Effects of Dietary Nano-Composite of Copper and Carbon on Antioxidant Capacity, Immunity, and Cecal Microbiota of Weaned Ira White Rabbits
This experiment investigated the effects of dietary supplementation with nano-composites of copper and carbon (NCCC) on antioxidants, immune functions, and the cecum microbiota of weaned Ira white rabbits. A total of 240 weaned 35-day-old Ira white rabbits were randomly allocated to five dietary tre...
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2025-01-01
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| author | Ying-Huan Zhou Xiao-Ping Liu Xiao-Ming Gu Hai-Xuan Lv Yun Yang Zai-Xing Cai Bin Di Chang-Kang Wang Yu-Yun Gao Ling Jin |
| author_facet | Ying-Huan Zhou Xiao-Ping Liu Xiao-Ming Gu Hai-Xuan Lv Yun Yang Zai-Xing Cai Bin Di Chang-Kang Wang Yu-Yun Gao Ling Jin |
| author_sort | Ying-Huan Zhou |
| collection | DOAJ |
| description | This experiment investigated the effects of dietary supplementation with nano-composites of copper and carbon (NCCC) on antioxidants, immune functions, and the cecum microbiota of weaned Ira white rabbits. A total of 240 weaned 35-day-old Ira white rabbits were randomly allocated to five dietary treatments (<i>n</i> = 6 per treatment, each replicate consisted of eight rabbits) that included the control group (CON) with a basal diet, the SAL group with 60 mg/kg salinomycin (SAL) in addition to the basal diet, and the NCCC I, II, III groups, which were supplemented with 50, 100, and 200 mg/kg NCCC, respectively, in addition to the basal diet. The test lasted for 28 d. The results showed that dietary NCCC supplementation increased the liver Cu/Zn-SOD content and up-regulated the gene expression of Cu/Zn-SOD (<i>p</i> < 0.05), while also reducing the content of MDA in the liver and enhancing the antioxidant capacity of Ira white rabbits. Moreover, the NCCC diet supplementation reduced the content of IL-6 and down-regulated the relative expression of <i>IL-6</i> and <i>IL-1β</i> genes in the jejunum of Ira white rabbits (<i>p</i> < 0.05). In addition, the metagenomic analysis of 16 S rRNA showed significant differences in the cecal microbial structure of weaned Ira white rabbits in the NCCC III group compared with the CON, NCCC I, and NCCC II groups (<i>p</i> < 0.05). Firmicutes and Bacteroidetes were the dominant phyla of cecal microorganisms in weaned Ira rabbits in the NCCC diet groups. The dominant genera included unidentified <i>Eubacteriaceae</i>, unclassified <i>Lachnospiraceae</i>, <i>Christensenellaceae</i>, and <i>Ruminococcus</i>. Furthermore, the relative abundance of <i>Ruminococcus</i> in the NCCC I and II groups was lower than that in the CON group in the cecum of Ira white rabbits (<i>p</i> < 0.05). In summary, our results showed that diet supplementation with NCCC could enhance the antioxidant capacity in the liver, alleviate intestinal inflammation, and regulate the structure of intestinal flora, improving the health of Ira white rabbits. |
| format | Article |
| id | doaj-art-6875732d9f1446999fc79291cfbcc2d4 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-6875732d9f1446999fc79291cfbcc2d42025-01-24T13:17:57ZengMDPI AGAnimals2076-26152025-01-0115218410.3390/ani15020184Effects of Dietary Nano-Composite of Copper and Carbon on Antioxidant Capacity, Immunity, and Cecal Microbiota of Weaned Ira White RabbitsYing-Huan Zhou0Xiao-Ping Liu1Xiao-Ming Gu2Hai-Xuan Lv3Yun Yang4Zai-Xing Cai5Bin Di6Chang-Kang Wang7Yu-Yun Gao8Ling Jin9College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaChina National Engineering Research Center of JUNCAO Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaThis experiment investigated the effects of