Antibodies Recognizing Yersinia enterocolitica Lipopolysaccharides of Various Chemotypes in Synovial Fluids From Patients With Juvenile Idiopathic Arthritis
Yersinia enterocolitica O:3 (YeO3) is considered to be associated with reactive arthritis (ReA), and its lipopolysaccharide (LPS) has been detected in synovial fluids from patients. Interestingly, YeO3 wild-type LPS was processed by host cells, resulting in truncated LPS molecules presenting the cor...
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| Format: | Article |
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Wiley
2022-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/9627934 |
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| author | Katarzyna Kasperkiewicz Anna S. Świerzko Mateusz Michalski Łukasz Eppa Mikael Skurnik Zbigniew Żuber Maciej Cedzyński |
| author_facet | Katarzyna Kasperkiewicz Anna S. Świerzko Mateusz Michalski Łukasz Eppa Mikael Skurnik Zbigniew Żuber Maciej Cedzyński |
| author_sort | Katarzyna Kasperkiewicz |
| collection | DOAJ |
| description | Yersinia enterocolitica O:3 (YeO3) is considered to be associated with reactive arthritis (ReA), and its lipopolysaccharide (LPS) has been detected in synovial fluids from patients. Interestingly, YeO3 wild-type LPS was processed by host cells, resulting in truncated LPS molecules presenting the core region. Previously, we reported the immunogenicity but not adjuvanticity of YeO3 LPSs of wild (S) type, Ra, Rd, or Re chemotypes in mice. Here, we demonstrate the presence of YeO3 LPS chemotype-specific antibodies in all analyzed synovial fluids (SF) from patients with juvenile idiopathic arthritis (JIA). Interestingly, the high titer of antibodies specific for the Kdo-lipid A region was found in most tested SF. In contrast, only a few were positive for antibodies recognizing O-specific polysaccharides. Western blot analysis revealed the presence of antibodies reacting with fast-migrating LPS fractions and enterobacterial common antigen (ECA) in synovial fluid samples. Our data also suggest the importance of LPS-associated ECA for the antigenicity of endotoxin. Furthermore, we confirmed in vitro that Yersinia LPS processing leads to the exposure of its core region and enhanced potency of complement lectin pathway activation. |
| format | Article |
| id | doaj-art-689414edc96e426eb8d5c6e6f1577faa |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-689414edc96e426eb8d5c6e6f1577faa2025-02-03T06:00:55ZengWileyJournal of Immunology Research2314-71562022-01-01202210.1155/2022/9627934Antibodies Recognizing Yersinia enterocolitica Lipopolysaccharides of Various Chemotypes in Synovial Fluids From Patients With Juvenile Idiopathic ArthritisKatarzyna Kasperkiewicz0Anna S. Świerzko1Mateusz Michalski2Łukasz Eppa3Mikael Skurnik4Zbigniew Żuber5Maciej Cedzyński6Institute of BiologyLaboratory of Immunobiology of InfectionsLaboratory of Immunobiology of InfectionsLaboratory of Immunobiology of InfectionsDepartment of Bacteriology and ImmunologyPediatrics ClinicLaboratory of Immunobiology of InfectionsYersinia enterocolitica O:3 (YeO3) is considered to be associated with reactive arthritis (ReA), and its lipopolysaccharide (LPS) has been detected in synovial fluids from patients. Interestingly, YeO3 wild-type LPS was processed by host cells, resulting in truncated LPS molecules presenting the core region. Previously, we reported the immunogenicity but not adjuvanticity of YeO3 LPSs of wild (S) type, Ra, Rd, or Re chemotypes in mice. Here, we demonstrate the presence of YeO3 LPS chemotype-specific antibodies in all analyzed synovial fluids (SF) from patients with juvenile idiopathic arthritis (JIA). Interestingly, the high titer of antibodies specific for the Kdo-lipid A region was found in most tested SF. In contrast, only a few were positive for antibodies recognizing O-specific polysaccharides. Western blot analysis revealed the presence of antibodies reacting with fast-migrating LPS fractions and enterobacterial common antigen (ECA) in synovial fluid samples. Our data also suggest the importance of LPS-associated ECA for the antigenicity of endotoxin. Furthermore, we confirmed in vitro that Yersinia LPS processing leads to the exposure of its core region and enhanced potency of complement lectin pathway activation.http://dx.doi.org/10.1155/2022/9627934 |
| spellingShingle | Katarzyna Kasperkiewicz Anna S. Świerzko Mateusz Michalski Łukasz Eppa Mikael Skurnik Zbigniew Żuber Maciej Cedzyński Antibodies Recognizing Yersinia enterocolitica Lipopolysaccharides of Various Chemotypes in Synovial Fluids From Patients With Juvenile Idiopathic Arthritis Journal of Immunology Research |
| title | Antibodies Recognizing Yersinia enterocolitica Lipopolysaccharides of Various Chemotypes in Synovial Fluids From Patients With Juvenile Idiopathic Arthritis |
| title_full | Antibodies Recognizing Yersinia enterocolitica Lipopolysaccharides of Various Chemotypes in Synovial Fluids From Patients With Juvenile Idiopathic Arthritis |
| title_fullStr | Antibodies Recognizing Yersinia enterocolitica Lipopolysaccharides of Various Chemotypes in Synovial Fluids From Patients With Juvenile Idiopathic Arthritis |
| title_full_unstemmed | Antibodies Recognizing Yersinia enterocolitica Lipopolysaccharides of Various Chemotypes in Synovial Fluids From Patients With Juvenile Idiopathic Arthritis |
| title_short | Antibodies Recognizing Yersinia enterocolitica Lipopolysaccharides of Various Chemotypes in Synovial Fluids From Patients With Juvenile Idiopathic Arthritis |
| title_sort | antibodies recognizing yersinia enterocolitica lipopolysaccharides of various chemotypes in synovial fluids from patients with juvenile idiopathic arthritis |
| url | http://dx.doi.org/10.1155/2022/9627934 |
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