Evaluation of Genotoxic and Cytotoxic Effects in Human Peripheral Blood Lymphocytes Exposed In Vitro to Neonicotinoid Insecticides News
Calypso (thiacloprid), Poncho (clothianidin), Gaucho (imidacloprid), and Jade (imidacloprid) are commercial neonicotinoid insecticides, a new class of agrochemicals in México. However, genotoxic and cytotoxic studies have not been performed. In the present study, human peripheral blood lymphocytes (...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2012/612647 |
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| author | María Elena Calderón-Segura Sandra Gómez-Arroyo Rafael Villalobos-Pietrini Carmen Martínez-Valenzuela Yolanda Carbajal-López María del Carmen Calderón-Ezquerro Josefina Cortés-Eslava Rocío García-Martínez Diana Flores-Ramírez María Isabel Rodríguez-Romero Patricia Méndez-Pérez Enrique Bañuelos-Ruíz |
| author_facet | María Elena Calderón-Segura Sandra Gómez-Arroyo Rafael Villalobos-Pietrini Carmen Martínez-Valenzuela Yolanda Carbajal-López María del Carmen Calderón-Ezquerro Josefina Cortés-Eslava Rocío García-Martínez Diana Flores-Ramírez María Isabel Rodríguez-Romero Patricia Méndez-Pérez Enrique Bañuelos-Ruíz |
| author_sort | María Elena Calderón-Segura |
| collection | DOAJ |
| description | Calypso (thiacloprid), Poncho (clothianidin), Gaucho (imidacloprid), and Jade (imidacloprid) are commercial neonicotinoid insecticides, a new class of agrochemicals in México. However, genotoxic and cytotoxic studies have not been performed. In the present study, human peripheral blood lymphocytes (PBL) were exposed in vitro to different concentrations of the four insecticides. The genotoxic and cytotoxic effects were evaluated using the alkaline comet and trypan blue dye exclusion assays. DNA damage was evaluated using two genotoxicity parameters: tail length and comet frequency. Exposure to 9.5×10-6 to 5.7×10-5 M Jade; 2.8×10-4 to 1.7×10-3 M Gaucho; 0.6×10-1 to 1.4×10-1 M Calypso; 1.2×10-1 to 9.5×10-1 M Poncho for 2 h induced a significant increase DNA damage with a concentration-dependent relationship. Jade was the most genotoxic of the four insecticides studied. Cytotoxicity was observed in cells exposed to 18×10-3 M Jade, 2.0×10-3 M Gaucho, 2.0×10-1 M Calypso, 1.07 M Poncho, and cell death occurred at 30×10-3 M Jade, 3.3×10-3 M Gaucho, 2.8×10-1 M Calypso, and 1.42 M Poncho. This study provides the first report of genotoxic and cytotoxic effects in PBL following in vitro exposure to commercial neonicotinoid insecticides. |
| format | Article |
| id | doaj-art-6880b6039d7c4e17b393835208a2259f |
| institution | Kabale University |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-6880b6039d7c4e17b393835208a2259f2025-02-03T01:02:36ZengWileyJournal of Toxicology1687-81911687-82052012-01-01201210.1155/2012/612647612647Evaluation of Genotoxic and Cytotoxic Effects in Human Peripheral Blood Lymphocytes Exposed In Vitro to Neonicotinoid Insecticides NewsMaría Elena Calderón-Segura0Sandra Gómez-Arroyo1Rafael Villalobos-Pietrini2Carmen Martínez-Valenzuela3Yolanda Carbajal-López4María del Carmen Calderón-Ezquerro5Josefina Cortés-Eslava6Rocío García-Martínez7Diana Flores-Ramírez8María Isabel Rodríguez-Romero9Patricia Méndez-Pérez10Enrique Bañuelos-Ruíz11Laboratorios de Citogenética y Mutagénesis Ambientales, Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México, Ciudad Universitaria Coyoacán, 04510 México city, DF, MexicoLaboratorios de Citogenética y Mutagénesis Ambientales, Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México, Ciudad Universitaria Coyoacán, 04510 México city, DF, MexicoLaboratorios de Citogenética y Mutagénesis Ambientales, Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México, Ciudad Universitaria Coyoacán, 04510 México city, DF, MexicoDepartamento de Ciencias Biológicas, Universidad de Occidente, Boulevard Macario Gaxiola, Carretera Internacional, Los Mochis, SIN, 81223 México, MexicoUnidad Académica de Ciencias Químico Biológicas, Avenida Lázaro Cárdenas S/N, Universidad Autónoma de Guerrero, Ciudad