Spermatic RXFP2 expression levels and seminal INSL3 concentrations among beef bull ejaculates with different levels of sperm morphological normality
The objective of this study was to examine the relationship between the insulin-like peptide 3 (INSL3) receptor (RXFP2) expression levels on spermatozoa and INSL3 concentrations in the seminal plasma of fresh semen from beef bulls with different levels of sperm morphological normality. Ejaculates (n...
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The Society for Reproduction and Development
2024-12-01
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| Series: | The Journal of Reproduction and Development |
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| Online Access: | https://www.jstage.jst.go.jp/article/jrd/71/1/71_2024-072/_pdf/-char/en |
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| author | Hewage Dilhan Anuradha WIMALARATHNE Kenta ARASHI Fumiyuki IWAKI Mitsuhiro SAKASE DURITAHALA Hiroshi HARAYAMA Noritoshi KAWATE |
| author_facet | Hewage Dilhan Anuradha WIMALARATHNE Kenta ARASHI Fumiyuki IWAKI Mitsuhiro SAKASE DURITAHALA Hiroshi HARAYAMA Noritoshi KAWATE |
| author_sort | Hewage Dilhan Anuradha WIMALARATHNE |
| collection | DOAJ |
| description | The objective of this study was to examine the relationship between the insulin-like peptide 3 (INSL3) receptor (RXFP2) expression levels on spermatozoa and INSL3 concentrations in the seminal plasma of fresh semen from beef bulls with different levels of sperm morphological normality. Ejaculates (n = 44) were collected from 21 yearling Japanese Black beef bulls and categorized into three groups based on the levels of sperm morphological normality: High (normal morphology ≥ 80%; n = 23), Mid (< 80% & ≥ 65%; n = 10) and Low (< 65%; n = 11). Immunofluorescence was used to determine the localization and expression levels of RXFP2 in spermatozoa. Sperm RXFP2 was detected in the principal and equatorial segments of the acrosomal region, postacrosomal region, and neck in all groups. The levels of RXFP2 in the acrosomal principal segment, postacrosomal region, and neck in the Low group were significantly lower than those in the High and Mid groups, and those in the equatorial segment tended to be lower than those in the High group. The total level of RXFP2 in the Low group was also significantly reduced compared with that in the other two groups. Seminal plasma INSL3 concentrations were significantly higher in the Low group than in the other two groups, whereas testosterone levels did not differ significantly between the groups. In conclusion, RXFP2 levels were reduced in the sperm head and neck in bovine semen, with a lower level of sperm morphological normality, suggesting possible associations between increased sperm deformity and INSL3 receptor reduction. Higher seminal INSL3 concentrations in abnormal semen are probably related to fewer INSL3 receptors in spermatozoa. |
| format | Article |
| id | doaj-art-67fb8729888c4e1cab720fcfd1105e4a |
| institution | Kabale University |
| issn | 0916-8818 1348-4400 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj-art-67fb8729888c4e1cab720fcfd1105e4a2025-02-04T23:41:35ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002024-12-01711354010.1262/jrd.2024-072jrdSpermatic RXFP2 expression levels and seminal INSL3 concentrations among beef bull ejaculates with different levels of sperm morphological normalityHewage Dilhan Anuradha WIMALARATHNE0Kenta ARASHI1Fumiyuki IWAKI2Mitsuhiro SAKASE3DURITAHALA4Hiroshi HARAYAMA5Noritoshi KAWATE6Laboratory of Theriogenology, Graduate School of Veterinary Science, Osaka Metropolitan University, Osaka 598-8531, JapanLaboratory of Theriogenology, Graduate School of Veterinary Science, Osaka Metropolitan University, Osaka 598-8531, JapanHokubu Agricultural Technology Institute, Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries, Hyogo 669-5254, JapanLivestock Technology Institute, Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries, Hyogo 679-0198, JapanLaboratory of Reproductive Biology, Graduate School of Agricultural Science, Kobe University, Hyogo 657-8501, JapanLaboratory of Reproductive Biology, Graduate School of Agricultural Science, Kobe University, Hyogo 657-8501, JapanLaboratory of Theriogenology, Graduate School of Veterinary Science, Osaka Metropolitan University, Osaka 598-8531, JapanThe objective of this study was to examine the relationship between the insulin-like peptide 3 (INSL3) receptor (RXFP2) expression levels on spermatozoa and INSL3 concentrations in the seminal plasma of fresh semen from beef bulls with different levels of sperm morphological normality. Ejaculates (n = 44) were collected from 21 yearling Japanese Black beef bulls and categorized into three groups based on the levels of sperm morphological normality: High (normal morphology ≥ 80%; n = 23), Mid (< 80% & ≥ 65%; n = 10) and Low (< 65%; n = 11). Immunofluorescence was used to determine the localization and expression levels of RXFP2 in spermatozoa. Sperm RXFP2 was detected in the principal and equatorial segments of the acrosomal region, postacrosomal region, and neck in all groups. The levels of RXFP2 in the acrosomal principal segment, postacrosomal region, and neck in the Low group were significantly lower than those in the High and Mid groups, and those in the equatorial segment tended to be lower than those in the High group. The total level of RXFP2 in the Low group was also significantly reduced compared with that in the other two groups. Seminal plasma INSL3 concentrations were significantly higher in the Low group than in the other two groups, whereas testosterone levels did not differ significantly between the groups. In conclusion, RXFP2 levels were reduced in the sperm head and neck in bovine semen, with a lower level of sperm morphological normality, suggesting possible associations between increased sperm deformity and INSL3 receptor reduction. Higher seminal INSL3 concentrations in abnormal semen are probably related to fewer INSL3 receptors in spermatozoa.https://www.jstage.jst.go.jp/article/jrd/71/1/71_2024-072/_pdf/-char/enbeef bullinsulin-like peptide 3 (insl3)relaxin family peptide receptor 2 (rxfp2)seminal plasmasperm morphology |
| spellingShingle | Hewage Dilhan Anuradha WIMALARATHNE Kenta ARASHI Fumiyuki IWAKI Mitsuhiro SAKASE DURITAHALA Hiroshi HARAYAMA Noritoshi KAWATE Spermatic RXFP2 expression levels and seminal INSL3 concentrations among beef bull ejaculates with different levels of sperm morphological normality The Journal of Reproduction and Development beef bull insulin-like peptide 3 (insl3) relaxin family peptide receptor 2 (rxfp2) seminal plasma sperm morphology |
| title | Spermatic RXFP2 expression levels and seminal INSL3 concentrations among beef bull ejaculates with different levels of sperm morphological normality |
| title_full | Spermatic RXFP2 expression levels and seminal INSL3 concentrations among beef bull ejaculates with different levels of sperm morphological normality |
| title_fullStr | Spermatic RXFP2 expression levels and seminal INSL3 concentrations among beef bull ejaculates with different levels of sperm morphological normality |
| title_full_unstemmed | Spermatic RXFP2 expression levels and seminal INSL3 concentrations among beef bull ejaculates with different levels of sperm morphological normality |
| title_short | Spermatic RXFP2 expression levels and seminal INSL3 concentrations among beef bull ejaculates with different levels of sperm morphological normality |
| title_sort | spermatic rxfp2 expression levels and seminal insl3 concentrations among beef bull ejaculates with different levels of sperm morphological normality |
| topic | beef bull insulin-like peptide 3 (insl3) relaxin family peptide receptor 2 (rxfp2) seminal plasma sperm morphology |
| url | https://www.jstage.jst.go.jp/article/jrd/71/1/71_2024-072/_pdf/-char/en |
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