3D nanocomposites of β-TCP-H3BO3-Cu with improved mechanical and biological performances for bone regeneration applications
Abstract Recently, 3-D porous architecture of the composites play a key role in cell proliferation, bone regeneration, and anticancer activities. The osteoinductive and osteoconductive properties of β-TCP allow for the complete repair of numerous bone defects. Herein, β-TCP was synthesized by wet ch...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-87988-4 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832585711895707648 |
|---|---|
| author | Sarvesh Kumar Avinashi Rajat Kumar Mishra Shweta Saurabh Kumar Amreen Shamsad Shama Parveen Surajita Sahu Savita Kumari Zaireen Fatima Sachin Kumar Yadav Monisha Banerjee Monalisa Mishra Neeraj Mehta Chandki Ram Gautam |
| author_facet | Sarvesh Kumar Avinashi Rajat Kumar Mishra Shweta Saurabh Kumar Amreen Shamsad Shama Parveen Surajita Sahu Savita Kumari Zaireen Fatima Sachin Kumar Yadav Monisha Banerjee Monalisa Mishra Neeraj Mehta Chandki Ram Gautam |
| author_sort | Sarvesh Kumar Avinashi |
| collection | DOAJ |
| description | Abstract Recently, 3-D porous architecture of the composites play a key role in cell proliferation, bone regeneration, and anticancer activities. The osteoinductive and osteoconductive properties of β-TCP allow for the complete repair of numerous bone defects. Herein, β-TCP was synthesized by wet chemical precipitation route, and their 3-D porous composites with H3BO3 and Cu nanoparticles were prepared by the solid-state reaction method with improved mechanical and biological performances. Several characterization techniques have been used to investigate the various characteristics of fabricated porous composites. SEM and TEM studies revealed the porous morphology and hexagonal sheets of the β-TCP for the composite THC8 (82TCP-10H3BO3-8Cu). Moreover, the mechanical study showed excellent compressive strength (188 MPa), a high Young’s modulus (2.84 GPa), and elevated fracture toughness (9.11 MPa.m1/2). An in vitro study by MTT assay on osteoblast (MG-63) cells demonstrated no or minimal cytotoxicity at the higher concentration, 100 µg/ml after 24 h and it was found a more pronounced result at 20 µg/ml on increasing the concentration of Cu nanoparticles after incubating 72 h. The THC12 composite showed the highest antibacterial potency exclusively against B. subtilis. S. pyogene, S. typhi and E. coli. at 10 mg/ml, indicating its potential effectiveness in inhibiting all of these pathogens. Genotoxicity and cytotoxicity tests were also performed on rearing Drosophila melanogaster, and these findings did not detect any trypan blue-positive staining, which further recommended that the existence of composites did not harm the larval gut. Therefore, the fabricated porous composites THC8 and THC12 are suitable for bone regrowth without harming the surrounding cells and protect against bacterial infections. |
| format | Article |
| id | doaj-art-6745c6c08e164a379c29db591f22d820 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-6745c6c08e164a379c29db591f22d8202025-01-26T12:34:23ZengNature PortfolioScientific Reports2045-23222025-01-0115112510.1038/s41598-025-87988-43D nanocomposites of β-TCP-H3BO3-Cu with improved mechanical and biological performances for bone regeneration applicationsSarvesh Kumar Avinashi0Rajat Kumar Mishra1Shweta2Saurabh Kumar3Amreen Shamsad4Shama Parveen5Surajita Sahu6Savita Kumari7Zaireen Fatima8Sachin Kumar Yadav9Monisha Banerjee10Monalisa Mishra11Neeraj Mehta12Chandki Ram Gautam13Advanced Glass and Glass Ceramic Research Laboratory, Department of Physics, University of LucknowAdvanced Glass and Glass Ceramic Research Laboratory, Department of Physics, University of LucknowAdvanced Glass and Glass Ceramic Research Laboratory, Department of Physics, University of LucknowMolecular and Human Genetics Laboratory, Department of Zoology, University of LucknowMolecular and Human Genetics Laboratory, Department of Zoology, University of LucknowMolecular and Human Genetics Laboratory, Department of Zoology, University of LucknowNeural Developmental Biology Lab, Department of Life Science, NIT RourkelaAdvanced Glass and Glass Ceramic Research Laboratory, Department of Physics, University of LucknowAdvanced Glass and Glass Ceramic Research Laboratory, Department of Physics, University of LucknowDepartment of Physics, Banaras Hindu UniversityMolecular and Human Genetics Laboratory, Department of Zoology, University of LucknowNeural Developmental Biology Lab, Department of Life Science, NIT RourkelaDepartment of Physics, Banaras Hindu UniversityAdvanced Glass and Glass Ceramic Research Laboratory, Department of Physics, University of LucknowAbstract Recently, 3-D porous architecture of the composites play a key role in cell proliferation, bone regeneration, and anticancer activities. The osteoinductive and osteoconductive properties of β-TCP allow for the complete repair of numerous bone