Imaging cell spheroid clusters: An MRI protocol for non-invasive standardized characterization
Over the past decade, significant progress has been made in the utilization of three-dimensional cell cultures in the form of spheroids as a bridge between in vitro and in vivo models. This is contributed by natural cell-cell interactions that occur within spheroids, leading to the subsequent develo...
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Elsevier
2025-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025001835 |
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| author | Rebecca Wißmann Petros Martirosian Marina Danalache Gerd Grözinger Fritz Schick Stefanie Elser |
| author_facet | Rebecca Wißmann Petros Martirosian Marina Danalache Gerd Grözinger Fritz Schick Stefanie Elser |
| author_sort | Rebecca Wißmann |
| collection | DOAJ |
| description | Over the past decade, significant progress has been made in the utilization of three-dimensional cell cultures in the form of spheroids as a bridge between in vitro and in vivo models. This is contributed by natural cell-cell interactions that occur within spheroids, leading to the subsequent development of extracellular matrix. The assessment of cell spheroids with conventional microscopy is destructive, requiring sectioning that damages their micro-structures. To address these issues, we developed and propose a non-invasive approach using magnetic resonance imaging (MRI). Despite its limited spatial resolution, this method adeptly reveals information about the composition and vitality of stem cell and cancer spheroids and their micro-environment in a non-invasive manner. This work reports on the development of an MRI-compatible setup for culturing cell spheroids, tailored for use with standard 3 T whole-body MRI systems. Systematic studies with different cell types show the potential of the proposed approach for simultaneous actuation and visualization of cell spheroids, with potential across a broad spectrum of applications. |
| format | Article |
| id | doaj-art-67d00e2f336d401284efb187d1eaca85 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-67d00e2f336d401284efb187d1eaca852025-02-02T05:28:14ZengElsevierHeliyon2405-84402025-01-01112e41803Imaging cell spheroid clusters: An MRI protocol for non-invasive standardized characterizationRebecca Wißmann0Petros Martirosian1Marina Danalache2Gerd Grözinger3Fritz Schick4Stefanie Elser5Department of Diagnostic and Interventional Radiology, University Hospital of Tübingen, D-72076, Tübingen, Germany; Corresponding author. University Hospital Tübingen, Department of Diagnostic and Interventional Radiology, Hoppe-Seyler-Str.3, 72076, Tübingen, Germany.Rebecca.Wissmann@med.uni-tuebingen.deDepartment of Diagnostic and Interventional Radiology, University Hospital of Tübingen, D-72076, Tübingen, Germany; Section on Experimental Radiology, Department of Diagnostic and Interventional Radiology, Tübingen University Hospital, Tübingen, GermanyDepartment of Orthopaedic Surgery, University Hospital of Tübingen, D-72076, Tübingen, GermanyDepartment of Diagnostic and Interventional Radiology, University Hospital of Tübingen, D-72076, Tübingen, GermanyDepartment of Diagnostic and Interventional Radiology, University Hospital of Tübingen, D-72076, Tübingen, Germany; Section on Experimental Radiology, Department of Diagnostic and Interventional Radiology, Tübingen University Hospital, Tübingen, GermanyDepartment of Diagnostic and Interventional Radiology, University Hospital of Tübingen, D-72076, Tübingen, GermanyOver the past decade, significant progress has been made in the utilization of three-dimensional cell cultures in the form of spheroids as a bridge between in vitro and in vivo models. This is contributed by natural cell-cell interactions that occur within spheroids, leading to the subsequent development of extracellular matrix. The assessment of cell spheroids with conventional microscopy is destructive, requiring sectioning that damages their micro-structures. To address these issues, we developed and propose a non-invasive approach using magnetic resonance imaging (MRI). Despite its limited spatial resolution, this method adeptly reveals information about the composition and vitality of stem cell and cancer spheroids and their micro-environment in a non-invasive manner. This work reports on the development of an MRI-compatible setup for culturing cell spheroids, tailored for use with standard 3 T whole-body MRI systems. Systematic studies with different cell types show the potential of the proposed approach for simultaneous actuation and visualization of cell spheroids, with potential across a broad spectrum of applications.http://www.sciencedirect.com/science/article/pii/S2405844025001835SpheroidsMagnetic resonance imagingVisualization3D cultureTumor modelRegenerative medicine |
| spellingShingle | Rebecca Wißmann Petros Martirosian Marina Danalache Gerd Grözinger Fritz Schick Stefanie Elser Imaging cell spheroid clusters: An MRI protocol for non-invasive standardized characterization Heliyon Spheroids Magnetic resonance imaging Visualization 3D culture Tumor model Regenerative medicine |
| title | Imaging cell spheroid clusters: An MRI protocol for non-invasive standardized characterization |
| title_full | Imaging cell spheroid clusters: An MRI protocol for non-invasive standardized characterization |
| title_fullStr | Imaging cell spheroid clusters: An MRI protocol for non-invasive standardized characterization |
| title_full_unstemmed | Imaging cell spheroid clusters: An MRI protocol for non-invasive standardized characterization |
| title_short | Imaging cell spheroid clusters: An MRI protocol for non-invasive standardized characterization |
| title_sort | imaging cell spheroid clusters an mri protocol for non invasive standardized characterization |
| topic | Spheroids Magnetic resonance imaging Visualization 3D culture Tumor model Regenerative medicine |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025001835 |
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