Protein Detection with Potentiometric Aptasensors: A Comparative Study between Polyaniline and Single-Walled Carbon Nanotubes Transducers
A comparison study on the performance characteristics and surface characterization of two different solid-contact selective potentiometric thrombin aptasensors, one exploiting a network of single-walled carbon nanotubes (SWCNTs) and the other the polyaniline (PANI), both acting as a transducing elem...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/282756 |
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| author | Ali Düzgün Hassan Imran Kalle Levon F. Xavier Rius |
| author_facet | Ali Düzgün Hassan Imran Kalle Levon F. Xavier Rius |
| author_sort | Ali Düzgün |
| collection | DOAJ |
| description | A comparison study on the performance characteristics and surface characterization of two different solid-contact selective potentiometric thrombin aptasensors, one exploiting a network of single-walled carbon nanotubes (SWCNTs) and the other the polyaniline (PANI), both acting as a transducing element, is described in this work. The molecular properties of both SWCNT and PANI surfaces have been modified by covalently linking thrombin binding aptamers as biorecognition elements. The two aptasensors are compared and characterized through potentiometry and electrochemical impedance spectroscopy (EIS) based on the voltammetric response of multiply charged transition metal cations (such as hexaammineruthenium, [Ru(NH3)6]3+) bound electrostatically to the DNA probes. The surface densities of aptamers were accurately determined by the integration of the peak for the reduction of [Ru(NH3)6]3+ to [Ru(NH3)6]2+. The differences and the similarities, as well as the transduction mechanism, are also discussed. The sensitivity is calculated as 2.97 mV/decade and 8.03 mV/decade for the PANI and SWCNTs aptasensors, respectively. These results are in accordance with the higher surface density of the aptamers in the SWCNT potentiometric sensor. |
| format | Article |
| id | doaj-art-67a00ebf4f4040d996ededdf4310557d |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-67a00ebf4f4040d996ededdf4310557d2025-02-03T00:59:05ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/282756282756Protein Detection with Potentiometric Aptasensors: A Comparative Study between Polyaniline and Single-Walled Carbon Nanotubes TransducersAli Düzgün0Hassan Imran1Kalle Levon2F. Xavier Rius3Department of Analytical and Organic Chemistry, Universitat Rovira i Virgili, Campus Sescelades, Marcel·lí Domingo, 43007 Tarragona, SpainDepartment of Chemical and Biological Sciences, Polytechnic Institute of New York University, Brooklyn, NY 11201, USADepartment of Chemical and Biological Sciences, Polytechnic Institute of New York University, Brooklyn, NY 11201, USADepartment of Analytical and Organic Chemistry, Universitat Rovira i Virgili, Campus Sescelades, Marcel·lí Domingo, 43007 Tarragona, SpainA comparison study on the performance characteristics and surface characterization of two different solid-contact selective potentiometric thrombin aptasensors, one exploiting a network of single-walled carbon nanotubes (SWCNTs) and the other the polyaniline (PANI), both acting as a transducing element, is described in this work. The molecular properties of both SWCNT and PANI surfaces have been modified by covalently linking thrombin binding aptamers as biorecognition elements. The two aptasensors are compared and characterized through potentiometry and electrochemical impedance spectroscopy (EIS) based on the voltammetric response of multiply charged transition metal cations (such as hexaammineruthenium, [Ru(NH3)6]3+) bound electrostatically to the DNA probes. The surface densities of aptamers were accurately determined by the integration of the peak for the reduction of [Ru(NH3)6]3+ to [Ru(NH3)6]2+. The differences and the similarities, as well as the transduction mechanism, are also discussed. The sensitivity is calculated as 2.97 mV/decade and 8.03 mV/decade for the PANI and SWCNTs aptasensors, respectively. These results are in accordance with the higher surface density of the aptamers in the SWCNT potentiometric sensor.http://dx.doi.org/10.1155/2013/282756 |
| spellingShingle | Ali Düzgün Hassan Imran Kalle Levon F. Xavier Rius Protein Detection with Potentiometric Aptasensors: A Comparative Study between Polyaniline and Single-Walled Carbon Nanotubes Transducers The Scientific World Journal |
| title | Protein Detection with Potentiometric Aptasensors: A Comparative Study between Polyaniline and Single-Walled Carbon Nanotubes Transducers |
| title_full | Protein Detection with Potentiometric Aptasensors: A Comparative Study between Polyaniline and Single-Walled Carbon Nanotubes Transducers |
| title_fullStr | Protein Detection with Potentiometric Aptasensors: A Comparative Study between Polyaniline and Single-Walled Carbon Nanotubes Transducers |
| title_full_unstemmed | Protein Detection with Potentiometric Aptasensors: A Comparative Study between Polyaniline and Single-Walled Carbon Nanotubes Transducers |
| title_short | Protein Detection with Potentiometric Aptasensors: A Comparative Study between Polyaniline and Single-Walled Carbon Nanotubes Transducers |
| title_sort | protein detection with potentiometric aptasensors a comparative study between polyaniline and single walled carbon nanotubes transducers |
| url | http://dx.doi.org/10.1155/2013/282756 |
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