Universitaria, Chilpancingo, GRO, 39090 México, MexicoLaboratorios de Citogenética y Mutagénesis Ambientales, Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México, Ciudad Universitaria Coyoacán, 04510 México city, DF, MexicoLaboratorios de Citogenética y Mutagénesis Ambientales, Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México, Ciudad Universitaria Coyoacán, 04510 México city, DF, MexicoLaboratorio de Química Atmosférica, Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México, Ciudad Universitaria Coyoacán, 04510 México, MexicoLaboratorios de Citogenética y Mutagénesis Ambientales, Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México, Ciudad Universitaria Coyoacán, 04510 México city, DF, MexicoLaboratorios de Citogenética y Mutagénesis Ambientales, Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México, Ciudad Universitaria Coyoacán, 04510 México city, DF, MexicoLaboratorios de Citogenética y Mutagénesis Ambientales, Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México, Ciudad Universitaria Coyoacán, 04510 México city, DF, MexicoLaboratorios de Citogenética y Mutagénesis Ambientales, Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México, Ciudad Universitaria Coyoacán, 04510 México city, DF, MexicoCalypso (thiacloprid), Poncho (clothianidin), Gaucho (imidacloprid), and Jade (imidacloprid) are commercial neonicotinoid insecticides, a new class of agrochemicals in México. However, genotoxic and cytotoxic studies have not been performed. In the present study, human peripheral blood lymphocytes (PBL) were exposed in vitro to different concentrations of the four insecticides. The genotoxic and cytotoxic effects were evaluated using the alkaline comet and trypan blue dye exclusion assays. DNA damage was evaluated using two genotoxicity parameters: tail length and comet frequency. Exposure to 9.5×10-6 to 5.7×10-5 M Jade; 2.8×10-4 to 1.7×10-3 M Gaucho; 0.6×10-1 to 1.4×10-1 M Calypso; 1.2×10-1 to 9.5×10-1 M Poncho for 2 h induced a significant increase DNA damage with a concentration-dependent relationship. Jade was the most genotoxic of the four insecticides studied. Cytotoxicity was observed in cells exposed to 18×10-3 M Jade, 2.0×10-3 M Gaucho, 2.0×10-1 M Calypso, 1.07 M Poncho, and cell death occurred at 30×10-3 M Jade, 3.3×10-3 M Gaucho, 2.8×10-1 M Calypso, and 1.42 M Poncho. This study provides the first report of genotoxic and cytotoxic effects in PBL following in vitro exposure to commercial neonicotinoid insecticides.http://dx.doi.org/10.1155/2012/612647 |
| spellingShingle | María Elena Calderón-Segura Sandra Gómez-Arroyo Rafael Villalobos-Pietrini Carmen Martínez-Valenzuela Yolanda Carbajal-López María del Carmen Calderón-Ezquerro Josefina Cortés-Eslava Rocío García-Martínez Diana Flores-Ramírez María Isabel Rodríguez-Romero Patricia Méndez-Pérez Enrique Bañuelos-Ruíz Evaluation of Genotoxic and Cytotoxic Effects in Human Peripheral Blood Lymphocytes Exposed In Vitro to Neonicotinoid Insecticides News Journal of Toxicology |
| title | Evaluation of Genotoxic and Cytotoxic Effects in Human Peripheral Blood Lymphocytes Exposed In Vitro to Neonicotinoid Insecticides News |
| title_full | Evaluation of Genotoxic and Cytotoxic Effects in Human Peripheral Blood Lymphocytes Exposed In Vitro to Neonicotinoid Insecticides News |
| title_fullStr | Evaluation of Genotoxic and Cytotoxic Effects in Human Peripheral Blood Lymphocytes Exposed In Vitro to Neonicotinoid Insecticides News |
| title_full_unstemmed | Evaluation of Genotoxic and Cytotoxic Effects in Human Peripheral Blood Lymphocytes Exposed In Vitro to Neonicotinoid Insecticides News |
| title_short | Evaluation of Genotoxic and Cytotoxic Effects in Human Peripheral Blood Lymphocytes Exposed In Vitro to Neonicotinoid Insecticides News |
| title_sort | evaluation of genotoxic and cytotoxic effects in human peripheral blood lymphocytes exposed in vitro to neonicotinoid insecticides news |
| url | http://dx.doi.org/10.1155/2012/612647 |
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