dietary supplementation with nano-composites of copper and carbon (NCCC) on antioxidants, immune functions, and the cecum microbiota of weaned Ira white rabbits. A total of 240 weaned 35-day-old Ira white rabbits were randomly allocated to five dietary treatments (<i>n</i> = 6 per treatment, each replicate consisted of eight rabbits) that included the control group (CON) with a basal diet, the SAL group with 60 mg/kg salinomycin (SAL) in addition to the basal diet, and the NCCC I, II, III groups, which were supplemented with 50, 100, and 200 mg/kg NCCC, respectively, in addition to the basal diet. The test lasted for 28 d. The results showed that dietary NCCC supplementation increased the liver Cu/Zn-SOD content and up-regulated the gene expression of Cu/Zn-SOD (<i>p</i> < 0.05), while also reducing the content of MDA in the liver and enhancing the antioxidant capacity of Ira white rabbits. Moreover, the NCCC diet supplementation reduced the content of IL-6 and down-regulated the relative expression of <i>IL-6</i> and <i>IL-1β</i> genes in the jejunum of Ira white rabbits (<i>p</i> < 0.05). In addition, the metagenomic analysis of 16 S rRNA showed significant differences in the cecal microbial structure of weaned Ira white rabbits in the NCCC III group compared with the CON, NCCC I, and NCCC II groups (<i>p</i> < 0.05). Firmicutes and Bacteroidetes were the dominant phyla of cecal microorganisms in weaned Ira rabbits in the NCCC diet groups. The dominant genera included unidentified <i>Eubacteriaceae</i>, unclassified <i>Lachnospiraceae</i>, <i>Christensenellaceae</i>, and <i>Ruminococcus</i>. Furthermore, the relative abundance of <i>Ruminococcus</i> in the NCCC I and II groups was lower than that in the CON group in the cecum of Ira white rabbits (<i>p</i> < 0.05). In summary, our results showed that diet supplementation with NCCC could enhance the antioxidant capacity in the liver, alleviate intestinal inflammation, and regulate the structure of intestinal flora, improving the health of Ira white rabbits.https://www.mdpi.com/2076-2615/15/2/184NCCCweaning stressliver antioxidantjejunal immunitycecum microorganismsIra white rabbit |
| spellingShingle | Ying-Huan Zhou Xiao-Ping Liu Xiao-Ming Gu Hai-Xuan Lv Yun Yang Zai-Xing Cai Bin Di Chang-Kang Wang Yu-Yun Gao Ling Jin Effects of Dietary Nano-Composite of Copper and Carbon on Antioxidant Capacity, Immunity, and Cecal Microbiota of Weaned Ira White Rabbits Animals NCCC weaning stress liver antioxidant jejunal immunity cecum microorganisms Ira white rabbit |
| title | Effects of Dietary Nano-Composite of Copper and Carbon on Antioxidant Capacity, Immunity, and Cecal Microbiota of Weaned Ira White Rabbits |
| title_full | Effects of Dietary Nano-Composite of Copper and Carbon on Antioxidant Capacity, Immunity, and Cecal Microbiota of Weaned Ira White Rabbits |
| title_fullStr | Effects of Dietary Nano-Composite of Copper and Carbon on Antioxidant Capacity, Immunity, and Cecal Microbiota of Weaned Ira White Rabbits |
| title_full_unstemmed | Effects of Dietary Nano-Composite of Copper and Carbon on Antioxidant Capacity, Immunity, and Cecal Microbiota of Weaned Ira White Rabbits |
| title_short | Effects of Dietary Nano-Composite of Copper and Carbon on Antioxidant Capacity, Immunity, and Cecal Microbiota of Weaned Ira White Rabbits |
| title_sort | effects of dietary nano composite of copper and carbon on antioxidant capacity immunity and cecal microbiota of weaned ira white rabbits |
| topic | NCCC weaning stress liver antioxidant jejunal immunity cecum microorganisms Ira white rabbit |
| url | https://www.mdpi.com/2076-2615/15/2/184 |
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