UniversityAbstract Background Intraoperative imaging is critical for achieving precise cancer resection. Among available techniques, Raman spectral imaging emerges as a promising modality due to its high spatial resolution and signal stability. However, its clinical application for in vivo imaging is limited by the inherently weak Raman scattering signal. To address this challenge, we developed a novel strategy that integrates two enhancement mechanisms into a single Raman contrast agent. Results This contrast agent exploits the synergistic effects of an anisotropic gold nanorod and a polypyrrole-polydopamine hybrid, resulting in a substantial amplification of Raman signals. Consequently, the agent enables clear delineation of malignant tissues in both orthotopic and subcutaneous tumor models. Beyond its imaging capability, the agent also facilitates photothermal ablation, providing a long-term solution for suppressing tumor recurrence. Conclusion This study systematically evaluates the imaging performance of the synthesized Raman contrast agents across different tumor models and highlights the critical role of optimizing the aspect ratio of anisotropic agents for in vivo imaging. By offering a dual-function Raman contrast agent, this research advances the potential of Raman spectral imaging for intraoperative applications and clinical translation. Graphical abstracthttps://doi.org/10.1186/s12951-025-03099-2Intra-operative imagingSurface-enhanced Raman spectroscopy (SERS)Resonance Raman spectroscopy (RRS)Conjugated polymersGold nanorods |
| spellingShingle | Yiqun Ma Shuchi Xia Annan Hu Qianyi Zhang Zhengzhong Shao Bo Tian Qinrui Lin Ultrabright contrast agents with synergistic Raman enhancements for precise intraoperative imaging and photothermal ablation of orthotopic tumor models Journal of Nanobiotechnology Intra-operative imaging Surface-enhanced Raman spectroscopy (SERS) Resonance Raman spectroscopy (RRS) Conjugated polymers Gold nanorods |
| title | Ultrabright contrast agents with synergistic Raman enhancements for precise intraoperative imaging and photothermal ablation of orthotopic tumor models |
| title_full | Ultrabright contrast agents with synergistic Raman enhancements for precise intraoperative imaging and photothermal ablation of orthotopic tumor models |
| title_fullStr | Ultrabright contrast agents with synergistic Raman enhancements for precise intraoperative imaging and photothermal ablation of orthotopic tumor models |
| title_full_unstemmed | Ultrabright contrast agents with synergistic Raman enhancements for precise intraoperative imaging and photothermal ablation of orthotopic tumor models |
| title_short | Ultrabright contrast agents with synergistic Raman enhancements for precise intraoperative imaging and photothermal ablation of orthotopic tumor models |
| title_sort | ultrabright contrast agents with synergistic raman enhancements for precise intraoperative imaging and photothermal ablation of orthotopic tumor models |
| topic | Intra-operative imaging Surface-enhanced Raman spectroscopy (SERS) Resonance Raman spectroscopy (RRS) Conjugated polymers Gold nanorods |
| url | https://doi.org/10.1186/s12951-025-03099-2 |
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