The involvement of CgCaspase-3 in regulating the expressions of proinflammatory cytokines through NF-κB/Rel signaling pathway in oysters
Cysteinyl aspartate specific proteinase-3 (Caspase-3) is a prominent mediator of apoptosis and participates in the cell death signaling cascade. In our previous study, CgCaspase-3 identified from Crassostrea gigas was involved in CgGSDME-dependent pyroptosis. In the present study, CgCaspase-3 was de...
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Elsevier
2025-06-01
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| Series: | Comparative Immunology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950311625000096 |
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| author | Zihan Wang Xiaopeng Li Jiejie Sun Shuyi Mu Wei Wu Renle Chang Lingling Wang Linsheng Song |
| author_facet | Zihan Wang Xiaopeng Li Jiejie Sun Shuyi Mu Wei Wu Renle Chang Lingling Wang Linsheng Song |
| author_sort | Zihan Wang |
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| description | Cysteinyl aspartate specific proteinase-3 (Caspase-3) is a prominent mediator of apoptosis and participates in the cell death signaling cascade. In our previous study, CgCaspase-3 identified from Crassostrea gigas was involved in CgGSDME-dependent pyroptosis. In the present study, CgCaspase-3 was demonstrated to regulate the expressions of proinflammatory cytokines through nuclear factor kappa B (NF-κB) signaling pathway. In haemocytes of Ac-DEVD-CHO-treated and CgCaspase-3-RNAi oysters, the nuclear translocation of NF-κB/Rel was inhibited at 24 h after Vibrio splendidus stimulation. The mRNA expressions of interleukins 17s (CgIL17–2, CgIL17–3, and CgIL17–6), tumor necrosis factor-1 (CgTNF-1), high mobility group box-1 protein (CgHMGB1) and interferon-like protein (CgIFNLP) were significantly decreased in haemocytes of CgCaspase-3-RNAi or CgRel-RNAi oysters, respectively. In addition, the phenomenon of gill swelling and cilia shedding was reduced in CgCaspase-3-RNAi oysters after V. splendidus stimulation. These results indicated that CgCaspase-3 was involved in regulating bacteria-induced inflammation through NF-κB/Rel signaling pathway. |
| format | Article |
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| institution | Kabale University |
| issn | 2950-3116 |
| language | English |
| publishDate | 2025-06-01 |
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| series | Comparative Immunology Reports |
| spelling | doaj-art-68ae2a2882ff4bbeb1fa673ff339a9e82025-01-31T05:12:53ZengElsevierComparative Immunology Reports2950-31162025-06-018200203The involvement of CgCaspase-3 in regulating the expressions of proinflammatory cytokines through NF-κB/Rel signaling pathway in oystersZihan Wang0Xiaopeng Li1Jiejie Sun2Shuyi Mu3Wei Wu4Renle Chang5Lingling Wang6Linsheng Song7Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China; Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, ChinaLiaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China; Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, ChinaLiaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China; Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China; Corresponding authors at: Dalian Ocean University 52 Heishijiao Street, Dalian 116023, China.Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China; Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, ChinaLiaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China; Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, ChinaLiaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China; Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, ChinaLiaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China; Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian 116023, ChinaLiaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China; Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China; Corresponding authors at: Dalian Ocean University 52 Heishijiao Street, Dalian 116023, China.Cysteinyl aspartate specific proteinase-3 (Caspase-3) is a prominent mediator of apoptosis and participates in the cell death signaling cascade. In our previous study, CgCaspase-3 identified from Crassostrea gigas was involved in CgGSDME-dependent pyroptosis. In the present study, CgCaspase-3 was demonstrated to regulate the expressions of proinflammatory cytokines through nuclear factor kappa B (NF-κB) signaling pathway. In haemocytes of Ac-DEVD-CHO-treated and CgCaspase-3-RNAi oysters, the nuclear translocation of NF-κB/Rel was inhibited at 24 h after Vibrio splendidus stimulation. The mRNA expressions of interleukins 17s (CgIL17–2, CgIL17–3, and CgIL17–6), tumor necrosis factor-1 (CgTNF-1), high mobility group box-1 protein (CgHMGB1) and interferon-like protein (CgIFNLP) were significantly decreased in haemocytes of CgCaspase-3-RNAi or CgRel-RNAi oysters, respectively. In addition, the phenomenon of gill swelling and cilia shedding was reduced in CgCaspase-3-RNAi oysters after V. splendidus stimulation. These results indicated that CgCaspase-3 was involved in regulating bacteria-induced inflammation through NF-κB/Rel signaling pathway.http://www.sciencedirect.com/science/article/pii/S2950311625000096InflammationProinflammatory cytokineCaspase-3NF-κBCrassostrea gigas |
| spellingShingle | Zihan Wang Xiaopeng Li Jiejie Sun Shuyi Mu Wei Wu Renle Chang Lingling Wang Linsheng Song The involvement of CgCaspase-3 in regulating the expressions of proinflammatory cytokines through NF-κB/Rel signaling pathway in oysters Comparative Immunology Reports Inflammation Proinflammatory cytokine Caspase-3 NF-κB Crassostrea gigas |
| title | The involvement of CgCaspase-3 in regulating the expressions of proinflammatory cytokines through NF-κB/Rel signaling pathway in oysters |
| title_full | The involvement of CgCaspase-3 in regulating the expressions of proinflammatory cytokines through NF-κB/Rel signaling pathway in oysters |
| title_fullStr | The involvement of CgCaspase-3 in regulating the expressions of proinflammatory cytokines through NF-κB/Rel signaling pathway in oysters |
| title_full_unstemmed | The involvement of CgCaspase-3 in regulating the expressions of proinflammatory cytokines through NF-κB/Rel signaling pathway in oysters |
| title_short | The involvement of CgCaspase-3 in regulating the expressions of proinflammatory cytokines through NF-κB/Rel signaling pathway in oysters |
| title_sort | involvement of cgcaspase 3 in regulating the expressions of proinflammatory cytokines through nf κb rel signaling pathway in oysters |
| topic | Inflammation Proinflammatory cytokine Caspase-3 NF-κB Crassostrea gigas |
| url | http://www.sciencedirect.com/science/article/pii/S2950311625000096 |
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