Structural Characterization of Polygonum divaricatum L. Polysaccharide and Its Inhibitory Effect on Lipopolysaccharide-Induced Inflammation in RAW264.7 Cells
Objective: To explore the in vitro anti-inflammatory activity and action mechanism of a polysaccharide extracted from Polygonum divaricatum L. (PSPDL). Methods: The structure of PSPDL was characterized using high-performance gel permeation chromatography (HPGPC), high-performance liquid chromatograp...
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China Food Publishing Company
2024-12-01
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| Series: | Shipin Kexue |
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| author | CUI Yanyan, YUAN Yongxu, GUO Mingkun, HE Wenbing, LI Ming, PEI Shichun, LI Dajun |
| author_facet | CUI Yanyan, YUAN Yongxu, GUO Mingkun, HE Wenbing, LI Ming, PEI Shichun, LI Dajun |
| author_sort | CUI Yanyan, YUAN Yongxu, GUO Mingkun, HE Wenbing, LI Ming, PEI Shichun, LI Dajun |
| collection | DOAJ |
| description | Objective: To explore the in vitro anti-inflammatory activity and action mechanism of a polysaccharide extracted from Polygonum divaricatum L. (PSPDL). Methods: The structure of PSPDL was characterized using high-performance gel permeation chromatography (HPGPC), high-performance liquid chromatography (HPLC), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and nuclear magnetic resonance spectroscopy (NMR). The in vitro anti-inflammatory activity and action mechanism of PSPDL were investigated in lipopolysaccharide (LPS)-induced RAW264.7 cells. Results: PSPDL had a relative molecular mass of 59.475 kDa and consisted mainly of mannose (Man), rhamnose (Rha), glucuronic acid (GlcA), galacturonic acid (GalA), glucose (Glc), galactose (Gal), and arabinose (Ara), with a molar ratio of 1.69:4.95:1.04:21.79:19.01:31.68:19.84. PSPDL was an α-pyran polysaccharide containing (1→4)-α-D-Glcp linkage. PSPDL inhibited the release of inflammatory factors and related gene mRNA expression, ameliorated oxidative stress, down-regulated the protein expression levels of p38, p-p38, IκB-α, p65, and p-p65. Conclusion: PSPDL exhibited anti-inflammatory activity, perhaps by regulating inflammatory mRNA expression and modulating the mitogen-activated protein kinases/nuclear factor kappa B (MAPK/NF-κB) signaling pathway. This finding provides a scientific basis for the development and utilization of PSPDL resources. |
| format | Article |
| id | doaj-art-67ae35e7ef1d464e84fdc16390b8e2cf |
| institution | Kabale University |
| issn | 1002-6630 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-67ae35e7ef1d464e84fdc16390b8e2cf2025-02-05T09:08:01ZengChina Food Publishing CompanyShipin Kexue1002-66302024-12-01452412813810.7506/spkx1002-6630-20240417-166Structural Characterization of Polygonum divaricatum L. Polysaccharide and Its Inhibitory Effect on Lipopolysaccharide-Induced Inflammation in RAW264.7 CellsCUI Yanyan, YUAN Yongxu, GUO Mingkun, HE Wenbing, LI Ming, PEI Shichun, LI Dajun0(1. Changbai Mountain Edible Plant Resources Development Engineering Center, Antibody Development Jilin Province University-Enterprise Joint Technology Innovation Labs, Food Science and Engineering, Tonghua Normal University, Tonghua 134002, China; 2. Jilin Shuangzheng Diagnostic Monoclonal Antibody Scientist Studio, Jilin Surge Medical Technology Co. Ltd., Tonghua 134001, China; 3. School of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China)Objective: To explore the in vitro anti-inflammatory activity and action mechanism of a polysaccharide extracted from Polygonum divaricatum L. (PSPDL). Methods: The structure of PSPDL was characterized using high-performance gel permeation chromatography (HPGPC), high-performance liquid chromatography (HPLC), Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and nuclear magnetic resonance spectroscopy (NMR). The in vitro anti-inflammatory activity and action mechanism of PSPDL were investigated in lipopolysaccharide (LPS)-induced RAW264.7 cells. Results: PSPDL had a relative molecular mass of 59.475 kDa and consisted mainly of mannose (Man), rhamnose (Rha), glucuronic acid (GlcA), galacturonic acid (GalA), glucose (Glc), galactose (Gal), and arabinose (Ara), with a molar ratio of 1.69:4.95:1.04:21.79:19.01:31.68:19.84. PSPDL was an α-pyran polysaccharide containing (1→4)-α-D-Glcp linkage. PSPDL inhibited the release of inflammatory factors and related gene mRNA expression, ameliorated oxidative stress, down-regulated the protein expression levels of p38, p-p38, IκB-α, p65, and p-p65. Conclusion: PSPDL exhibited anti-inflammatory activity, perhaps by regulating inflammatory mRNA expression and modulating the mitogen-activated protein kinases/nuclear factor kappa B (MAPK/NF-κB) signaling pathway. This finding provides a scientific basis for the development and utilization of PSPDL resources.https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-24-015.pdfpolygonum divaricatum l. polysaccharide; lipopolysaccharide; raw264.7 cells; inflammation; mitogen-activated protein kinases/nuclear factor kappa b signaling pathway |
| spellingShingle | CUI Yanyan, YUAN Yongxu, GUO Mingkun, HE Wenbing, LI Ming, PEI Shichun, LI Dajun Structural Characterization of Polygonum divaricatum L. Polysaccharide and Its Inhibitory Effect on Lipopolysaccharide-Induced Inflammation in RAW264.7 Cells Shipin Kexue polygonum divaricatum l. polysaccharide; lipopolysaccharide; raw264.7 cells; inflammation; mitogen-activated protein kinases/nuclear factor kappa b signaling pathway |
| title | Structural Characterization of Polygonum divaricatum L. Polysaccharide and Its Inhibitory Effect on Lipopolysaccharide-Induced Inflammation in RAW264.7 Cells |
| title_full | Structural Characterization of Polygonum divaricatum L. Polysaccharide and Its Inhibitory Effect on Lipopolysaccharide-Induced Inflammation in RAW264.7 Cells |
| title_fullStr | Structural Characterization of Polygonum divaricatum L. Polysaccharide and Its Inhibitory Effect on Lipopolysaccharide-Induced Inflammation in RAW264.7 Cells |
| title_full_unstemmed | Structural Characterization of Polygonum divaricatum L. Polysaccharide and Its Inhibitory Effect on Lipopolysaccharide-Induced Inflammation in RAW264.7 Cells |
| title_short | Structural Characterization of Polygonum divaricatum L. Polysaccharide and Its Inhibitory Effect on Lipopolysaccharide-Induced Inflammation in RAW264.7 Cells |
| title_sort | structural characterization of polygonum divaricatum l polysaccharide and its inhibitory effect on lipopolysaccharide induced inflammation in raw264 7 cells |
| topic | polygonum divaricatum l. polysaccharide; lipopolysaccharide; raw264.7 cells; inflammation; mitogen-activated protein kinases/nuclear factor kappa b signaling pathway |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-24-015.pdf |
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