The Three-Dimensional Culture System with Matrigel and Neurotrophic Factors Preserves the Structure and Function of Spiral Ganglion Neuron In Vitro
Whole organ culture of the spiral ganglion region is a resourceful model system facilitating manipulation and analysis of live sprial ganglion neurons (SGNs). Three-dimensional (3D) cultures have been demonstrated to have many biomedical applications, but the effect of 3D culture in maintaining the...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2016/4280407 |
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| author | Gaoying Sun Wenwen Liu Zhaomin Fan Daogong Zhang Yuechen Han Lei Xu Jieyu Qi Shasha Zhang Bradley T. Gao Xiaohui Bai Jianfeng Li Renjie Chai Haibo Wang |
| author_facet | Gaoying Sun Wenwen Liu Zhaomin Fan Daogong Zhang Yuechen Han Lei Xu Jieyu Qi Shasha Zhang Bradley T. Gao Xiaohui Bai Jianfeng Li Renjie Chai Haibo Wang |
| author_sort | Gaoying Sun |
| collection | DOAJ |
| description | Whole organ culture of the spiral ganglion region is a resourceful model system facilitating manipulation and analysis of live sprial ganglion neurons (SGNs). Three-dimensional (3D) cultures have been demonstrated to have many biomedical applications, but the effect of 3D culture in maintaining the SGNs structure and function in explant culture remains uninvestigated. In this study, we used the matrigel to encapsulate the spiral ganglion region isolated from neonatal mice. First, we optimized the matrigel concentration for the 3D culture system and found the 3D culture system protected the SGNs against apoptosis, preserved the structure of spiral ganglion region, and promoted the sprouting and outgrowth of SGNs neurites. Next, we found the 3D culture system promoted growth cone growth as evidenced by a higher average number and a longer average length of filopodia and a larger growth cone area. 3D culture system also significantly elevated the synapse density of SGNs. Last, we found that the 3D culture system combined with neurotrophic factors had accumulated effects in promoting the neurites outgrowth compared with 3D culture or NFs treatment only groups. Together, we conclude that the 3D culture system preserves the structure and function of SGN in explant culture. |
| format | Article |
| id | doaj-art-687b6ce95584437db871d01e956f387e |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-687b6ce95584437db871d01e956f387e2025-02-03T06:14:18ZengWileyNeural Plasticity2090-59041687-54432016-01-01201610.1155/2016/42804074280407The Three-Dimensional Culture System with Matrigel and Neurotrophic Factors Preserves the Structure and Function of Spiral Ganglion Neuron In VitroGaoying Sun0Wenwen Liu1Zhaomin Fan2Daogong Zhang3Yuechen Han4Lei Xu5Jieyu Qi6Shasha Zhang7Bradley T. Gao8Xiaohui Bai9Jianfeng Li10Renjie Chai11Haibo Wang12Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250022, ChinaOtolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250022, ChinaOtolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250022, ChinaOtolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250022, ChinaOtolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250022, ChinaOtolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250022, ChinaKey Laboratory for Developmental Genes and Human Disease, Ministry of Education, Institute of Life Sciences, Southeast University, Nanjing 210096, ChinaKey Laboratory for Developmental Genes and Human Disease, Ministry of Education, Institute of Life Sciences, Southeast University, Nanjing 210096, ChinaCollege of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USAOtolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250022, ChinaOtolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250022, ChinaKey Laboratory for Developmental Genes and Human Disease, Ministry of Education, Institute of Life Sciences, Southeast University, Nanjing 210096, ChinaOtolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250022, ChinaWhole organ culture of the spiral ganglion region is a resourceful model system facilitating manipulation and analysis of live sprial ganglion neurons (SGNs). Three-dimensional (3D) cultures have been demonstrated to have many biomedical applications, but the effect of 3D culture in maintaining the SGNs structure and function in explant culture remains uninvestigated. In this study, we used the matrigel to encapsulate the spiral ganglion region isolated from neonatal mice. First, we optimized the matrigel concentration for the 3D culture system and found the 3D culture system protected the SGNs against apoptosis, preserved the structure of spiral ganglion region, and promoted the sprouting and outgrowth of SGNs neurites. Next, we found the 3D culture system promoted growth cone growth as evidenced by a higher average number and a longer average length of filopodia and a larger growth cone area. 3D culture system also significantly elevated the synapse density of SGNs. Last, we found that the 3D culture system combined with neurotrophic factors had accumulated effects in promoting the neurites outgrowth compared with 3D culture or NFs treatment only groups. Together, we conclude that the 3D culture system preserves the structure and function of SGN in explant culture.http://dx.doi.org/10.1155/2016/4280407 |
| spellingShingle | Gaoying Sun Wenwen Liu Zhaomin Fan Daogong Zhang Yuechen Han Lei Xu Jieyu Qi Shasha Zhang Bradley T. Gao Xiaohui Bai Jianfeng Li Renjie Chai Haibo Wang The Three-Dimensional Culture System with Matrigel and Neurotrophic Factors Preserves the Structure and Function of Spiral Ganglion Neuron In Vitro Neural Plasticity |
| title | The Three-Dimensional Culture System with Matrigel and Neurotrophic Factors Preserves the Structure and Function of Spiral Ganglion Neuron In Vitro |
| title_full | The Three-Dimensional Culture System with Matrigel and Neurotrophic Factors Preserves the Structure and Function of Spiral Ganglion Neuron In Vitro |
| title_fullStr | The Three-Dimensional Culture System with Matrigel and Neurotrophic Factors Preserves the Structure and Function of Spiral Ganglion Neuron In Vitro |
| title_full_unstemmed | The Three-Dimensional Culture System with Matrigel and Neurotrophic Factors Preserves the Structure and Function of Spiral Ganglion Neuron In Vitro |
| title_short | The Three-Dimensional Culture System with Matrigel and Neurotrophic Factors Preserves the Structure and Function of Spiral Ganglion Neuron In Vitro |
| title_sort | three dimensional culture system with matrigel and neurotrophic factors preserves the structure and function of spiral ganglion neuron in vitro |
| url | http://dx.doi.org/10.1155/2016/4280407 |
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