A multiprotein regulatory module, MED16–MBR1&2, controls MED25 homeostasis during jasmonate signaling
Abstract Mediator25 (MED25) has been ascribed as a signal-processing and -integrating center that controls jasmonate (JA)-induced and MYC2-dependent transcriptional output. A better understanding of the regulation of MED25 stability will undoubtedly advance our knowledge of the precise regulation of...
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| Format: | Article |
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Nature Portfolio
2025-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-56041-3 |
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| author | Fangming Wu Chuanlong Sun Ziying Zhu Lei Deng Feifei Yu Qi Xie Chuanyou Li |
| author_facet | Fangming Wu Chuanlong Sun Ziying Zhu Lei Deng Feifei Yu Qi Xie Chuanyou Li |
| author_sort | Fangming Wu |
| collection | DOAJ |
| description | Abstract Mediator25 (MED25) has been ascribed as a signal-processing and -integrating center that controls jasmonate (JA)-induced and MYC2-dependent transcriptional output. A better understanding of the regulation of MED25 stability will undoubtedly advance our knowledge of the precise regulation of JA signaling-related transcriptional output. Here, we report that Arabidopsis MED16 activates JA-responsive gene expression by promoting MED25 stability. Conversely, two homologous E3 ubiquitin ligases, MED25-BINDING RING-H2 PROTEIN1 (MBR1) and MBR2, negatively regulate JA-responsive gene expression by promoting MED25 degradation. MED16 competes with MBR1&2 to bind to the von Willebrand Factor A (vWF-A) domain of MED25, thereby antagonizing the MBR1&2-mediated degradation of MED25 in vivo. In addition, we show that MED16 promotes hormone-induced interactions between MYC2 and MED25, leading to the activation of JA-responsive gene expression. Collectively, our findings reveal a multiprotein regulatory module that robustly and tightly maintains MED25 homeostasis, which determines the strength of the transcriptional output of JA signaling. |
| format | Article |
| id | doaj-art-682f8ba5165745caae442755f3840b11 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-682f8ba5165745caae442755f3840b112025-01-19T12:30:23ZengNature PortfolioNature Communications2041-17232025-01-0116111610.1038/s41467-025-56041-3A multiprotein regulatory module, MED16–MBR1&2, controls MED25 homeostasis during jasmonate signalingFangming Wu0Chuanlong Sun1Ziying Zhu2Lei Deng3Feifei Yu4Qi Xie5Chuanyou Li6Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesTaishan Academy of Tomato Innovation, Shandong Agricultural UniversityKey Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesTaishan Academy of Tomato Innovation, Shandong Agricultural UniversityCollege of Grassland Science and Technology, China Agricultural UniversityKey Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesKey Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesAbstract Mediator25 (MED25) has been ascribed as a signal-processing and -integrating center that controls jasmonate (JA)-induced and MYC2-dependent transcriptional output. A better understanding of the regulation of MED25 stability will undoubtedly advance our knowledge of the precise regulation of JA signaling-related transcriptional output. Here, we report that Arabidopsis MED16 activates JA-responsive gene expression by promoting MED25 stability. Conversely, two homologous E3 ubiquitin ligases, MED25-BINDING RING-H2 PROTEIN1 (MBR1) and MBR2, negatively regulate JA-responsive gene expression by promoting MED25 degradation. MED16 competes with MBR1&2 to bind to the von Willebrand Factor A (vWF-A) domain of MED25, thereby antagonizing the MBR1&2-mediated degradation of MED25 in vivo. In addition, we show that MED16 promotes hormone-induced interactions between MYC2 and MED25, leading to the activation of JA-responsive gene expression. Collectively, our findings reveal a multiprotein regulatory module that robustly and tightly maintains MED25 homeostasis, which determines the strength of the transcriptional output of JA signaling.https://doi.org/10.1038/s41467-025-56041-3 |
| spellingShingle | Fangming Wu Chuanlong Sun Ziying Zhu Lei Deng Feifei Yu Qi Xie Chuanyou Li A multiprotein regulatory module, MED16–MBR1&2, controls MED25 homeostasis during jasmonate signaling Nature Communications |
| title | A multiprotein regulatory module, MED16–MBR1&2, controls MED25 homeostasis during jasmonate signaling |
| title_full | A multiprotein regulatory module, MED16–MBR1&2, controls MED25 homeostasis during jasmonate signaling |
| title_fullStr | A multiprotein regulatory module, MED16–MBR1&2, controls MED25 homeostasis during jasmonate signaling |
| title_full_unstemmed | A multiprotein regulatory module, MED16–MBR1&2, controls MED25 homeostasis during jasmonate signaling |
| title_short | A multiprotein regulatory module, MED16–MBR1&2, controls MED25 homeostasis during jasmonate signaling |
| title_sort | multiprotein regulatory module med16 mbr1 2 controls med25 homeostasis during jasmonate signaling |
| url | https://doi.org/10.1038/s41467-025-56041-3 |
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