Can fungal endophytes suppress Trialeurodes vaporariorum and the transmission of tomato infectious chlorosis and chlorosis viruses in field conditions?
Field trials were conducted for two seasons in two experimental sites (Mwea in Kirinyaga and Ngoliba in Kiambu counties of Kenya) to assess the efficacy of fungal endophytes Hypocrea lixii F3ST1 and Trichoderma asperellum M2RT4 in the control of Trialeurodes vaporariorum vector of tomato infectious...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-02-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1470821/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832542309602820096 |
|---|---|
| author | Marial Makur Zechariah Paweer Marial Makur Zechariah Paweer Everlyne Samita Namikoye Shem Bonuke Nchore Komivi Senyo Akutse Komivi Senyo Akutse |
| author_facet | Marial Makur Zechariah Paweer Marial Makur Zechariah Paweer Everlyne Samita Namikoye Shem Bonuke Nchore Komivi Senyo Akutse Komivi Senyo Akutse |
| author_sort | Marial Makur Zechariah Paweer |
| collection | DOAJ |
| description | Field trials were conducted for two seasons in two experimental sites (Mwea in Kirinyaga and Ngoliba in Kiambu counties of Kenya) to assess the efficacy of fungal endophytes Hypocrea lixii F3ST1 and Trichoderma asperellum M2RT4 in the control of Trialeurodes vaporariorum vector of tomato infectious chlorosis virus (TICV) and tomato chlorosis virus (ToCV) through seeds inoculation. TICV and ToCV’s disease incidence, severity and the yield were also evaluated. All the fungal endophytes successfully colonized all the tomato plant parts, but the highest root colonization was observed in H. lixii F3ST1 compared to the T. asperellum M2RT4 in both seasons. The number of nymphs was significantly lower in the endophytically colonized tomato plants than the control treatments in all the seasons and at both sites. However, the lowest number of nymphs was recorded in H. lixii F3ST1 compared to T. asperellum M2RT4. On the other hand, the TICV and ToCV disease incidence and severity rates were lower in endophytically colonized tomato crops compared to the control plots. This could be attributed to the reduction in the virus replication and lower feeding ability of T. vaporariorum that was characterized by less excretion of honeydew causing sooty mold. However, no significant difference was observed in ToCV disease severity rates among the treatments and across the seasons. The yield was significantly higher in endophyte plots than the control treatments in both sites and across the two seasons. This study demonstrates that H. lixii F3ST1 and T. asperellum M2RT4 endophytically colonized tomato plants and conferred systemic resistance against T. vaporariorum vector, and significantly reduced the transmission of TICV and ToCV, contributing to high reduction of both diseases’ incidence and severity in the field. However, further studies are warranted to confirm these results at large scale trials. |
| format | Article |
| id | doaj-art-67419703eee440c8bc81c7859c2dca6a |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-67419703eee440c8bc81c7859c2dca6a2025-02-04T06:31:43ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-02-011510.3389/fcimb.2025.14708211470821Can fungal endophytes suppress Trialeurodes vaporariorum and the transmission of tomato infectious chlorosis and chlorosis viruses in field conditions?Marial Makur Zechariah Paweer0Marial Makur Zechariah Paweer1Everlyne Samita Namikoye2Shem Bonuke Nchore3Komivi Senyo Akutse4Komivi Senyo Akutse5International Centre of Insect Physiology and Ecology (icipe), Nairobi, KenyaDepartment of Agricultural Science and Technology, Kenyatta University, Nairobi, KenyaDepartment of Agricultural Science and Technology, Kenyatta University, Nairobi, KenyaDepartment of Agricultural Science and Technology, Kenyatta University, Nairobi, KenyaInternational Centre of Insect Physiology and Ecology (icipe), Nairobi, KenyaUnit of Environmental Sciences and Management, North-West University, Potchefstroom, South AfricaField trials were conducted for two seasons in two experimental sites (Mwea in Kirinyaga and Ngoliba in Kiambu counties of Kenya) to assess the efficacy of fungal endophytes Hypocrea lixii F3ST1 and Trichoderma asperellum M2RT4 in the control of Trialeurodes vaporariorum vector of tomato infectious chlorosis virus (TICV) and tomato chlorosis virus (ToCV) through seeds inoculation. TICV and ToCV’s disease incidence, severity and the yield were also evaluated. All the fungal endophytes successfully colonized all the tomato plant parts, but the highest root colonization was observed in H. lixii F3ST1 compared to the T. asperellum M2RT4 in both seasons. The number of nymphs was significantly lower in the endophytically colonized tomato plants than the control treatments in all the seasons and at both sites. However, the lowest number of nymphs was recorded in H. lixii F3ST1 compared to T. asperellum M2RT4. On the other hand, the TICV and ToCV disease incidence and severity rates were lower in endophytically colonized tomato crops compared to the control plots. This could be attributed to the reduction in the virus replication and lower feeding ability of T. vaporariorum that was characterized by less excretion of honeydew causing sooty mold. However, no significant difference was observed in ToCV disease severity rates among the treatments and across the seasons. The yield was significantly higher in endophyte plots than the control treatments in both sites and across the two seasons. This study demonstrates that H. lixii F3ST1 and T. asperellum M2RT4 endophytically colonized tomato plants and conferred systemic resistance against T. vaporariorum vector, and significantly reduced the transmission of TICV and ToCV, contributing to high reduction of both diseases’ incidence and severity in the field. However, further studies are warranted to confirm these results at large scale trials.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1470821/fullTrichoderma asperellumHypocrea lixiiTrialeurodes vaporariorumtomato infectious chlorosis virusincidenceseverity |
| spellingShingle | Marial Makur Zechariah Paweer Marial Makur Zechariah Paweer Everlyne Samita Namikoye Shem Bonuke Nchore Komivi Senyo Akutse Komivi Senyo Akutse Can fungal endophytes suppress Trialeurodes vaporariorum and the transmission of tomato infectious chlorosis and chlorosis viruses in field conditions? Frontiers in Cellular and Infection Microbiology Trichoderma asperellum Hypocrea lixii Trialeurodes vaporariorum tomato infectious chlorosis virus incidence severity |
| title | Can fungal endophytes suppress Trialeurodes vaporariorum and the transmission of tomato infectious chlorosis and chlorosis viruses in field conditions? |
| title_full | Can fungal endophytes suppress Trialeurodes vaporariorum and the transmission of tomato infectious chlorosis and chlorosis viruses in field conditions? |
| title_fullStr | Can fungal endophytes suppress Trialeurodes vaporariorum and the transmission of tomato infectious chlorosis and chlorosis viruses in field conditions? |
| title_full_unstemmed | Can fungal endophytes suppress Trialeurodes vaporariorum and the transmission of tomato infectious chlorosis and chlorosis viruses in field conditions? |
| title_short | Can fungal endophytes suppress Trialeurodes vaporariorum and the transmission of tomato infectious chlorosis and chlorosis viruses in field conditions? |
| title_sort | can fungal endophytes suppress trialeurodes vaporariorum and the transmission of tomato infectious chlorosis and chlorosis viruses in field conditions |
| topic | Trichoderma asperellum Hypocrea lixii Trialeurodes vaporariorum tomato infectious chlorosis virus incidence severity |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1470821/full |
| work_keys_str_mv | AT marialmakurzechariahpaweer canfungalendophytessuppresstrialeurodesvaporariorumandthetransmissionoftomatoinfectiouschlorosisandchlorosisvirusesinfieldconditions AT marialmakurzechariahpaweer canfungalendophytessuppresstrialeurodesvaporariorumandthetransmissionoftomatoinfectiouschlorosisandchlorosisvirusesinfieldconditions AT everlynesamitanamikoye canfungalendophytessuppresstrialeurodesvaporariorumandthetransmissionoftomatoinfectiouschlorosisandchlorosisvirusesinfieldconditions AT shembonukenchore canfungalendophytessuppresstrialeurodesvaporariorumandthetransmissionoftomatoinfectiouschlorosisandchlorosisvirusesinfieldconditions AT komivisenyoakutse canfungalendophytessuppresstrialeurodesvaporariorumandthetransmissionoftomatoinfectiouschlorosisandchlorosisvirusesinfieldconditions AT komivisenyoakutse canfungalendophytessuppresstrialeurodesvaporariorumandthetransmissionoftomatoinfectiouschlorosisandchlorosisvirusesinfieldconditions |