Distribution of 5-Hydroxymethylcytosine in Different Human Tissues
5-hydroxymethylcytosine (5-hmC) is a modified form of cytosine recently found in mammalians and is believed, like 5-methylcytosine, to also play an important role in switching genes on and off. By utilizing a newly developed 5-hmC immunoassay, we determined the abundance of 5-hmC in human tissues an...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Journal of Nucleic Acids |
| Online Access: | http://dx.doi.org/10.4061/2011/870726 |
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| author | Weiwei Li Min Liu |
| author_facet | Weiwei Li Min Liu |
| author_sort | Weiwei Li |
| collection | DOAJ |
| description | 5-hydroxymethylcytosine (5-hmC) is a modified form of cytosine recently found in mammalians and is believed, like 5-methylcytosine, to also play an important role in switching genes on and off. By utilizing a newly developed 5-hmC immunoassay, we determined the abundance of 5-hmC in human tissues and compared 5-hmC states in normal colorectal tissue and cancerous colorectal tissue. Significant differences of 5-hmC content in different tissues were observed. The percentage of 5-hmC measured is high in brain, liver, kidney and colorectal tissues (0.40–0.65%), while it is relatively low in lung (0.18%) and very low in heart, breast, and placenta (0.05-0.06%). Abundance of 5-hmC in the cancerous colorectal tissues was significantly reduced (0.02–0.06%) compared to that in normal colorectal tissues (0.46–0.57%). Our results showed for the first time that 5-hmC distribution is tissue dependent in human tissues and its abundance could be changed in the diseased states such as colorectal cancer. |
| format | Article |
| id | doaj-art-686d3cfd91ac434a9491ef2b05b91f10 |
| institution | Kabale University |
| issn | 2090-021X |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nucleic Acids |
| spelling | doaj-art-686d3cfd91ac434a9491ef2b05b91f102025-02-03T06:07:47ZengWileyJournal of Nucleic Acids2090-021X2011-01-01201110.4061/2011/870726870726Distribution of 5-Hydroxymethylcytosine in Different Human TissuesWeiwei Li0Min Liu1R&D Division, Epigentek Group Inc., 110 Bi County Boulevard, Suite 122, Farmingdale, NY 11735, USAR&D Division, Epigentek Group Inc., 110 Bi County Boulevard, Suite 122, Farmingdale, NY 11735, USA5-hydroxymethylcytosine (5-hmC) is a modified form of cytosine recently found in mammalians and is believed, like 5-methylcytosine, to also play an important role in switching genes on and off. By utilizing a newly developed 5-hmC immunoassay, we determined the abundance of 5-hmC in human tissues and compared 5-hmC states in normal colorectal tissue and cancerous colorectal tissue. Significant differences of 5-hmC content in different tissues were observed. The percentage of 5-hmC measured is high in brain, liver, kidney and colorectal tissues (0.40–0.65%), while it is relatively low in lung (0.18%) and very low in heart, breast, and placenta (0.05-0.06%). Abundance of 5-hmC in the cancerous colorectal tissues was significantly reduced (0.02–0.06%) compared to that in normal colorectal tissues (0.46–0.57%). Our results showed for the first time that 5-hmC distribution is tissue dependent in human tissues and its abundance could be changed in the diseased states such as colorectal cancer.http://dx.doi.org/10.4061/2011/870726 |
| spellingShingle | Weiwei Li Min Liu Distribution of 5-Hydroxymethylcytosine in Different Human Tissues Journal of Nucleic Acids |
| title | Distribution of 5-Hydroxymethylcytosine in Different Human Tissues |
| title_full | Distribution of 5-Hydroxymethylcytosine in Different Human Tissues |
| title_fullStr | Distribution of 5-Hydroxymethylcytosine in Different Human Tissues |
| title_full_unstemmed | Distribution of 5-Hydroxymethylcytosine in Different Human Tissues |
| title_short | Distribution of 5-Hydroxymethylcytosine in Different Human Tissues |
| title_sort | distribution of 5 hydroxymethylcytosine in different human tissues |
| url | http://dx.doi.org/10.4061/2011/870726 |
| work_keys_str_mv | AT weiweili distributionof5hydroxymethylcytosineindifferenthumantissues AT minliu distributionof5hydroxymethylcytosineindifferenthumantissues |