Transcriptome-Based Modeling Reveals that Oxidative Stress Induces Modulation of the AtfA-Dependent Signaling Networks in Aspergillus nidulans
To better understand the molecular functions of the master stress-response regulator AtfA in Aspergillus nidulans, transcriptomic analyses of the atfA null mutant and the appropriate control strains exposed to menadione sodium bisulfite- (MSB-), t-butylhydroperoxide- and diamide-induced oxidative st...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2017/6923849 |
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| author | Erzsébet Orosz Károly Antal Zoltán Gazdag Zsuzsa Szabó Kap-Hoon Han Jae-Hyuk Yu István Pócsi Tamás Emri |
| author_facet | Erzsébet Orosz Károly Antal Zoltán Gazdag Zsuzsa Szabó Kap-Hoon Han Jae-Hyuk Yu István Pócsi Tamás Emri |
| author_sort | Erzsébet Orosz |
| collection | DOAJ |
| description | To better understand the molecular functions of the master stress-response regulator AtfA in Aspergillus nidulans, transcriptomic analyses of the atfA null mutant and the appropriate control strains exposed to menadione sodium bisulfite- (MSB-), t-butylhydroperoxide- and diamide-induced oxidative stresses were performed. Several elements of oxidative stress response were differentially expressed. Many of them, including the downregulation of the mitotic cell cycle, as the MSB stress-specific upregulation of FeS cluster assembly and the MSB stress-specific downregulation of nitrate reduction, tricarboxylic acid cycle, and ER to Golgi vesicle-mediated transport, showed AtfA dependence. To elucidate the potential global regulatory role of AtfA governing expression of a high number of genes with very versatile biological functions, we devised a model based on the comprehensive transcriptomic data. Our model suggests that an important function of AtfA is to modulate the transduction of stress signals. Although it may regulate directly only a limited number of genes, these include elements of the signaling network, for example, members of the two-component signal transduction systems. AtfA acts in a stress-specific manner, which may increase further the number and diversity of AtfA-dependent genes. Our model sheds light on the versatility of the physiological functions of AtfA and its orthologs in fungi. |
| format | Article |
| id | doaj-art-681563e559fa4564b603a6aa68275148 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-681563e559fa4564b603a6aa682751482025-02-03T01:09:38ZengWileyInternational Journal of Genomics2314-436X2314-43782017-01-01201710.1155/2017/69238496923849Transcriptome-Based Modeling Reveals that Oxidative Stress Induces Modulation of the AtfA-Dependent Signaling Networks in Aspergillus nidulansErzsébet Orosz0Károly Antal1Zoltán Gazdag2Zsuzsa Szabó3Kap-Hoon Han4Jae-Hyuk Yu5István Pócsi6Tamás Emri7Department of Biotechnology and Microbiology, Faculty of Science and Technology, University of Debrecen, Debrecen, P.O. Box 63 H-4010, HungaryDepartment of Zoology, Faculty of Sciences, Eszterházy Károly University, Eger, Eszterházy tér 1 H-3300, HungaryDepartment of General and Environmental Microbiology, Faculty of Sciences, University of Pécs, Pécs, P. O. Box 266 H-7601, HungaryDepartment of Biotechnology and Microbiology, Faculty of Science and Technology, University of Debrecen, Debrecen, P.O. Box 63 H-4010, HungaryDepartment of Pharmaceutical Engineering, Woosuk University, Wanju 565-701, Republic of KoreaDepartment of Bacteriology, University of Wisconsin, 1550 Linden Dr., Madison, WI 53706, USADepartment of Biotechnology and Microbiology, Faculty of Science and Technology, University of Debrecen, Debrecen, P.O. Box 63 H-4010, HungaryDepartment of Biotechnology and Microbiology, Faculty of Science and Technology, University of Debrecen, Debrecen, P.O. Box 63 H-4010, HungaryTo better understand the molecular functions of the master stress-response regulator AtfA in Aspergillus nidulans, transcriptomic analyses of the atfA null mutant and the appropriate control strains exposed to menadione sodium bisulfite- (MSB-), t-butylhydroperoxide- and diamide-induced oxidative stresses were performed. Several elements of oxidative stress response were differentially expressed. Many of them, including the downregulation of the mitotic cell cycle, as the MSB stress-specific upregulation of FeS cluster assembly and the MSB stress-specific downregulation of nitrate reduction, tricarboxylic acid cycle, and ER to Golgi vesicle-mediated transport, showed AtfA dependence. To elucidate the potential global regulatory role of AtfA governing expression of a high number of genes with very versatile biological functions, we devised a model based on the comprehensive transcriptomic data. Our model suggests that an important function of AtfA is to modulate the transduction of stress signals. Although it may regulate directly only a limited number of genes, these include elements of the signaling network, for example, members of the two-component signal transduction systems. AtfA acts in a stress-specific manner, which may increase further the number and diversity of AtfA-dependent genes. Our model sheds light on the versatility of the physiological functions of AtfA and its orthologs in fungi.http://dx.doi.org/10.1155/2017/6923849 |
| spellingShingle | Erzsébet Orosz Károly Antal Zoltán Gazdag Zsuzsa Szabó Kap-Hoon Han Jae-Hyuk Yu István Pócsi Tamás Emri Transcriptome-Based Modeling Reveals that Oxidative Stress Induces Modulation of the AtfA-Dependent Signaling Networks in Aspergillus nidulans International Journal of Genomics |
| title | Transcriptome-Based Modeling Reveals that Oxidative Stress Induces Modulation of the AtfA-Dependent Signaling Networks in Aspergillus nidulans |
| title_full | Transcriptome-Based Modeling Reveals that Oxidative Stress Induces Modulation of the AtfA-Dependent Signaling Networks in Aspergillus nidulans |
| title_fullStr | Transcriptome-Based Modeling Reveals that Oxidative Stress Induces Modulation of the AtfA-Dependent Signaling Networks in Aspergillus nidulans |
| title_full_unstemmed | Transcriptome-Based Modeling Reveals that Oxidative Stress Induces Modulation of the AtfA-Dependent Signaling Networks in Aspergillus nidulans |
| title_short | Transcriptome-Based Modeling Reveals that Oxidative Stress Induces Modulation of the AtfA-Dependent Signaling Networks in Aspergillus nidulans |
| title_sort | transcriptome based modeling reveals that oxidative stress induces modulation of the atfa dependent signaling networks in aspergillus nidulans |
| url | http://dx.doi.org/10.1155/2017/6923849 |
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