Activation of CD147 with Cyclophilin A Induces the Expression of IFITM1 through ERK and PI3K in THP-1 Cells
CD147, as a receptor for Cyclophilins, is a multifunctional transmembrane glycoprotein. In order to identify genes that are induced by activation of CD147, THP-1 cells were stimulated with Cyclophilin A and differentially expressed genes were detected using PCR-based analysis. Interferon-induced tra...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2010/821940 |
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| _version_ | 1832562472005926912 |
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| author | Ju-Young Kim Ho Kim Kyoungho Suk Won-Ha Lee |
| author_facet | Ju-Young Kim Ho Kim Kyoungho Suk Won-Ha Lee |
| author_sort | Ju-Young Kim |
| collection | DOAJ |
| description | CD147, as a receptor for Cyclophilins, is a multifunctional transmembrane glycoprotein. In order to identify genes that are induced by activation of CD147, THP-1 cells were stimulated with Cyclophilin A and differentially expressed genes were detected using PCR-based analysis. Interferon-induced transmembrane 1 (IFITM1) was detected to be induced and it was confirmed by RT-PCR and Western blot analysis. CD147-induced expression of IFITM1 was blocked by inhibitors of ERK, PI3K, or NF-κB, but not by inhibitors of p38, JNK, or PKC. IFITM1 appears to mediate inflammatory activation of THP-1 cells since cross-linking of IFITM1 with specific monoclonal antibody against it induced the expression of proinflammatory mediators such as IL-8 and MMP-9. These data indicate that IFITM1 is one of the pro-inflammatory mediators that are induced by signaling initiated by the activation of CD147 in macrophages and activation of ERK, PI3K, and NF-κB is required for the expression of IFITM1. |
| format | Article |
| id | doaj-art-67d6094feb554589ad0737272da8d730 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-67d6094feb554589ad0737272da8d7302025-02-03T01:22:33ZengWileyMediators of Inflammation0962-93511466-18612010-01-01201010.1155/2010/821940821940Activation of CD147 with Cyclophilin A Induces the Expression of IFITM1 through ERK and PI3K in THP-1 CellsJu-Young Kim0Ho Kim1Kyoungho Suk2Won-Ha Lee3School of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of KoreaSchool of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of KoreaDepartment of Pharmacology, School of Medicine, Kyungpook National University, Daegu 702-701, Republic of KoreaSchool of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of KoreaCD147, as a receptor for Cyclophilins, is a multifunctional transmembrane glycoprotein. In order to identify genes that are induced by activation of CD147, THP-1 cells were stimulated with Cyclophilin A and differentially expressed genes were detected using PCR-based analysis. Interferon-induced transmembrane 1 (IFITM1) was detected to be induced and it was confirmed by RT-PCR and Western blot analysis. CD147-induced expression of IFITM1 was blocked by inhibitors of ERK, PI3K, or NF-κB, but not by inhibitors of p38, JNK, or PKC. IFITM1 appears to mediate inflammatory activation of THP-1 cells since cross-linking of IFITM1 with specific monoclonal antibody against it induced the expression of proinflammatory mediators such as IL-8 and MMP-9. These data indicate that IFITM1 is one of the pro-inflammatory mediators that are induced by signaling initiated by the activation of CD147 in macrophages and activation of ERK, PI3K, and NF-κB is required for the expression of IFITM1.http://dx.doi.org/10.1155/2010/821940 |
| spellingShingle | Ju-Young Kim Ho Kim Kyoungho Suk Won-Ha Lee Activation of CD147 with Cyclophilin A Induces the Expression of IFITM1 through ERK and PI3K in THP-1 Cells Mediators of Inflammation |
| title | Activation of CD147 with Cyclophilin A Induces the Expression of IFITM1 through ERK and PI3K in THP-1 Cells |
| title_full | Activation of CD147 with Cyclophilin A Induces the Expression of IFITM1 through ERK and PI3K in THP-1 Cells |
| title_fullStr | Activation of CD147 with Cyclophilin A Induces the Expression of IFITM1 through ERK and PI3K in THP-1 Cells |
| title_full_unstemmed | Activation of CD147 with Cyclophilin A Induces the Expression of IFITM1 through ERK and PI3K in THP-1 Cells |
| title_short | Activation of CD147 with Cyclophilin A Induces the Expression of IFITM1 through ERK and PI3K in THP-1 Cells |
| title_sort | activation of cd147 with cyclophilin a induces the expression of ifitm1 through erk and pi3k in thp 1 cells |
| url | http://dx.doi.org/10.1155/2010/821940 |
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