Determination of Polyphenol Components of Korean Prostrate Spurge (Euphorbia supina) by Using Liquid Chromatography—Tandem Mass Spectrometry: Overall Contribution to Antioxidant Activity
The Korean prostrate spurge Euphorbia supina is a weed that has been used in folk medicine in Korea against a variety of diseases. Nine polyphenols were characterized for this plant by using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and the results were compared wi...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2014/418690 |
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| author | Yi Song Sung Woo Jeong Won Sup Lee Semin Park Yun-Hi Kim Gon-Sup Kim Soo Jung Lee Jong Sung Jin Chi-Yeon Kim Ji Eun Lee Se Yun Ok Ki-Min Bark Sung Chul Shin |
| author_facet | Yi Song Sung Woo Jeong Won Sup Lee Semin Park Yun-Hi Kim Gon-Sup Kim Soo Jung Lee Jong Sung Jin Chi-Yeon Kim Ji Eun Lee Se Yun Ok Ki-Min Bark Sung Chul Shin |
| author_sort | Yi Song |
| collection | DOAJ |
| description | The Korean prostrate spurge Euphorbia supina is a weed that has been used in folk medicine in Korea against a variety of diseases. Nine polyphenols were characterized for this plant by using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and the results were compared with the literature data. The individual components were validated using the calibration curves of structurally related external standards and quantified for the first time by using the validated method. Correlation coefficients (r2) were >0.9907. The limit of detection and limit of quantification of the method were >0.028 mg/L and 0.094 mg/L, respectively. Recoveries measured at 50 mg/L and 100 mg/L were 76.1–102.8% and 85.2–98.6%, respectively. The total amount of the identified polyphenols was 3352.9 ± 2.8 mg/kg fresh plant. Quercetin and kaempferol derivatives formed 84.8% of the total polyphenols. The antioxidant activities of the flavonoids were evaluated in terms of 1,1-diphenyl-2-picrylhydrazyl and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation-scavenging activity, and the reducing power showed a dose-dependent increase. Cell viability was effectively suppressed at polyphenol mixture concentrations >250 mg/L. |
| format | Article |
| id | doaj-art-67750a3af879492286cf8973e89d257f |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-67750a3af879492286cf8973e89d257f2025-02-03T01:27:33ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732014-01-01201410.1155/2014/418690418690Determination of Polyphenol Components of Korean Prostrate Spurge (Euphorbia supina) by Using Liquid Chromatography—Tandem Mass Spectrometry: Overall Contribution to Antioxidant ActivityYi Song0Sung Woo Jeong1Won Sup Lee2Semin Park3Yun-Hi Kim4Gon-Sup Kim5Soo Jung Lee6Jong Sung Jin7Chi-Yeon Kim8Ji Eun Lee9Se Yun Ok10Ki-Min Bark11Sung Chul Shin12Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of KoreaDepartment of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of KoreaDepartment of Internal Medicine, Institute of Health Sciences and Gyeongnam Regional Cancer Center, Gyeongsang National University, Jinju 660-702, Republic of KoreaDepartment of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of KoreaDepartment of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of KoreaResearch Institute of Life Science and College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of KoreaDepartment of Food and Nutrition, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of KoreaDivision of High Technology Materials Research, Busan Center, Korea Basic Science Institute (KBSI), Busan 618-230, Republic of KoreaDepartment of Dermatology Institute of Health Science, Gyeongsang National University Hospital, Jinju 660-702, Republic of KoreaDepartment of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of KoreaDepartment of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of KoreaDepartment of Chemical Education and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of KoreaDepartment of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of KoreaThe Korean prostrate spurge Euphorbia supina is a weed that has been used in folk medicine in Korea against a variety of diseases. Nine polyphenols were characterized for this plant by using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and the results were compared with the literature data. The individual components were validated using the calibration curves of structurally related external standards and quantified for the first time by using the validated method. Correlation coefficients (r2) were >0.9907. The limit of detection and limit of quantification of the method were >0.028 mg/L and 0.094 mg/L, respectively. Recoveries measured at 50 mg/L and 100 mg/L were 76.1–102.8% and 85.2–98.6%, respectively. The total amount of the identified polyphenols was 3352.9 ± 2.8 mg/kg fresh plant. Quercetin and kaempferol derivatives formed 84.8% of the total polyphenols. The antioxidant activities of the flavonoids were evaluated in terms of 1,1-diphenyl-2-picrylhydrazyl and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation-scavenging activity, and the reducing power showed a dose-dependent increase. Cell viability was effectively suppressed at polyphenol mixture concentrations >250 mg/L.http://dx.doi.org/10.1155/2014/418690 |
| spellingShingle | Yi Song Sung Woo Jeong Won Sup Lee Semin Park Yun-Hi Kim Gon-Sup Kim Soo Jung Lee Jong Sung Jin Chi-Yeon Kim Ji Eun Lee Se Yun Ok Ki-Min Bark Sung Chul Shin Determination of Polyphenol Components of Korean Prostrate Spurge (Euphorbia supina) by Using Liquid Chromatography—Tandem Mass Spectrometry: Overall Contribution to Antioxidant Activity Journal of Analytical Methods in Chemistry |
| title | Determination of Polyphenol Components of Korean Prostrate Spurge (Euphorbia supina) by Using Liquid Chromatography—Tandem Mass Spectrometry: Overall Contribution to Antioxidant Activity |
| title_full | Determination of Polyphenol Components of Korean Prostrate Spurge (Euphorbia supina) by Using Liquid Chromatography—Tandem Mass Spectrometry: Overall Contribution to Antioxidant Activity |
| title_fullStr | Determination of Polyphenol Components of Korean Prostrate Spurge (Euphorbia supina) by Using Liquid Chromatography—Tandem Mass Spectrometry: Overall Contribution to Antioxidant Activity |
| title_full_unstemmed | Determination of Polyphenol Components of Korean Prostrate Spurge (Euphorbia supina) by Using Liquid Chromatography—Tandem Mass Spectrometry: Overall Contribution to Antioxidant Activity |
| title_short | Determination of Polyphenol Components of Korean Prostrate Spurge (Euphorbia supina) by Using Liquid Chromatography—Tandem Mass Spectrometry: Overall Contribution to Antioxidant Activity |
| title_sort | determination of polyphenol components of korean prostrate spurge euphorbia supina by using liquid chromatography tandem mass spectrometry overall contribution to antioxidant activity |
| url | http://dx.doi.org/10.1155/2014/418690 |
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