Applicability of the Filter Paper Technique for Detection of AntifilarialIgG4 Antibodies Using the Bm14 Filariasis CELISA
Demonstration of successful elimination of lymphatic filariasis (LF) in endemic countries requires sensitive diagnostics for accurate definitions of endpoints and future surveillance. There has been interest in complementing available diagnostics with antibody serology testing in children, since neg...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2010/594687 |
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| author | Hayley M. Joseph Wayne Melrose |
| author_facet | Hayley M. Joseph Wayne Melrose |
| author_sort | Hayley M. Joseph |
| collection | DOAJ |
| description | Demonstration of successful elimination of lymphatic filariasis (LF) in endemic countries requires sensitive diagnostics for accurate definitions of endpoints and future surveillance. There has been interest in complementing available diagnostics with antibody serology testing in children, since negative serology would correspond with cessation of LF transmission. The Filariasis CELISA detects antifilarial IgG4 and has favourable results with serum samples but field application requires an easier sampling method. Ninety-four paired plasma and filter paper samples were assayed with promising results. The filter paper method resulted in a sensitivity of 92% and a specificity of 77% when compared to the paired plasma. One hundred and one filter paper samples were assessed for storage effects. Following 10-month storage at −20∘C there was a significant reduction in reactivity (𝑃<.001). Overall the results indicated that filter paper sampling would be a favourable sensitive and specific alternative for blood collection in surveys. |
| format | Article |
| id | doaj-art-683ca5687b014329b49476166c00ed08 |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-683ca5687b014329b49476166c00ed082025-02-03T01:10:45ZengWileyJournal of Parasitology Research2090-00232090-00312010-01-01201010.1155/2010/594687594687Applicability of the Filter Paper Technique for Detection of AntifilarialIgG4 Antibodies Using the Bm14 Filariasis CELISAHayley M. Joseph0Wayne Melrose1Lymphatic Filariasis Support Centre, School of Public Health, Tropical Medicine and Rehabilitation Sciences, James Cook University, Townsville, QLD 4811, AustraliaLymphatic Filariasis Support Centre, School of Public Health, Tropical Medicine and Rehabilitation Sciences, James Cook University, Townsville, QLD 4811, AustraliaDemonstration of successful elimination of lymphatic filariasis (LF) in endemic countries requires sensitive diagnostics for accurate definitions of endpoints and future surveillance. There has been interest in complementing available diagnostics with antibody serology testing in children, since negative serology would correspond with cessation of LF transmission. The Filariasis CELISA detects antifilarial IgG4 and has favourable results with serum samples but field application requires an easier sampling method. Ninety-four paired plasma and filter paper samples were assayed with promising results. The filter paper method resulted in a sensitivity of 92% and a specificity of 77% when compared to the paired plasma. One hundred and one filter paper samples were assessed for storage effects. Following 10-month storage at −20∘C there was a significant reduction in reactivity (𝑃<.001). Overall the results indicated that filter paper sampling would be a favourable sensitive and specific alternative for blood collection in surveys.http://dx.doi.org/10.1155/2010/594687 |
| spellingShingle | Hayley M. Joseph Wayne Melrose Applicability of the Filter Paper Technique for Detection of AntifilarialIgG4 Antibodies Using the Bm14 Filariasis CELISA Journal of Parasitology Research |
| title | Applicability of the Filter Paper Technique for Detection of AntifilarialIgG4 Antibodies Using the Bm14 Filariasis CELISA |
| title_full | Applicability of the Filter Paper Technique for Detection of AntifilarialIgG4 Antibodies Using the Bm14 Filariasis CELISA |
| title_fullStr | Applicability of the Filter Paper Technique for Detection of AntifilarialIgG4 Antibodies Using the Bm14 Filariasis CELISA |
| title_full_unstemmed | Applicability of the Filter Paper Technique for Detection of AntifilarialIgG4 Antibodies Using the Bm14 Filariasis CELISA |
| title_short | Applicability of the Filter Paper Technique for Detection of AntifilarialIgG4 Antibodies Using the Bm14 Filariasis CELISA |
| title_sort | applicability of the filter paper technique for detection of antifilarialigg4 antibodies using the bm14 filariasis celisa |
| url | http://dx.doi.org/10.1155/2010/594687 |
| work_keys_str_mv | AT hayleymjoseph applicabilityofthefilterpapertechniquefordetectionofantifilarialigg4antibodiesusingthebm14filariasiscelisa AT waynemelrose applicabilityofthefilterpapertechniquefordetectionofantifilarialigg4antibodiesusingthebm14filariasiscelisa |