THE EFFECT OF DELAY IN TEMPERATURE VARIATIONS ON RESULTS OF GLUCOSE TEST IN DM PATIENTS WITH HYPERCHOLESTEROLEMIA

Background: The pre-analytic stage has the most significant contribution to error, which is 60% to 70%. This is an excellent contribution to the dependability of laboratory results. Delays in the examination often occur in the laboratory due to various factors influencing glucose examination outcome...

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Main Authors: Chori Khotul Ula, Edy Haryanto Edy Haryanto, Syamsul Arifin Syamsul Arifin, Wisnu Istanto
Format: Article
Language:English
Published: Universitas Airlangga 2023-07-01
Series:Journal of Vocational Health Studies
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Online Access:https://e-journal.unair.ac.id/JVHS/article/view/38168
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author Chori Khotul Ula
Edy Haryanto Edy Haryanto
Syamsul Arifin Syamsul Arifin
Wisnu Istanto
author_facet Chori Khotul Ula
Edy Haryanto Edy Haryanto
Syamsul Arifin Syamsul Arifin
Wisnu Istanto
author_sort Chori Khotul Ula
collection DOAJ
description Background: The pre-analytic stage has the most significant contribution to error, which is 60% to 70%. This is an excellent contribution to the dependability of laboratory results. Delays in the examination often occur in the laboratory due to various factors influencing glucose examination outcomes. Purpose: To ascertain the effect of long delays in various temperatures on the results of plasma NaF and serum glucose tests in diabetes mellitus patients who have hypercholesterolemia. Method: Pre-test and post-test design used in a pre-experimental method. In the pretest, plasma NaF and serum glucose levels were checked immediately. In the posttest, the plasma NaF and serum samples were delayed for 4 and 8 hours at the temperature of the refrigerator (4°C) and room temperature (25°C). Result: The results of One-way ANOVA test analysis of the plasma NaF glucose level with an immediate examination, a delay of 4 and 8 hours at room temperature (25°C) showed no effect with a Sig. of 0.423 > 0.05. Plasma NaF glucose level with an immediate examination, 4 and 8 hours delay in refrigerator temperature (4°C) showed no effect with Sig. of 0.772 > 0.05. Serum glucose levels with an immediate examination, 4 and 8 hours delay at room temperature (25°C) showed no effect with a Sig. of 0.333 > 0.05. Serum glucose levels with an immediate examination, 4 and 8 hours delay in refrigerator temperature (4°C) showed no effect with a Sig. of 0.604 > 0.05. Conclusion: There was no effect of 4 hours and 8 hours of delay at the temperature of the refrigerator (4°C) and room temperature (25°C) on the results of NaF and serum glucose examinations in patients with diabetes mellitus who have hypercholesterolemia.
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publishDate 2023-07-01
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spelling doaj-art-674b618c471d480cacb04b813c2d27e72025-02-02T17:44:06ZengUniversitas AirlanggaJournal of Vocational Health Studies2580-71612580-717X2023-07-0171101610.20473/jvhs.V7.I1.2023.10-1636237THE EFFECT OF DELAY IN TEMPERATURE VARIATIONS ON RESULTS OF GLUCOSE TEST IN DM PATIENTS WITH HYPERCHOLESTEROLEMIAChori Khotul Ula0https://orcid.org/0009-0005-3024-2531Edy Haryanto Edy Haryanto1https://orcid.org/0009-0002-8269-9283Syamsul Arifin Syamsul Arifin2https://orcid.org/0000-0002-0319-2605Wisnu Istanto3https://orcid.org/0000-0001-8392-0272Department of Medical Laboratory Technology, Health Polytechnic Ministry of Health Surabaya, IndonesiaDepartment of Medical Laboratory Technology, Health Polytechnic Ministry of Health Surabaya, IndonesiaDepartment of Medical Laboratory Technology, Health Polytechnic Ministry of Health Surabaya, IndonesiaDepartment of Medical Laboratory Technology, Health Polytechnic Ministry of Health Surabaya, IndonesiaBackground: The pre-analytic stage has the most significant contribution to error, which is 60% to 70%. This is an excellent contribution to the dependability of laboratory results. Delays in the examination often occur in the laboratory due to various factors influencing glucose examination outcomes. Purpose: To ascertain the effect of long delays in various temperatures on the results of plasma NaF and serum glucose tests in diabetes mellitus patients who have hypercholesterolemia. Method: Pre-test and post-test design used in a pre-experimental method. In the pretest, plasma NaF and serum glucose levels were checked immediately. In the posttest, the plasma NaF and serum samples were delayed for 4 and 8 hours at the temperature of the refrigerator (4°C) and room temperature (25°C). Result: The results of One-way ANOVA test analysis of the plasma NaF glucose level with an immediate examination, a delay of 4 and 8 hours at room temperature (25°C) showed no effect with a Sig. of 0.423 > 0.05. Plasma NaF glucose level with an immediate examination, 4 and 8 hours delay in refrigerator temperature (4°C) showed no effect with Sig. of 0.772 > 0.05. Serum glucose levels with an immediate examination, 4 and 8 hours delay at room temperature (25°C) showed no effect with a Sig. of 0.333 > 0.05. Serum glucose levels with an immediate examination, 4 and 8 hours delay in refrigerator temperature (4°C) showed no effect with a Sig. of 0.604 > 0.05. Conclusion: There was no effect of 4 hours and 8 hours of delay at the temperature of the refrigerator (4°C) and room temperature (25°C) on the results of NaF and serum glucose examinations in patients with diabetes mellitus who have hypercholesterolemia.https://e-journal.unair.ac.id/JVHS/article/view/38168glucoseplasma nafserum delayed timedelayed temperature
spellingShingle Chori Khotul Ula
Edy Haryanto Edy Haryanto
Syamsul Arifin Syamsul Arifin
Wisnu Istanto
THE EFFECT OF DELAY IN TEMPERATURE VARIATIONS ON RESULTS OF GLUCOSE TEST IN DM PATIENTS WITH HYPERCHOLESTEROLEMIA
Journal of Vocational Health Studies
glucose
plasma naf
serum
delayed time
delayed temperature
title THE EFFECT OF DELAY IN TEMPERATURE VARIATIONS ON RESULTS OF GLUCOSE TEST IN DM PATIENTS WITH HYPERCHOLESTEROLEMIA
title_full THE EFFECT OF DELAY IN TEMPERATURE VARIATIONS ON RESULTS OF GLUCOSE TEST IN DM PATIENTS WITH HYPERCHOLESTEROLEMIA
title_fullStr THE EFFECT OF DELAY IN TEMPERATURE VARIATIONS ON RESULTS OF GLUCOSE TEST IN DM PATIENTS WITH HYPERCHOLESTEROLEMIA
title_full_unstemmed THE EFFECT OF DELAY IN TEMPERATURE VARIATIONS ON RESULTS OF GLUCOSE TEST IN DM PATIENTS WITH HYPERCHOLESTEROLEMIA
title_short THE EFFECT OF DELAY IN TEMPERATURE VARIATIONS ON RESULTS OF GLUCOSE TEST IN DM PATIENTS WITH HYPERCHOLESTEROLEMIA
title_sort effect of delay in temperature variations on results of glucose test in dm patients with hypercholesterolemia
topic glucose
plasma naf
serum
delayed time
delayed temperature
url https://e-journal.unair.ac.id/JVHS/article/view/38168
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