Dynamic Profile of CD4+ T-Cell-Associated Cytokines/Chemokines following Murine Myocardial Infarction/Reperfusion
CD4+ T-cells play crucial roles in the injured heart. However, the way in which different CD4+ T subtypes function in the myocardial infarction/reperfusion (MI/R) heart is still poorly understood. We aimed to detect the dynamic profile of distinct CD4+ subpopulation-associated cytokines/chemokines b...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2019/9483647 |
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| author | Dongsheng Yuan Jinjun Tie Zhican Xu Guanya Liu Xinyu Ge Zhulin Wang Xumin Zhang Shiyu Gong Gang Liu Qingshu Meng Fang Lin Zhongmin Liu Huimin Fan Xiaohui Zhou |
| author_facet | Dongsheng Yuan Jinjun Tie Zhican Xu Guanya Liu Xinyu Ge Zhulin Wang Xumin Zhang Shiyu Gong Gang Liu Qingshu Meng Fang Lin Zhongmin Liu Huimin Fan Xiaohui Zhou |
| author_sort | Dongsheng Yuan |
| collection | DOAJ |
| description | CD4+ T-cells play crucial roles in the injured heart. However, the way in which different CD4+ T subtypes function in the myocardial infarction/reperfusion (MI/R) heart is still poorly understood. We aimed to detect the dynamic profile of distinct CD4+ subpopulation-associated cytokines/chemokines by relying on a closed-chest acute murine MI/R model. The protein levels of 26 CD4+ T-cell-associated cytokines/chemokines were detected in the heart tissues and serum of mice at day 7 and day 14 post-MI/R or sham surgery. The mRNA levels of IL-4, IL-6, IL-13, IL-27, MIP-1β, MCP-3, and GRO-α were measured in blood mononuclear cells. The protein levels of IL-4, IL-6, IL-13, IL-27, MIP-1β, MCP-3, and GRO-α increased in both injured heart tissues and serum, while IFN-γ, IL-12P70, IL-2, IL-1β, IL-18, TNF-α, IL-5, IL-9, IL-17A, IL-23, IL-10, eotaxin, MIP-1α, RANTES, MCP-1, and MIP-2 increased only in MI/R heart tissues in the day 7 and day 14 groups compared to the sham group. In serum, the IFN-γ, IL-23, and IL-10 levels were downregulated in the MI/R model at both day 7 and day 14 compared to the sham. Compared with the protein expressions in injured heart tissues at day 7, IFN-γ, IL-12P70, IL-2, IL-18, TNF-α, IL-6, IL-4, IL-5, IL-9, IL-17A, IL-23, IL-27, IL-10, eotaxin, IP-10, RANTES, MCP-1, MCP-3, and GRO-α were reduced, while IL-1β and MIP-2 were elevated at day 14. IL-13 and MIP-1β showed higher levels in the MI/R serum at day 14 than at day 7. mRNA levels of IL-4, IL-6, IL-13, and IL-27 were increased in the day 7 group compared to the sham, while MIP-1β, MCP-3, and GRO-α mRNA levels showed no significant difference between the MI/R and sham groups in blood mononuclear cells. Multiple CD4+ T-cell-associated cytokines/chemokines were upregulated in the MI/R hearts at the chronic stage. These results provided important evidence necessary for developing future immunomodulatory therapies after MI/R. |
| format | Article |
| id | doaj-art-6857982d08fe423f81e484e746e432b3 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-6857982d08fe423f81e484e746e432b32025-02-03T01:10:54ZengWileyMediators of Inflammation0962-93511466-18612019-01-01201910.1155/2019/94836479483647Dynamic Profile of CD4+ T-Cell-Associated Cytokines/Chemokines following Murine Myocardial Infarction/ReperfusionDongsheng Yuan0Jinjun Tie1Zhican Xu2Guanya Liu3Xinyu Ge4Zhulin Wang5Xumin Zhang6Shiyu Gong7Gang Liu8Qingshu Meng9Fang Lin10Zhongmin Liu11Huimin Fan12Xiaohui Zhou13Research Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaResearch Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaResearch Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaResearch Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaResearch Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaDepartment of Child Internal Medicine, Shanghai Children’s