Genome-wide investigation of ABCB, PIN, and AUX/LAX gene families and their involvement in the formation of leaf protrusions in Sesamum indicum
Sesamum indicum, a highly esteemed oil crop, has exhibited remarkable value and potential in diverse areas encompassing the economy, food industry, and health. We have observed that there are small protrusions on the leaves of the indehiscent capsule material G1358. No obvious difference was detecte...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1526321/full |
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| author | Yanli Li Yujia Ma Huihui Gong Xinxiao Cui Xin Wang Yuling Dong Ying Chen Junsheng Zhao |
| author_facet | Yanli Li Yujia Ma Huihui Gong Xinxiao Cui Xin Wang Yuling Dong Ying Chen Junsheng Zhao |
| author_sort | Yanli Li |
| collection | DOAJ |
| description | Sesamum indicum, a highly esteemed oil crop, has exhibited remarkable value and potential in diverse areas encompassing the economy, food industry, and health. We have observed that there are small protrusions on the leaves of the indehiscent capsule material G1358. No obvious difference was detected on overall auxin content between the leaves of G1358 and LZ1 from metabolomic analysis. However, auxin levels at the base of G1358 leaves were notably higher than in LZ1, suggesting a correlation between the small protrusions at the base and polar auxin transport (PAT). PAT is essential for regulating growth and development across different plant tissues. PAT primarily relies on three families of transporter proteins: ABCB, PIN, and AUX/LAX. However, the ABCB, PIN, and AUX/LAX protein families in Sesamum indicum have not been systematically characterized. Herein, we identified 21 SiABCBs, 11 SiPINs, and 5 SiLAXs in S. indicum. Our analysis indicated that tandem duplications have facilitated the expansion of SiLAX, SiPIN, and SiABCB gene families, which have undergone purifying selection throughout their evolutionary history. Transcriptome screening and RT-qPCR analysis revealed that SiABCB3, SiABCB6, and SiPIN10 positively regulate PAT, whereas SiABCB7 and SiABCB9 negatively regulate PAT in G1358. These regulatory interactions contribute to the formation of small protrusions in G1358 leaves and enhance the rate of photosynthesis. Our findings provide a theoretical foundation for understanding PAT genes and their roles in the environmental adaptation of sesame. |
| format | Article |
| id | doaj-art-676a1b096e674561a383dfbc83c7ed31 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-676a1b096e674561a383dfbc83c7ed312025-01-31T06:39:57ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.15263211526321Genome-wide investigation of ABCB, PIN, and AUX/LAX gene families and their involvement in the formation of leaf protrusions in Sesamum indicumYanli Li0Yujia Ma1Huihui Gong2Xinxiao Cui3Xin Wang4Yuling Dong5Ying Chen6Junsheng Zhao7Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan, Shandong, ChinaCollege of Life Sciences, Shandong Normal University, Jinan, Shandong, ChinaInstitute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan, Shandong, ChinaInstitute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan, Shandong, ChinaInstitute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan, Shandong, ChinaInstitute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan, Shandong, ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong, ChinaInstitute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan, Shandong, ChinaSesamum indicum, a highly esteemed oil crop, has exhibited remarkable value and potential in diverse areas encompassing the economy, food industry, and health. We have observed that there are small protrusions on the leaves of the indehiscent capsule material G1358. No obvious difference was detected on overall auxin content between the leaves of G1358 and LZ1 from metabolomic analysis. However, auxin levels at the base of G1358 leaves were notably higher than in LZ1, suggesting a correlation between the small protrusions at the base and polar auxin transport (PAT). PAT is essential for regulating growth and development across different plant tissues. PAT primarily relies on three families of transporter proteins: ABCB, PIN, and AUX/LAX. However, the ABCB, PIN, and AUX/LAX protein families in Sesamum indicum have not been systematically characterized. Herein, we identified 21 SiABCBs, 11 SiPINs, and 5 SiLAXs in S. indicum. Our analysis indicated that tandem duplications have facilitated the expansion of SiLAX, SiPIN, and SiABCB gene families, which have undergone purifying selection throughout their evolutionary history. Transcriptome screening and RT-qPCR analysis revealed that SiABCB3, SiABCB6, and SiPIN10 positively regulate PAT, whereas SiABCB7 and SiABCB9 negatively regulate PAT in G1358. These regulatory interactions contribute to the formation of small protrusions in G1358 leaves and enhance the rate of photosynthesis. Our findings provide a theoretical foundation for understanding PAT genes and their roles in the environmental adaptation of sesame.https://www.frontiersin.org/articles/10.3389/fpls.2024.1526321/fullPATABCBPINLAXSesamum indicumleaf protrusion |
| spellingShingle | Yanli Li Yujia Ma Huihui Gong Xinxiao Cui Xin Wang Yuling Dong Ying Chen Junsheng Zhao Genome-wide investigation of ABCB, PIN, and AUX/LAX gene families and their involvement in the formation of leaf protrusions in Sesamum indicum Frontiers in Plant Science PAT ABCB PIN LAX Sesamum indicum leaf protrusion |
| title | Genome-wide investigation of ABCB, PIN, and AUX/LAX gene families and their involvement in the formation of leaf protrusions in Sesamum indicum |
| title_full | Genome-wide investigation of ABCB, PIN, and AUX/LAX gene families and their involvement in the formation of leaf protrusions in Sesamum indicum |
| title_fullStr | Genome-wide investigation of ABCB, PIN, and AUX/LAX gene families and their involvement in the formation of leaf protrusions in Sesamum indicum |
| title_full_unstemmed | Genome-wide investigation of ABCB, PIN, and AUX/LAX gene families and their involvement in the formation of leaf protrusions in Sesamum indicum |
| title_short | Genome-wide investigation of ABCB, PIN, and AUX/LAX gene families and their involvement in the formation of leaf protrusions in Sesamum indicum |
| title_sort | genome wide investigation of abcb pin and aux lax gene families and their involvement in the formation of leaf protrusions in sesamum indicum |
| topic | PAT ABCB PIN LAX Sesamum indicum leaf protrusion |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1526321/full |
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