defects. Herein, β-TCP was synthesized by wet chemical precipitation route, and their 3-D porous composites with H3BO3 and Cu nanoparticles were prepared by the solid-state reaction method with improved mechanical and biological performances. Several characterization techniques have been used to investigate the various characteristics of fabricated porous composites. SEM and TEM studies revealed the porous morphology and hexagonal sheets of the β-TCP for the composite THC8 (82TCP-10H3BO3-8Cu). Moreover, the mechanical study showed excellent compressive strength (188 MPa), a high Young’s modulus (2.84 GPa), and elevated fracture toughness (9.11 MPa.m1/2). An in vitro study by MTT assay on osteoblast (MG-63) cells demonstrated no or minimal cytotoxicity at the higher concentration, 100 µg/ml after 24 h and it was found a more pronounced result at 20 µg/ml on increasing the concentration of Cu nanoparticles after incubating 72 h. The THC12 composite showed the highest antibacterial potency exclusively against B. subtilis. S. pyogene, S. typhi and E. coli. at 10 mg/ml, indicating its potential effectiveness in inhibiting all of these pathogens. Genotoxicity and cytotoxicity tests were also performed on rearing Drosophila melanogaster, and these findings did not detect any trypan blue-positive staining, which further recommended that the existence of composites did not harm the larval gut. Therefore, the fabricated porous composites THC8 and THC12 are suitable for bone regrowth without harming the surrounding cells and protect against bacterial infections.https://doi.org/10.1038/s41598-025-87988-4β-tricalcium phosphateCompositesMorphologyMechanical performancesIn vitro-studyBone repair |
| spellingShingle | Sarvesh Kumar Avinashi Rajat Kumar Mishra Shweta Saurabh Kumar Amreen Shamsad Shama Parveen Surajita Sahu Savita Kumari Zaireen Fatima Sachin Kumar Yadav Monisha Banerjee Monalisa Mishra Neeraj Mehta Chandki Ram Gautam 3D nanocomposites of β-TCP-H3BO3-Cu with improved mechanical and biological performances for bone regeneration applications Scientific Reports β-tricalcium phosphate Composites Morphology Mechanical performances In vitro-study Bone repair |
| title | 3D nanocomposites of β-TCP-H3BO3-Cu with improved mechanical and biological performances for bone regeneration applications |
| title_full | 3D nanocomposites of β-TCP-H3BO3-Cu with improved mechanical and biological performances for bone regeneration applications |
| title_fullStr | 3D nanocomposites of β-TCP-H3BO3-Cu with improved mechanical and biological performances for bone regeneration applications |
| title_full_unstemmed | 3D nanocomposites of β-TCP-H3BO3-Cu with improved mechanical and biological performances for bone regeneration applications |
| title_short | 3D nanocomposites of β-TCP-H3BO3-Cu with improved mechanical and biological performances for bone regeneration applications |
| title_sort | 3d nanocomposites of β tcp h3bo3 cu with improved mechanical and biological performances for bone regeneration applications |
| topic | β-tricalcium phosphate Composites Morphology Mechanical performances In vitro-study Bone repair |
| url | https://doi.org/10.1038/s41598-025-87988-4 |
| work_keys_str_mv | AT sarveshkumaravinashi 3dnanocompositesofbtcph3bo3cuwithimprovedmechanicalandbiologicalperformancesforboneregenerationapplications AT rajatkumarmishra 3dnanocompositesofbtcph3bo3cuwithimprovedmechanicalandbiologicalperformancesforboneregenerationapplications AT shweta 3dnanocompositesofbtcph3bo3cuwithimprovedmechanicalandbiologicalperformancesforboneregenerationapplications AT saurabhkumar 3dnanocompositesofbtcph3bo3cuwithimprovedmechanicalandbiologicalperformancesforboneregenerationapplications AT amreenshamsad 3dnanocompositesofbtcph3bo3cuwithimprovedmechanicalandbiologicalperformancesforboneregenerationapplications AT shamaparveen 3dnanocompositesofbtcph3bo3cuwithimprovedmechanicalandbiologicalperformancesforboneregenerationapplications AT surajitasahu 3dnanocompositesofbtcph3bo3cuwithimprovedmechanicalandbiologicalperformancesforboneregenerationapplications AT savitakumari 3dnanocompositesofbtcph3bo3cuwithimprovedmechanicalandbiologicalperformancesforboneregenerationapplications AT zaireenfatima 3dnanocompositesofbtcph3bo3cuwithimprovedmechanicalandbiologicalperformancesforboneregenerationapplications AT sachinkumaryadav 3dnanocompositesofbtcph3bo3cuwithimprovedmechanicalandbiologicalperformancesforboneregenerationapplications AT monishabanerjee 3dnanocompositesofbtcph3bo3cuwithimprovedmechanicalandbiologicalperformancesforboneregenerationapplications AT monalisamishra 3dnanocompositesofbtcph3bo3cuwithimprovedmechanicalandbiologicalperformancesforboneregenerationapplications AT neerajmehta 3dnanocompositesofbtcph3bo3cuwithimprovedmechanicalandbiologicalperformancesforboneregenerationapplications AT chandkiramgautam 3dnanocompositesofbtcph3bo3cuwithimprovedmechanicalandbiologicalperformancesforboneregenerationapplications |