Medical Center, Shanghai Jiaotong University, Shanghai 200127, ChinaDepartment of Cardiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaDepartment of Cardiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaDepartment of Cardiovascular and Thoracic Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaResearch Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaResearch Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaResearch Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaResearch Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaResearch Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaCD4+ T-cells play crucial roles in the injured heart. However, the way in which different CD4+ T subtypes function in the myocardial infarction/reperfusion (MI/R) heart is still poorly understood. We aimed to detect the dynamic profile of distinct CD4+ subpopulation-associated cytokines/chemokines by relying on a closed-chest acute murine MI/R model. The protein levels of 26 CD4+ T-cell-associated cytokines/chemokines were detected in the heart tissues and serum of mice at day 7 and day 14 post-MI/R or sham surgery. The mRNA levels of IL-4, IL-6, IL-13, IL-27, MIP-1β, MCP-3, and GRO-α were measured in blood mononuclear cells. The protein levels of IL-4, IL-6, IL-13, IL-27, MIP-1β, MCP-3, and GRO-α increased in both injured heart tissues and serum, while IFN-γ, IL-12P70, IL-2, IL-1β, IL-18, TNF-α, IL-5, IL-9, IL-17A, IL-23, IL-10, eotaxin, MIP-1α, RANTES, MCP-1, and MIP-2 increased only in MI/R heart tissues in the day 7 and day 14 groups compared to the sham group. In serum, the IFN-γ, IL-23, and IL-10 levels were downregulated in the MI/R model at both day 7 and day 14 compared to the sham. Compared with the protein expressions in injured heart tissues at day 7, IFN-γ, IL-12P70, IL-2, IL-18, TNF-α, IL-6, IL-4, IL-5, IL-9, IL-17A, IL-23, IL-27, IL-10, eotaxin, IP-10, RANTES, MCP-1, MCP-3, and GRO-α were reduced, while IL-1β and MIP-2 were elevated at day 14. IL-13 and MIP-1β showed higher levels in the MI/R serum at day 14 than at day 7. mRNA levels of IL-4, IL-6, IL-13, and IL-27 were increased in the day 7 group compared to the sham, while MIP-1β, MCP-3, and GRO-α mRNA levels showed no significant difference between the MI/R and sham groups in blood mononuclear cells. Multiple CD4+ T-cell-associated cytokines/chemokines were upregulated in the MI/R hearts at the chronic stage. These results provided important evidence necessary for developing future immunomodulatory therapies after MI/R.http://dx.doi.org/10.1155/2019/9483647 |
| spellingShingle | Dongsheng Yuan Jinjun Tie Zhican Xu Guanya Liu Xinyu Ge Zhulin Wang Xumin Zhang Shiyu Gong Gang Liu Qingshu Meng Fang Lin Zhongmin Liu Huimin Fan Xiaohui Zhou Dynamic Profile of CD4+ T-Cell-Associated Cytokines/Chemokines following Murine Myocardial Infarction/Reperfusion Mediators of Inflammation |
| title | Dynamic Profile of CD4+ T-Cell-Associated Cytokines/Chemokines following Murine Myocardial Infarction/Reperfusion |
| title_full | Dynamic Profile of CD4+ T-Cell-Associated Cytokines/Chemokines following Murine Myocardial Infarction/Reperfusion |
| title_fullStr | Dynamic Profile of CD4+ T-Cell-Associated Cytokines/Chemokines following Murine Myocardial Infarction/Reperfusion |
| title_full_unstemmed | Dynamic Profile of CD4+ T-Cell-Associated Cytokines/Chemokines following Murine Myocardial Infarction/Reperfusion |
| title_short | Dynamic Profile of CD4+ T-Cell-Associated Cytokines/Chemokines following Murine Myocardial Infarction/Reperfusion |
| title_sort | dynamic profile of cd4 t cell associated cytokines chemokines following murine myocardial infarction reperfusion |
| url | http://dx.doi.org/10.1155/2019/9483647 |
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