Decreased Anti-Inflammatory Responses to Vitamin D in Neonatal Neutrophils
Neutrophil activity is prolonged in newborns, suggesting decreased exposure and/or responses to immunosuppressive modulators, such as 1,25-hydroxyvitamin D3 (1,25-vit D3). We hypothesized that 1,25-vit D3 suppresses neutrophil activation and that this response is impaired in newborns. Consistent wit...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2011/598345 |
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| author | Daniel Hirsch Faith E. Archer Meera Joshi-Kale Anna M. Vetrano Barry Weinberger |
| author_facet | Daniel Hirsch Faith E. Archer Meera Joshi-Kale Anna M. Vetrano Barry Weinberger |
| author_sort | Daniel Hirsch |
| collection | DOAJ |
| description | Neutrophil activity is prolonged in newborns, suggesting decreased exposure and/or responses to immunosuppressive modulators, such as 1,25-hydroxyvitamin D3 (1,25-vit D3). We hypothesized that 1,25-vit D3 suppresses neutrophil activation and that this response is impaired in newborns. Consistent with this, 1,25-vit D3 decreased LPS-induced expression of macrophage inflammatory protein-1β and VEGF in adult, but not neonatal, neutrophils. Expression of vitamin D receptor (VDR) and 25-hydroxyvitamin D3-1α-hydroxylase was reduced in neonatal, relative to adult neutrophils. Moreover, 1,25-vit D3 induced VDR gene expression in activated adult, but not neonatal, neutrophils. 1,25-vit D3 also suppressed expression of cyclooxygenase-2 and induced expression of 5-lipoxygenase in LPS-exposed adult neutrophils, while neonatal cells were not affected. 1,25-vit D3 had no effect on respiratory burst in either adult or neonatal cells. Anti-inflammatory activity of vitamin D is impaired in neonatal neutrophils, and this may be due to decreased expression of VDR and 1α-hydroxylase. Insensitivity to 1,25-vit D3 may contribute to chronic inflammation in neonates. |
| format | Article |
| id | doaj-art-673b51e6c3684a719f7fda535aeb2934 |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
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| series | Mediators of Inflammation |
| spelling | doaj-art-673b51e6c3684a719f7fda535aeb29342025-08-20T02:23:53ZengWileyMediators of Inflammation0962-93511466-18612011-01-01201110.1155/2011/598345598345Decreased Anti-Inflammatory Responses to Vitamin D in Neonatal NeutrophilsDaniel Hirsch0Faith E. Archer1Meera Joshi-Kale2Anna M. Vetrano3Barry Weinberger4Division of Neonatology, Department of Pediatrics, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, New Brunswick, NJ 08901, USADivision of Neonatology, Department of Pediatrics, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, New Brunswick, NJ 08901, USADivision of Neonatology, Department of Pediatrics, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, New Brunswick, NJ 08901, USADivision of Neonatology, Department of Pediatrics, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, New Brunswick, NJ 08901, USADivision of Neonatology, Department of Pediatrics, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, New Brunswick, NJ 08901, USANeutrophil activity is prolonged in newborns, suggesting decreased exposure and/or responses to immunosuppressive modulators, such as 1,25-hydroxyvitamin D3 (1,25-vit D3). We hypothesized that 1,25-vit D3 suppresses neutrophil activation and that this response is impaired in newborns. Consistent with this, 1,25-vit D3 decreased LPS-induced expression of macrophage inflammatory protein-1β and VEGF in adult, but not neonatal, neutrophils. Expression of vitamin D receptor (VDR) and 25-hydroxyvitamin D3-1α-hydroxylase was reduced in neonatal, relative to adult neutrophils. Moreover, 1,25-vit D3 induced VDR gene expression in activated adult, but not neonatal, neutrophils. 1,25-vit D3 also suppressed expression of cyclooxygenase-2 and induced expression of 5-lipoxygenase in LPS-exposed adult neutrophils, while neonatal cells were not affected. 1,25-vit D3 had no effect on respiratory burst in either adult or neonatal cells. Anti-inflammatory activity of vitamin D is impaired in neonatal neutrophils, and this may be due to decreased expression of VDR and 1α-hydroxylase. Insensitivity to 1,25-vit D3 may contribute to chronic inflammation in neonates.http://dx.doi.org/10.1155/2011/598345 |
| spellingShingle | Daniel Hirsch Faith E. Archer Meera Joshi-Kale Anna M. Vetrano Barry Weinberger Decreased Anti-Inflammatory Responses to Vitamin D in Neonatal Neutrophils Mediators of Inflammation |
| title | Decreased Anti-Inflammatory Responses to Vitamin D in Neonatal Neutrophils |
| title_full | Decreased Anti-Inflammatory Responses to Vitamin D in Neonatal Neutrophils |
| title_fullStr | Decreased Anti-Inflammatory Responses to Vitamin D in Neonatal Neutrophils |
| title_full_unstemmed | Decreased Anti-Inflammatory Responses to Vitamin D in Neonatal Neutrophils |
| title_short | Decreased Anti-Inflammatory Responses to Vitamin D in Neonatal Neutrophils |
| title_sort | decreased anti inflammatory responses to vitamin d in neonatal neutrophils |
| url | http://dx.doi.org/10.1155/2011/598345 |
| work_keys_str_mv | AT danielhirsch decreasedantiinflammatoryresponsestovitamindinneonatalneutrophils AT faithearcher decreasedantiinflammatoryresponsestovitamindinneonatalneutrophils AT meerajoshikale decreasedantiinflammatoryresponsestovitamindinneonatalneutrophils AT annamvetrano decreasedantiinflammatoryresponsestovitamindinneonatalneutrophils AT barryweinberger decreasedantiinflammatoryresponsestovitamindinneonatalneutrophils |