METTL3-Mediated m6A RNA Modification Regulates Corneal Injury Repair
Limbal stem cells are essential for continuous corneal regeneration and injury repair. METTL3-catalyzed N6-methyladenosine (m6A) mRNA modifications are involved in many biological processes and play a specific role in stem cell regeneration, while the role of m6A modifications in corneal injury repa...
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/5512153 |
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| author | Yarong Dai Maosheng Cheng Siyan Zhang Rongsong Ling Jieqi Wen Yifan Cheng Boxuan Huang Jinrong Li Caifeng Dai Shiqing Mao Shuibin Lin Huangxuan Shen Yizhou Jiang |
| author_facet | Yarong Dai Maosheng Cheng Siyan Zhang Rongsong Ling Jieqi Wen Yifan Cheng Boxuan Huang Jinrong Li Caifeng Dai Shiqing Mao Shuibin Lin Huangxuan Shen Yizhou Jiang |
| author_sort | Yarong Dai |
| collection | DOAJ |
| description | Limbal stem cells are essential for continuous corneal regeneration and injury repair. METTL3-catalyzed N6-methyladenosine (m6A) mRNA modifications are involved in many biological processes and play a specific role in stem cell regeneration, while the role of m6A modifications in corneal injury repair remains unknown. In this study, we generated a limbal stem cell-specific METTL3 knockout mouse model and studied the role of m6A in repairing corneal injury caused by alkali burn. The results showed that METTL3 knockout in the limbal stem cells promotes the in vivo cell proliferation and migration, leading to the fast repair of corneal injury. In addition, m6A modification profiling identified stem cell regulatory factors AHNAK and DDIT4 as m6A targets. Our study reveals the essential functions of m6A RNA modification in regulating injury repair and provides novel insights for clinical therapy of corneal diseases. |
| format | Article |
| id | doaj-art-67fb4ff9e993473cb06ce8a1f0056837 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-67fb4ff9e993473cb06ce8a1f00568372025-08-20T03:33:54ZengWileyStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/55121535512153METTL3-Mediated m6A RNA Modification Regulates Corneal Injury RepairYarong Dai0Maosheng Cheng1Siyan Zhang2Rongsong Ling3Jieqi Wen4Yifan Cheng5Boxuan Huang6Jinrong Li7Caifeng Dai8Shiqing Mao9Shuibin Lin10Huangxuan Shen11Yizhou Jiang12Institute for Advanced Study, Shenzhen University, 518067, ChinaAnhui Medical University, 230032, ChinaInstitute for Advanced Study, Shenzhen University, 518067, ChinaInstitute for Advanced Study, Shenzhen University, 518067, ChinaInstitute for Advanced Study, Shenzhen University, 518067, ChinaInstitute for Advanced Study, Shenzhen University, 518067, ChinaInstitute for Advanced Study, Shenzhen University, 518067, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, 510064, ChinaCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, 250012, ChinaInstitute for Advanced Study, Shenzhen University, 518067, ChinaCenter for Translational Medicine, Precision Medicine Institute, The First Affiliated Hospital, Sun Yat-sen University, 510064, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, 510064, ChinaInstitute for Advanced Study, Shenzhen University, 518067, ChinaLimbal stem cells are essential for continuous corneal regeneration and injury repair. METTL3-catalyzed N6-methyladenosine (m6A) mRNA modifications are involved in many biological processes and play a specific role in stem cell regeneration, while the role of m6A modifications in corneal injury repair remains unknown. In this study, we generated a limbal stem cell-specific METTL3 knockout mouse model and studied the role of m6A in repairing corneal injury caused by alkali burn. The results showed that METTL3 knockout in the limbal stem cells promotes the in vivo cell proliferation and migration, leading to the fast repair of corneal injury. In addition, m6A modification profiling identified stem cell regulatory factors AHNAK and DDIT4 as m6A targets. Our study reveals the essential functions of m6A RNA modification in regulating injury repair and provides novel insights for clinical therapy of corneal diseases.http://dx.doi.org/10.1155/2021/5512153 |
| spellingShingle | Yarong Dai Maosheng Cheng Siyan Zhang Rongsong Ling Jieqi Wen Yifan Cheng Boxuan Huang Jinrong Li Caifeng Dai Shiqing Mao Shuibin Lin Huangxuan Shen Yizhou Jiang METTL3-Mediated m6A RNA Modification Regulates Corneal Injury Repair Stem Cells International |
| title | METTL3-Mediated m6A RNA Modification Regulates Corneal Injury Repair |
| title_full | METTL3-Mediated m6A RNA Modification Regulates Corneal Injury Repair |
| title_fullStr | METTL3-Mediated m6A RNA Modification Regulates Corneal Injury Repair |
| title_full_unstemmed | METTL3-Mediated m6A RNA Modification Regulates Corneal Injury Repair |
| title_short | METTL3-Mediated m6A RNA Modification Regulates Corneal Injury Repair |
| title_sort | mettl3 mediated m6a rna modification regulates corneal injury repair |
| url | http://dx.doi.org/10.1155/2021/5512153 |
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